Gene expression changes in primary human nasal epithelial cells exposed to formaldehyde in vitro

Toxicology Letters

Volumn 198, Issue 2, 2010, Pages 289-295

  Neuss, Simone ^a   Holzmann, Karlheinz ^b   Speit, Günter ^a  

^a UNIVERSITY OF ULM   (Germany)

^b UNIVERSITY HOSPITAL ULM   (Germany)

Author keywords

Comet assay; DNA repair; DNA protein crosslinks; Microarray; RNA levels

Indexed keywords

DNA; FORMALDEHYDE;

ARTICLE; CELL MIGRATION; CELL VIABILITY; COMET ASSAY; CONTROLLED STUDY; CROSS LINKING; DETOXIFICATION; EPITHELIUM CELL; GAMMA RADIATION; GENE EXPRESSION; GENETIC ANALYSIS; GENOTOXICITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; MICROARRAY ANALYSIS; PRIORITY JOURNAL; PROTEIN INDUCTION; TIME;

CELL LINE; COMET ASSAY; DNA DAMAGE; DNA REPAIR; DNA-BINDING PROTEINS; DOSE-RESPONSE RELATIONSHIP, DRUG; EPITHELIAL CELLS; FORMALDEHYDE; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; MUTAGENS; NASAL MUCOSA; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS;

EID: 77955918349     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxlet.2010.07.010     Document Type: Article

References (31)

