Binding of the human cytomegalovirus (HCMV) tegument protein UL69 to UAP56/URH49 is not required for efficient replication of HCMV

Journal of Virology

Volumn 84, Issue 18, 2010, Pages 9649-9654

  Kronemann, Daniel ^a   Hagemeier, Stacy R ^a,b   Cygnar, Dan ^a   Phillips, Stacia ^a   Bresnahan, Wade A ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; PROTEIN UAP56; PROTEIN UL69; PROTEIN URH49; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA; DDX39A PROTEIN, HUMAN; DDX39B PROTEIN, HUMAN; DEAD BOX PROTEIN; MUTANT PROTEIN; PROTEIN BINDING; PUL69 PROTEIN, HUMAN HERPESVIRUS 5; TRANSACTIVATOR PROTEIN;

ARTICLE; BINDING COMPETITION; CELL NUCLEUS; CONTROLLED STUDY; DELETION MUTANT; HUMAN CYTOMEGALOVIRUS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; VIROGENESIS; VIRUS MUTANT; VIRUS REPLICATION; ANIMAL; CELL LINE; CYTOMEGALOVIRUS; GENE DELETION; GENETICS; HOST PATHOGEN INTERACTION; HUMAN; METABOLISM; MOUSE; PHYSIOLOGY;

ANIMALS; CELL LINE; CYTOMEGALOVIRUS; DEAD-BOX RNA HELICASES; GENE DELETION; HOST-PATHOGEN INTERACTIONS; HUMANS; MICE; MUTANT PROTEINS; PROTEIN BINDING; TRANS-ACTIVATORS; VIRUS REPLICATION;

EID: 77956020947     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.00669-10     Document Type: Article

References (22)

1. Aoyagi, M., M. Gaspar, and T. E. Shenk. 2010. Human cytomegalovirus UL69 protein facilitates translation by associating with the mRNA cap-binding complex and excluding 4EBP1. Proc. Natl. Acad. Sci. U. S. A. 107:2640-2645.
2. Bortvin, A., and F. Winston. 1996. Evidence that Spt6p controls chromatin structure by a direct interaction with histones. Science 272:1473-1476. (Pubitemid 26199996)
3. Cantrell, S. R., and W. A. Bresnahan. 2006. Human cytomegalovirus (HCMV) UL82 gene product (pp71) relieves hDaxx-mediated repression of HCMV replication. J. Virol. 80:6188-6191.
4. Endoh, M., W. Zhu, J. Hasegawa, H. Watanabe, D. K. Kim, M. Aida, N. Inukai, T. Narita, T. Yamada, A. Furuya, H. Sato, Y. Yamaguchi, S. S. Mandal, D. Reinberg, T. Wada, and H. Handa. 2004. Human Spt6 stimulates transcription elongation by RNA polymerase II in vitro. Mol. Cell. Biol. 24:3324-3336.
5. Hayashi, M. L., C. Blankenship, and T. Shenk. 2000. Human cytomegalovirus UL69 protein is required for efficient accumulation of infected cells in the G1 phase of the cell cycle. Proc. Natl. Acad. Sci. U. S. A. 97:2692-2696.
6. Huh, Y. H., Y. E. Kim, E. T. Kim, J. J. Park, M. J. Song, H. Zhu, G. S. Hayward, and J. H. Ahn. 2008. Binding STAT2 by the acidic domain of human cytomegalovirus IE1 promotes viral growth and is negatively regulated by SUMO. J. Virol. 82:10444-10454.
7. Johnson, L. A., and R. M. Sandri-Goldin. 2009. Efficient nuclear export of herpes simplex virus 1 transcripts requires both RNA binding by ICP27 and ICP27 interaction with TAP/NXF1. J. Virol. 83:1184-1192.
8. Kalejta, R. F. 2008. Functions of human cytomegalovirus tegument proteins prior to immediate early gene expression. Curr. Top. Microbiol. Immunol. 325:101-115.
9. Kalejta, R. F. 2008. Tegument proteins of human cytomegalovirus. Microbiol. Mol. Biol. Rev. 72:249-265.
10. Lischka, P., O. Rosorius, E. Trommer, and T. Stamminger. 2001. A novel transferable nuclear export signal mediates CRM1-independent nucleocytoplasmic shuttling of the human cytomegalovirus transactivator protein pUL69. EMBO J. 20:7271-7283.
11. Lischka, P., M. Thomas, Z. Toth, R. Mueller, and T. Stamminger. 2007. Multimerization of human cytomegalovirus regulatory protein UL69 via a domain that is conserved within its herpesvirus homologues. J. Gen. Virol. 88:405-410.
12. Lischka, P., Z. Toth, M. Thomas, R. Mueller, and T. Stamminger. 2006. The UL69 transactivator protein of human cytomegalovirus interacts with DEXD/H-Box RNA helicase UAP56 to promote cytoplasmic accumulation of unspliced RNA. Mol. Cell. Biol. 26:1631-1643.
13. Lu, M., and T. Shenk. 1999. Human cytomegalovirus UL69 protein induces cells to accumulate in G1 phase of the cell cycle. J. Virol. 73:676-683.
14. Luo, M. L., Z. Zhou, K. Magni, C. Christoforides, J. Rappsilber, M. Mann, and R. Reed. 2001. Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly. Nature 413:644-647.
15. Pryor, A., L. Tung, Z. Yang, F. Kapadia, T. H. Chang, and L. F. Johnson. 2004. Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExH/D box protein that is highly related to the mRNA export protein UAP56. Nucleic Acids Res. 32:1857-1865.
16. Rechter, S., G. M. Scott, J. Eickhoff, K. Zielke, S. Auerochs, R. Muller, T. Stamminger, W. D. Rawlinson, and M. Marschall. 2009. Cyclin-dependent kinases phosphorylate the cytomegalovirus RNA export protein pUL69 and modulate its nuclear localization and activity. J. Biol. Chem. 284:8605-8613.
17. Smith, G. A., and L. W. Enquist. 1999. Construction and transposon mutagenesis in Escherichia coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus. J. Virol. 73:6405-6414.
18. Thomas, M., S. Rechter, J. Milbradt, S. Auerochs, R. Muller, T. Stamminger, and M. Marschall. 2009. Cytomegaloviral protein kinase pUL97 interacts with the nuclear mRNA export factor pUL69 to modulate its intranuclear localization and activity. J. Gen. Virol. 90:567-578.
19. Toth, Z., P. Lischka, and T. Stamminger. 2006. RNA-binding of the human cytomegalovirus transactivator protein UL69, mediated by arginine-rich motifs, is not required for nuclear export of unspliced RNA. Nucleic Acids Res. 34:1237-1249.
20. Toth, Z., and T. Stamminger. 2008. The human cytomegalovirus regulatory protein UL69 and its effect on mRNA export. Front. Biosci. 13:2939-2949.
21. Winkler, M., T. aus Dem Siepen, and T. Stamminger. 2000. Functional interaction between pleiotropic transactivator pUL69 of human cytomegalovirus and the human homolog of yeast chromatin regulatory protein SPT6. J. Virol. 74:8053-8064.
22. Yu, D., G. A. Smith, L. W. Enquist, and T. Shenk. 2002. Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL/IRL13 gene. J. Virol. 76:2316-2328.