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Volumn 398, Issue 1, 2010, Pages 547-554

A simple, sensitive and selective quantum-dot-based western blot method for the simultaneous detection of multiple targets from cell lysates

Author keywords

Multiplexing; Protein A G; Quantum dot; Western blot

Indexed keywords

BIOCONJUGATES; BIOLOGICAL APPLICATIONS; CELL LYSATES; CELLULAR PROTEINS; DETECTION AGENTS; EMISSION BANDS; ENHANCED CHEMILUMINESCENCES; EXPERIMENTAL DESIGN; FLUORESCENT DYES; FLUORESCENT NANOPARTICLES; MULTIPLE TARGETS; NON-SPECIFIC BINDING; PHOTO-STABILITY; QUANTUM DOT; QUANTUM DOTS; SECONDARY ANTIBODIES; SIMULTANEOUS DETECTION; STOKES SHIFT; WESTERN BLOT METHOD; WESTERN BLOTS; WESTERN BLOTTING;

EID: 77955980439     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-010-3908-0     Document Type: Article
Times cited : (15)

References (22)
  • 1
    • 71049147493 scopus 로고    scopus 로고
    • Review: Biofunctionalized quantum dots in biology and medicine
    • 10.1155/2009/815734
    • S Mazumder R Dey MK Mitra S Mukherjee GC Das 2009 Review: biofunctionalized quantum dots in biology and medicine J Nanomater 2009 1 17 10.1155/2009/815734
    • (2009) J Nanomater , vol.2009 , pp. 1-17
    • Mazumder, S.1    Dey, R.2    Mitra, M.K.3    Mukherjee, S.4    Das, G.C.5
  • 2
    • 49049088505 scopus 로고    scopus 로고
    • Semiconductor quantum dots and metal nanoparticles: Syntheses, optical properties, and biological applications
    • 10.1007/s00216-008-2185-7 1:CAS:528:DC%2BD1cXptFSqtrc%3D
    • V Biju T Itoh A Anas A Sujith M Ishikawa 2008 Semiconductor quantum dots and metal nanoparticles: syntheses, optical properties, and biological applications Anal Bioanal Chem 391 2469 2495 10.1007/s00216-008-2185-7 1:CAS:528:DC%2BD1cXptFSqtrc%3D
    • (2008) Anal Bioanal Chem , vol.391 , pp. 2469-2495
    • Biju, V.1    Itoh, T.2    Anas, A.3    Sujith, A.4    Ishikawa, M.5
  • 3
    • 67651250058 scopus 로고    scopus 로고
    • QDs versus Alexa: Reality of promising tools for immunocytochemistry
    • 10.1186/1477-3155-7-4
    • H Monton C Nogues E Rossinyol O Castell M Roldan 2009 QDs versus Alexa: reality of promising tools for immunocytochemistry J Nanobiotechnol 7 4 10.1186/1477-3155-7-4
    • (2009) J Nanobiotechnol , vol.7 , pp. 4
    • Monton, H.1    Nogues, C.2    Rossinyol, E.3    Castell, O.4    Roldan, M.5
  • 4
    • 54749138351 scopus 로고    scopus 로고
    • Semiconductor quantum dots for bioanalysis
    • 10.1002/anie.200800169 1:CAS:528:DC%2BD1cXht1GltLzK
    • R Gill M Zayats I Willner 2008 Semiconductor quantum dots for bioanalysis Angew Chem Int Edit 47 7602 7625 10.1002/anie.200800169 1:CAS:528: DC%2BD1cXht1GltLzK
    • (2008) Angew Chem Int Edit , vol.47 , pp. 7602-7625
    • Gill, R.1    Zayats, M.2    Willner, I.3
  • 5
    • 0032566763 scopus 로고    scopus 로고
    • Quantum dot bioconjugates for ultrasensitive nonisotopic detection
    • DOI 10.1126/science.281.5385.2016
    • WCW Chan SM Nie 1998 Quantum dot bioconjugates for ultrasensitive nonisotopic detection Science 281 2016 2018 10.1126/science.281.5385.2016 1:CAS:528:DyaK1cXmtlKnsrk%3D (Pubitemid 28475486)
    • (1998) Science , vol.281 , Issue.5385 , pp. 2016-2018
    • Chan, W.C.W.1    Nie, S.2
  • 6
    • 32044461510 scopus 로고    scopus 로고
    • Fluorescent labels for proteomics and genomics
    • DOI 10.1016/j.cbpa.2006.01.005, PII S1367593106000056, Proteins and Genomics
    • A Waggoner 2006 Fluorescent labels for proteomics and genomics Curr Opin Chem Biol 10 62 66 10.1016/j.cbpa.2006.01.005 1:CAS:528:DC%2BD28XhtlGntLo%3D (Pubitemid 43202289)
    • (2006) Current Opinion in Chemical Biology , vol.10 , Issue.1 , pp. 62-66
    • Waggoner, A.1
  • 7
    • 69649109363 scopus 로고    scopus 로고
