Assessment of genetic damage in inflammatory, precancerous, and cancerous pathologies of the esophagus using the comet assay

Genetic Testing and Molecular Biomarkers

Volumn 14, Issue 4, 2010, Pages 477-482

  Vasavi, Mohan ^a   Vedicherala, Bhavani ^b   Vattam, Kiran K ^a   Ahuja, Yog R ^c   Hasan, Qurratulain ^a,b  

^a KAMINENI HOSPITALS   (India)

^b Bhagwan Mahavir Medical Research Centre   (India)

^c Vasavi Medical and Research Centre ^*  (India)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL CONSUMPTION; ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; ESOPHAGUS CANCER; GASTROINTESTINAL DISEASE; HUMAN; HUMAN TISSUE; INDIA; MAJOR CLINICAL STUDY; PATHOLOGY; PRECANCER; RISK FACTOR; SMOKING; UPPER GASTROINTESTINAL TRACT;

BARRETT ESOPHAGUS; CARCINOMA, SQUAMOUS CELL; CASE-CONTROL STUDIES; COMET ASSAY; DNA DAMAGE; ESOPHAGEAL NEOPLASMS; ESOPHAGITIS; ESOPHAGUS; HUMANS; INFLAMMATION; PRECANCEROUS CONDITIONS; SMOKING;

NICOTIANA TABACUM;

EID: 77955899556     PISSN: 19450265     EISSN: 19450257     Source Type: Journal    
DOI: 10.1089/gtmb.2010.0006     Document Type: Article

References (37)