1. Andersen M.E., Clewell H.J., Bermudez E., Willson G.A., Thomas R.S. Genomic signatures and dose dependent transitions in nasal epithelial responses to inhaled formaldehyde in the rat. Toxicol. Sci. 2008, 105:368-383.
2. Barker S., Weinfeld M., Murray D. DNA-protein crosslinks: their induction, repair, and biological consequences. Mutat. Res. 2005, 589:111-135.
3. Casanova M., Morgan K.T., Gross E.A., Moss O.R., Heck H.A. DNA-protein cross-links and cell replication at specific sites in the nose of F344 rats exposed subchronically to formaldehyde. Fundam. Appl. Toxicol. 1994, 23:525-536.
4. Conolly R.B., Kimbell J.S., Janszen D., Schlosser P.M., Kalisak D., Preston J., Miller F.J. Biologically motivated computational modeling of formaldehyde carcinogenicity in the F344 rat. Toxicol. Sci. 2003, 75:432-447.
5. Ellinger-Ziegelbauer H., Aubrecht J., Kleinjans J.C., Ahr H.J. Application of toxicogenomics to study mechanisms of genotoxicity and carcinogenicity. Toxicol. Lett. 2009, 186:36-44.
6. Ellinger-Ziegelbauer H., Fostel J.M., Aruga C., Bauer D., Boitier E., Deng S., Dickinson D., Le Fevre A.C., Fornace A.J., Grenet O., Gu Y., Hoflack J.C., Shiiyama M., Smith R., Snyder R.D., Spire C., Tanaka G., Aubrecht J. Characterization and interlaboratory comparison of a gene expression signature for differentiating genotoxic mechanisms. Toxicol. Sci. 2009, 110:341-352.
7. Hester S.D., Barry W.T., Zou F., Wolf D.C. Transcriptomic analysis of F344 rat nasal epithelium suggests that the lack of carcinogenic response to glutaraldehyde is due to its greater toxicity compared to formaldehyde. Toxicol. Pathol. 2005, 33:415-424.
8. Hester S.D., Benavides G.B., Yoon L., Morgan K.T., Zou F., Barry W., Wolf D.C. Formaldehyde-induced gene expression in F344 rat nasal respiratory epithelium. Toxicology 2003, 187:13-24.
9. IARC, 2006. Formaldehyde, 2-butoxyethanol and 1-tert-butoxypropan-2-ol, pp. 1-478.
10. Irizarry R.A., Hobbs B., Collin F., Beazer-Barclay Y.D., Antonellis K.J., Scherf U., Speed T.P. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 2003, 4:249-264.
11. Kerns W.D., Pavkov K.L., Donofrio D.J., Gralla E.J., Swenberg J.A. Carcinogenicity of formaldehyde in rats and mice after long-term inhalation exposure. Cancer Res. 1983, 43:4382-4392.
12. Li G.Y., Lee H.Y., Shin H.S., Kim H.Y., Lim C.H., Lee B.H. Identification of gene markers for formaldehyde exposure in humans. Environ. Health Perspect. 2007, 115:1460-1466.
13. Merk O., Speit G. Significance of formaldehyde-induced DNA-protein crosslinks for mutagenesis. Environ. Mol. Mutagen. 1998, 32:260-268.
14. Monticello T.M., Miller F.J., Morgan K.T. Regional increases in rat nasal epithelial cell proliferation following acute and subchronic inhalation of formaldehyde. Toxicol. Appl. Pharmacol. 1991, 111:409-421.
15. Monticello T.M., Morgan K.T., Everitt J.I., Popp J.A. Effects of formaldehyde gas on the respiratory tract of rhesus monkeys. Pathology and cell proliferation. Am. J. Pathol. 1989, 134:515-527.
16. Monticello T.M., Swenberg J.A., Gross E.A., Leininger J.R., Kimbell J.S., Seilkop S., Starr T.B., Gibson J.E., Morgan K.T. Correlation of regional and nonlinear formaldehyde-induced nasal cancer with proliferating populations of cells. Cancer Res. 1996, 56:1012-1022.
17. Neuss S., Speit G. Further characterization of the genotoxicity of formaldehyde in vitro by the sister chromatid exchange test and co-cultivation experiments. Mutagenesis 2008, 23:355-357.
18. Neuss S., Zeller J., Ma-Hock L., Speit G. Inhalation of formaldehyde does not induce genotoxic effects in broncho-alveolar lavage (BAL) cells of rats. Mutat. Res. 2009, 695:61-68.
19. Neuss S., Moepps B., Speit G. Exposure of human nasal epithelial cells to formaldehyde does not lead to DNA damage in lymphocytes after co-cultivation. Mutagenesis 2010, (Epub ahead of print).
20. Nuwaysir E.F., Bittner M., Trent J., Barrett J.C., Afshari C.A. Microarrays and toxicology: the advent of toxicogenomics. Mol. Carcinog. 1999, 24:153-159.
21. Pfuhler S., Wolf H.U. Detection of DNA-crosslinking agents with the alkaline comet assay. Environ. Mol. Mutagen. 1996, 27:196-201.
22. Quievryn G., Zhitkovich A. Loss of DNA-protein crosslinks from formaldehyde-exposed cells occurs through spontaneous hydrolysis and an active repair process linked to proteosome function. Carcinogenesis 2000, 21:1573-1580.
23. Ridpath J.R., Nakamura A., Tano K., Luke A.M., Sonoda E., Arakawa H., Buerstedde J.M., Gillespie D.A., Sale J.E., Yamazoe M., Bishop D.K., Takata M., Takeda S., Watanabe M., Swenberg J.A., Nakamura J. Cells deficient in the FANC/BRCA pathway are hypersensitive to plasma levels of formaldehyde. Cancer Res. 2007, 67:11117-11122.
24. Schmid O., Speit G. Genotoxic effects induced by formaldehyde in human blood and implications for the interpretation of biomonitoring studies. Mutagenesis 2007, 22:69-74.
25. Speit G., Hartmann A. The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair. Methods Mol. Biol. 2006, 314:275-286.
26. Speit G., Neuss S., Schmid O. The human lung cell line A549 does not develop adaptive protection against the DNA-damaging action of formaldehyde. Environ. Mol. Mutagen. 2009, 51:130-137.
27. Speit G., Schmid O., Neuss S., Schutz P. Genotoxic effects of formaldehyde in the human lung cell line A549 and in primary human nasal epithelial cells. Environ. Mol. Mutagen. 2008, 49:300-307.
28. Speit G., Schutz P., Hogel J., Schmid O. Characterization of the genotoxic potential of formaldehyde in V79 cells. Mutagenesis 2007, 22:387-394.
29. Speit G., Zeller J., Schmid O., Elhajouji A., Ma-Hock L., Neuss S. Inhalation of formaldehyde does not induce systemic genotoxic effects in rats. Mutat. Res. 2009, 677:76-85.
30. Wang A., Robertson J.L., Holladay S.D., Tennant A.H., Lengi A.J., Ahmed S.A., Huckle W.R., Kligerman A.D. Measurement of DNA damage in rat urinary bladder transitional cells: improved selective harvest of transitional cells and detailed Comet assay protocols. Mutat. Res. 2007, 634:51-59.
31. Zeeberg B.R., Feng W., Wang G., Wang M.D., Fojo A.T., Sunshine M., Narasimhan S., Kane D.W., Reinhold W.C., Lababidi S., Bussey K.J., Riss J., Barrett J.C., Weinstein J.N. GoMiner: a resource for biological interpretation of genomic and proteomic data. Genome Biol. 2003, 4:R28.