    • Potential clinical applications of quantum dots
    • 1:CAS:528:DC%2BD1cXps1Wjt7o%3D
    • IL Medintz H Mattoussi AR Clapp 2008 Potential clinical applications of quantum dots Int J Nanomed 3 151 167 1:CAS:528:DC%2BD1cXps1Wjt7o%3D
    • (2008) Int J Nanomed , vol.3 , pp. 151-167
    • Medintz, I.L.1    Mattoussi, H.2    Clapp, A.R.3
  • 8
    • 20144379798 scopus 로고    scopus 로고
    • Quantum dot bioconjugates for imaging, labelling and sensing
    • DOI 10.1038/nmat1390
    • IL Medintz HT Uyeda ER Goldman H Mattoussi 2005 Quantum dot bioconjugates for imaging, labelling and sensing Nat Mater 4 435 446 10.1038/nmat1390 1:CAS:528:DC%2BD2MXks1Cit7k%3D (Pubitemid 40774047)
    • (2005) Nature Materials , vol.4 , Issue.6 , pp. 435-446
    • Medintz, I.L.1    Uyeda, H.T.2    Goldman, E.R.3    Mattoussi, H.4
  • 11
    • 67650232948 scopus 로고    scopus 로고
    • Simultaneous and sensitive determination of multiplex chemical residues based on multicolour quantum dot probes
    • 10.1016/j.bios.2009.05.031 1:CAS:528:DC%2BD1MXoslahsbw%3D
    • C Peng Z Li Y Zhu W Chen Y Yuan L Liu Q Li D Xu R Qiao L Wang S Zhu S Jin C Xu 2009 Simultaneous and sensitive determination of multiplex chemical residues based on multicolour quantum dot probes Biosens Bioelectron 24 3657 3662 10.1016/j.bios.2009.05.031 1:CAS:528:DC%2BD1MXoslahsbw%3D
    • (2009) Biosens Bioelectron , vol.24 , pp. 3657-3662
    • Peng, C.1    Li, Z.2    Zhu, Y.3    Chen, W.4    Yuan, Y.5    Liu, L.6    Li, Q.7    Xu, D.8    Qiao, R.9    Wang, L.10    Zhu, S.11    Jin, S.12    Xu, C.13
  • 12
    • 34547579309 scopus 로고    scopus 로고
    • Characterization of the functional binding properties of antibody conjugated quantum dots
    • DOI 10.1021/nl062706i
    • S Pathak MC Davidson GA Silva 2007 Characterization of the functional binding properties of antibody conjugated quantum dots Nano Lett 7 1839 1845 10.1021/nl062706i 1:CAS:528:DC%2BD2sXlvVymsbc%3D (Pubitemid 47197555)
    • (2007) Nano Letters , vol.7 , Issue.7 , pp. 1839-1845
    • Pathak, S.1    Davidson, M.C.2    Silva, G.A.3
  • 13
    • 46549089870 scopus 로고    scopus 로고
    • Western blot analysis using metal-nitrilotriacetate conjugated CdSe/ZnS quantum dots
    • 10.1016/j.ab.2008.04.032 1:CAS:528:DC%2BD1cXnt1amtrc%3D
    • MJ Kim HY Park J Kim J Ryu S Hong SJ Han R Song 2008 Western blot analysis using metal-nitrilotriacetate conjugated CdSe/ZnS quantum dots Anal Biochem 379 124 126 10.1016/j.ab.2008.04.032 1:CAS:528:DC%2BD1cXnt1amtrc%3D
    • (2008) Anal Biochem , vol.379 , pp. 124-126
    • Kim, M.J.1    Park, H.Y.2    Kim, J.3    Ryu, J.4    Hong, S.5    Han, S.J.6    Song, R.7
  • 14
    • 67651230765 scopus 로고    scopus 로고
    • Single particle quantum dot imaging achieves ultrasensitive detection capabilities for western immunoblot analysis
    • 10.1021/nn9000353 1:CAS:528:DC%2BD1MXmtVGltL8%3D
    • B Scholl HY Liu BR Long OJT McCarty T O'Hare BJ Druker TQ Vu 2009 Single particle quantum dot imaging achieves ultrasensitive detection capabilities for western immunoblot analysis ACS Nano 3 1318 1328 10.1021/nn9000353 1:CAS:528:DC%2BD1MXmtVGltL8%3D
    • (2009) ACS Nano , vol.3 , pp. 1318-1328
    • Scholl, B.1    Liu, H.Y.2    Long, B.R.3    McCarty, O.J.T.4    O'Hare, T.5    Druker, B.J.6    Vu, T.Q.7
  • 15
    • 32944473614 scopus 로고    scopus 로고
    • Bioconjugation of quantum dot luminescent probes for Western blot analysis
    • 10.2144/000112004 1:CAS:528:DC%2BD2MXhtFWgs7fM
    • SC Makrides C Gasbarro JM Bello 2005 Bioconjugation of quantum dot luminescent probes for Western blot analysis Biotechniques 39 501 506 10.2144/000112004 1:CAS:528:DC%2BD2MXhtFWgs7fM
    • (2005) Biotechniques , vol.39 , pp. 501-506
    • Makrides, S.C.1    Gasbarro, C.2    Bello, J.M.3