1. Ahuja YR, Saran R (1999) Alkaline single cell gel electrophoresis assay. I Protocol J Cytol Genet 34:57-62.
2. Ahuja YR, Saran R (2001) Potential of single cell gel electrophoresis assay (comet assay) in heavy ion radiation biology. Int J Hum Genet 2:151-156.
3. Ataian Y, Krebs JE (2006) Five repair pathways in one context: chromatin modification during DNA repair. Biochem Cell Biol 84:490-504.
4. Fracassoa ME, Doriaa D, Carrierib M, et al. (2009) DNA singleand double-strand breaks by alkaline- and immuno-comet assay in lymphocytes of workers exposed to styrene. Toxicol Lett 185:9-15.
5. Goud KI, Shankarappa K, Vijayshree B, et al. (2001) DNA damage and repair studies in individuals working with photocopying machines. Int J Hum Genet 2:139-143.
6. Hoffmann H, Hogel J, Speit G (2005) The effect of smoking on DNA effects in the comet assay: a meta-analysis. Mutagenesis 20:455-466. (Pubitemid 41816533)
7. Hopkins JM, Evans HJ (1980) Cigarette smoke-induced DNA damage and lung cancer risks. Nature 283:388-390.
8. Jaiswal M, Anuradha G, Rajeswari N, et al. (1995) Single cell gel electrophoresis assay (comet assay) its importance in human biology. Int J Anthropol 10:177-181.
9. Jolly AJ, Wild CP, Hardie LJ (2004) Acid and bile salts induce DNA damage in human oesophageal cell lines. Mutagenesis 19:319-324.
10. Kram JJD, Albertini RJ, Branda RF, et al. (1993) Measurement of chromosomal aberrations, sister chromatid exchange, hprt mutations and DNA adducts in peripheral lymphocytes of human populations at increased risk for cancer. Env Health Perspect 101:121-125.
11. Lamarche F, Gonthier B, Signorini N, et al. (2004) Impact of ethanol and acetaldehyde on DNA and cell viability of cultured neurones. Cell Biol Toxicol 20:361-374.
12. Lemiere S, Cossu-Leguille C, Bispo A, et al. (2005). DNA damage measured by the single-cell gel electrophoresis (Comet) assay in mammals fed with mussels contaminated by the ''Erika'' oilspill. Mutat Res 581:11-21.
13. López-Novoa JM, Nieto MA (2009) Inflammation and EMT: an alliance toward organ fibrosis and cancer progression EMBO Mol Med 1:303-314.
14. Malini J, Anuradha G, Rajeswari N, et al. (1994) Comet assay on cervical epithelial cells and leucocytes of patients with precancerous and cancerous lesions of the cervix. Med Sci Res 22:879-881.
15. Maluf SW, Erdtmann B (2001) Genomic instability in Down syndrome and Fanconi anemia assessed by micronucleus analysis and single-cell gel electrophoresis. Cancer Genet Cytogenet 124:71-75.
16. McArt DG, McKerr G, Howard CV, et al. (2009). Modelling the comet assay. Biochem Soc Trans 37:914-917.
17. McKelvey-Martin VJ, Melia N, Walsh IK, et al. (1997) Two potential clinical applications of the alkaline single-cell gel electrophoresis assay: (1) human bladder washings and transitional cell carcinoma of the bladder and (2) human sperm and male infertility. Mutat Res 375:93-104.
18. Moller P (2005) Genotoxicity of environmental agents assessed by the alkaline comet assay. Basic Clin Pharmacol Toxicol 1: 1-42.
19. Ohnishi T, Mori E, Takahashi A (2009) DNA double-strand breaks: their production, recognition, and repair in eukaryotes. Mutat Res 669:8-12.
20. Olliver JR, Hardie LJ, Dexter S, et al. (2003). DNA damage levels are raised in Barrett's oesophageal mucosa relative to the squamous epithelium of the oesophagus. Biomarkers 8:509-521.
21. Olliver JR, Hardie LJ, Gong Y, et al. (2005) Risk factors, DNA damage, and disease progression in Barrett's esophagus. Cancer Epidemiol Biomarkers Prev 14:620-625.
22. Olyaee M, Sontag S, Salman W, et al. (1995) Mucosal reactive oxygen species production in esophagitis and Barrett'sesophagus. Gut 37:168-173.
23. Pool-Zobel BL, Guigas C, Klein RG (1993) Assessment of genotoxic effects of lindane. Food Chem Toxicol 31:271-283.
24. Powell SN, Bindra RS (2009) Targeting the DNA damage response for cancer therapy. DNA Repair (Amst) 8:1153-1165.
25. Rajeswari N, Ahuja YR, Malani U, et al. (2000) Risk assessment in first-degree relatives of breast cancer patients using the alkaline comet assay. Carcinogenesis 21:557-561.
26. Rajput S, Wilber A (2010) Roles of inflammation in cancer initiation, progression, and metastasis. Front Biosci (Sch Ed) 2:176-183.
27. Ribeiro ML, Priolli DG, Miranda DD, et al. (2008) Analysis of oxidative DNA damage in patients with colorectal cancer. Clin Colorectal Cancer 7:267-272.
28. Santella RM (1997) DNA damage as an intermediate biomarker in intervention studies. Biomarkers Intervention Stud 216:166-171.
29. Saran R, Vijayshree B, Ahuja YR (1999) Alkaline single cell gel electrophoresis assay II: basic and applied aspects. Cytol Genet 34:63-67.
30. Schwartz JL, Rotmensch J, Giovanazzi S, et al. (1988) Faster repair of DNA double strand breaks in radiosensitive human tumor cells. Int J Radiat Oncol Biol Phys 15:907-912.
31. Shao L, Lin J, Huang M, et al. (2005) Predictors of esophageal cancer risk: assessment of susceptibility to DNA damage using comet assay. Genes Chromosomes Cancer 44:415-422.
32. Singh NP (2000) A simple method for accurate estimation of apoptotic cells. Exp Cell Res 256:328-337.
33. Singh NP, McCoy MT, Tice RR, et al. (1998) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191.
34. Udumudi A, Jaiswal M, Rajeswari N, et al. (1998) Risk assessment in cervical dysplasia patients by single cell gel electrophoresis assay: a study of DNA damage and repair. Mutat Res 412:195-205.
35. Vasavi M, Bhavani V, Kusumanjali G, et al. (2006) DNA methylation in esophageal pathologies including cancer: special reference to hMLH1 gene promoter status. Tumori 92:155-162.
36. Vasavi M, Shivani P, Hemakumar MR, et al. (2007) Chromosome 11 aneusomy in esophageal cancers and precancerous lesionsan early event in neoplastic transformation: An interphase fluorescence in situ hybridization study from south India. World J Gastroenterol 13:503-508.
37. Wu HJ, Chi CW, Liu TY (2005) Effects of pH on nicotine-induced DNA damage and oxidative stress. J Toxicol Environ Health 68:1511-1523.