On-chip photoactivation of heterologously expressed rhodopsin allows kinetic analysis of G-protein signaling by surface plasmon resonance spectroscopy

Analytical and Bioanalytical Chemistry

Volumn 397, Issue 7, 2010, Pages 2967-2976

  Komolov, Konstantin E ^a   Aguilà, Mònica ^b   Toledo, Darwin ^b   Manyosa, Joan ^c   Garriga, Pere ^b   Koch, Karl Wilhelm ^a  

^a UNIVERSITY OF OLDENBURG   (Germany)

^b UNIVERSITAT POLITÈCNICA DE CATALUNYA   (Spain)

^c UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

G protein coupled receptor; Rhodopsin; Signal transduction; Surface plasmon resonance

Indexed keywords

ASSOCIATION AND DISSOCIATION KINETICS; EXPERIMENTAL CONDITIONS; EXPERIMENTAL PARAMETERS; FUNCTIONAL CONFORMATIONS; FUNCTIONAL OPERATION; FUNCTIONAL TEST; G PROTEIN; G-PROTEIN COUPLED RECEPTORS; KINETIC ANALYSIS; LOW LEVEL; NATIVE CONFORMATION; ON CHIPS; PHOTOACTIVATION; PROTEIN INTERACTION; RHODOPSIN; SENSOR CHIPS; SURFACE PLASMON RESONANCE SPECTROSCOPY; SURFACE PLASMONS; TRANSMEMBRANES;

BACTERIOPHAGES; BIOSENSORS; DETERGENTS; MASS SPECTROMETRY; PARTICLE DETECTORS; PLASMONS; PROTEINS; RAMAN SPECTROSCOPY; SELF ASSEMBLY; SURFACE PLASMON RESONANCE;

SIGNAL TRANSDUCTION;

GUANINE NUCLEOTIDE BINDING PROTEIN; RHODOPSIN; PROTEIN BINDING;

ANIMAL; CATTLE; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; CONFERENCE PAPER; EVALUATION; GENE EXPRESSION; GENETIC PROCEDURES; GENETICS; KINETICS; METABOLISM; METHODOLOGY; PHOTOCHEMISTRY; PROTEIN BINDING; RADIATION EXPOSURE; SIGNAL TRANSDUCTION; SURFACE PLASMON RESONANCE; BOVINAE; CHLOROCEBUS AETHIOPS; COS 1 CELL LINE; EVALUATION STUDY; PROCEDURES; RADIATION RESPONSE;

ANIMALS; BIOSENSING TECHNIQUES; CATTLE; CERCOPITHECUS AETHIOPS; COS CELLS; GENE EXPRESSION; GTP-BINDING PROTEINS; KINETICS; PHOTOCHEMICAL PROCESSES; PROTEIN BINDING; RHODOPSIN; SIGNAL TRANSDUCTION; SURFACE PLASMON RESONANCE;

EID: 77955985486     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-010-3876-4     Document Type: Conference Paper

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