  • 16
    • 0037083698 scopus 로고    scopus 로고
    • Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays
    • DOI 10.1021/ac010662m
    • ER Goldman GP Anderson PT Tran H Mattoussi PT Charles JM Mauro 2002 Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays Anal Chem 74 841 847 10.1021/ac010662m 1:CAS:528:DC%2BD38XlslSn (Pubitemid 34160551)
    • (2002) Analytical Chemistry , vol.74 , Issue.4 , pp. 841-847
    • Goldman, E.R.1    Anderson, G.P.2    Tran, P.T.3    Mattoussi, H.4    Charles, P.T.5    Mauro, J.M.6
  • 17
    • 57749186224 scopus 로고    scopus 로고
    • Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system
    • 10.1016/j.biomaterials.2008.11.007 1:CAS:528:DC%2BD1cXhsFCisrbL
    • YT Lim MY Cho JM Lee SJ Chung BH Chung 2009 Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system Biomaterials 30 1197 1204 10.1016/j.biomaterials.2008.11.007 1:CAS:528:DC%2BD1cXhsFCisrbL
    • (2009) Biomaterials , vol.30 , pp. 1197-1204
    • Lim, Y.T.1    Cho, M.Y.2    Lee, J.M.3    Chung, S.J.4    Chung, B.H.5
  • 18
    • 33847115247 scopus 로고    scopus 로고
    • Quantum dot-based immunosensor for the detection of prostate-specific antigen using fluorescence microscopy
    • DOI 10.1016/j.talanta.2006.07.027, PII S0039914006005194
    • K Kerman T Endo M Tsukamoto M Chikae Y Takamura E Tamiya 2007 Quantum dot-based immunosensor for the detection of prostate-specific antigen using fluorescence microscopy Talanta 71 1494 1499 10.1016/j.talanta.2006.07.027 1:CAS:528:DC%2BD2sXit1yltrk%3D (Pubitemid 46282821)
    • (2007) Talanta , vol.71 , Issue.4 , pp. 1494-1499
    • Kerman, K.1    Endo, T.2    Tsukamoto, M.3    Chikae, M.4    Takamura, Y.5    Tamiya, E.6
  • 19
    • 70349260136 scopus 로고    scopus 로고
    • Enhancement of sensitivity and specificity of the fluoroimmunoassay of Hepatitis B virus surface antigen through "flexible" coupling between quantum dots and antibody
    • 10.1016/j.talanta.2009.06.061 1:CAS:528:DC%2BD1MXhtF2hsbrF
    • Q Zeng Y Zhang K Song X Kong MCG Aalders H Zhang 2009 Enhancement of sensitivity and specificity of the fluoroimmunoassay of Hepatitis B virus surface antigen through "flexible" coupling between quantum dots and antibody Talanta 80 307 312 10.1016/j.talanta.2009.06.061 1:CAS:528: DC%2BD1MXhtF2hsbrF
    • (2009) Talanta , vol.80 , pp. 307-312
    • Zeng, Q.1    Zhang, Y.2    Song, K.3    Kong, X.4    Aalders, M.C.G.5    Zhang, H.6
  • 20
    • 0023914449 scopus 로고
    • Chimeric IgG-binding receptors engineered from staphylococcal protein-A and staphylococcal protein-G
    • 1:CAS:528:DyaL1cXhs1Grtrw%3D
    • M Eliasson A Olsson E Palmcrantz K Wiberg M Inganas B Guss M Lindberg M Uhlen 1988 Chimeric IgG-binding receptors engineered from staphylococcal protein-A and staphylococcal protein-G J Biol Chem 263 4323 4327 1:CAS:528:DyaL1cXhs1Grtrw%3D
    • (1988) J Biol Chem , vol.263 , pp. 4323-4327
    • Eliasson, M.1    Olsson, A.2    Palmcrantz, E.3    Wiberg, K.4    Inganas, M.5    Guss, B.6    Lindberg, M.7    Uhlen, M.8
  • 21
    • 43549120013 scopus 로고    scopus 로고
    • Quantum dot-based protein micro- and nanoarrays for detection of prostate cancer biomarkers
    • DOI 10.1002/pmic.200701072
    • A Gokarna LH Jin JS Hwang YH Cho YT Lim BH Chung SH Youn DS Choi JH Lim 2008 Quantum dot-based protein micro- and nanoarrays for detection of prostate cancer biomarkers Proteomics 8 1809 1818 10.1002/pmic.200701072 1:CAS:528:DC%2BD1cXlvFeltLY%3D (Pubitemid 351678281)
    • (2008) Proteomics , vol.8 , Issue.9 , pp. 1809-1818
    • Gokarna, A.1    Jin, L.-H.2    Jun, S.H.3    Cho, Y.-H.4    Yong, T.L.5    Bong, H.C.6    Seong, H.Y.7    Dong, S.C.8    Jung, H.L.9


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