Limitations of beam damage in electron spectroscopic tomography of embedded cells

Journal of Microscopy

Volumn 239, Issue 3, 2010, Pages 223-232

  Aronova, M A ^a   Sousa, A A ^a   Zhang, G ^a   Leapman, R D ^a  

^a NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING   (United States)

Author keywords

Biological applications; cell nucleus; EFTEM; electron tomography; elemental mapping; energy filtering transmission electron microscopy; nitrogen; phosphorus

Indexed keywords

BIOMOLECULES; CYTOLOGY; ELECTRONS; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; MAPPING; NITROGEN; NUCLEIC ACIDS; OXYGEN; PROTEINS;

BEAM DAMAGE; BIOLOGICAL APPLICATIONS; CELL NUCLEUS; ELECTRON TOMOGRAPHY; ELEMENTAL MAPPING; EMBEDDED CELLS; ENERGY FILTERING; ENERGY FILTERING TRANSMISSION ELECTRON MICROSCOPE; ENERGY-FILTERING TRANSMISSION ELECTRON MICROSCOPIES; TRANSMISSION ELECTRON;

PHOSPHORUS;

NITROGEN; NUCLEIC ACID; OXYGEN; PHOSPHORUS;

ANIMAL CELL; ARTICLE; ARTIFACT; CAENORHABDITIS ELEGANS; CELLULAR DISTRIBUTION; ELECTRON BEAM TOMOGRAPHY; ENERGY FILTERED TRANSMISSION ELECTRON MICROSCOPY; FEASIBILITY STUDY; IMAGE RECONSTRUCTION; MASS; MORPHOLOGY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; THYMOCYTE;

CAENORHABDITIS ELEGANS;

EID: 77955733191     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2010.03376.x     Document Type: Article

References (35)

1. Aronova, M.A., Kim, Y.C., Harmon, R., Sousa, A.A., Zhang, G. & Leapman, R.D. (2007a) Three-dimensional elemental mapping of phosphorus by quantitative electron spectroscopic tomography (QuEST). J. Struct. Biol. 160, 35 48.
2. Aronova, M.A., Kim, Y.C., Zhang, G. & Leapman, R.D. (2007b) Quantification and thickness correction of EFTEM phosphorus maps. Ultramicroscopy 107, 232 244.
3. Baumeister, W. (2004) Mapping molecular landscapes inside cells. Biol. Chem. 385, 865 872.
4. Bazett-Jones, D.P. & Hendzel, M.J. (1999) Electron spectroscopic imaging of chromatin. Methods Enzymol. 17, 188 200.
5. Bazett-Jones, D.P., Hendzel, M.J. & Kruhlak, M.J. (1999) Stoichiometric analysis of protein- and nucleic acid-based structures in the cell nucleus. Micron 30, 151 157.
6. Bazett-Jones, D.P., Locklear, L. & Rattner, J.B. (1988) Electron spectroscopic imaging of DNA. J. Ultrastruc. Mol. Struc. Res. 99, 48 58.
7. Braunfeld, M.B., Koster, A.J., Sedat, J.W. & Agard D.A. (1994) Cryo automated electron tomography: towards high resolution reconstructions of plastic-embedded structures. J. Micros. 174, 75 84.
8. Egerton, R.F. (1996) Electron Energy Loss Spectroscopy. 2nd edn. Plenum
9. Egerton, R.F., Li, P. & Malac, M. (2004) Radiation damage in the TEM and SEM. Micron 35, 399 409.
10. Egerton, R.F., Wang, F. & Crozier, P.A. (2006) Beam-induced damage to thin specimens in an intense electron probe. Microsc. Microanal. 12, 65 71.
11. Frank, J. (1992) Electron Tomography: Three-Dimensional Imaging with the Transmission Electron Microscope. Plenum
12. Frank, J. (2006) Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell. 2nd edn. Springer
13. Grimm R., Singh J., Rachel R., Typke D., Zillig, W. & Baumeister W. (1998) Electron tomography of ice-embedded prokaryotic cells. Biophys. J. 74, 1031 1042.
14. Grünewald, K., Desai, P., Winkler, D.C., Heymann, J.B., Belnap, D.M., Baumeister, W. & Steven, A.C. (2003) Three-dimensional structure of herpes simplex virus from cryo-electron tomography. Science 302, 1396 1398.
15. Haking, A., Troester, H., Richter, K., Crucifix, C., Spring, H. & Trendelenburg, F. (1999) An approach to an objective background subtraction for elemental mapping with core-edges down to 50 eV: description, evaluation and application. Ultramicroscopy 80, 163 182.
16. Harmon, R.T., Meyer, S. & Hunt, J.A. (2005) Software for EFTEM Tomography Incorporated into Digital MicrographGMS. Gatan, Inc.
17. Hsieh, C.-E., Marko, M., Leith, A., Mannella, C.A. & Frank, J. (2006) Towards high-resolution three-dimensional imaging of native mammalian tissue: electron tomography of frozen-hydrated rat liver sections. J. Struct. Biol. 153, 1 13.
18. Isaacson, M.S. (1977) Specimen damage in the electron microscope. Principles and Techniques of Electron Microscopy VII (ed. by M.A. Hayat pp. 1 78, van Nostrand Reinhold
19. Koster, A.J., Grimm, R., Typke, D., Hegerl, R., Stoschek, A., Walz, J. & Baumeister, W. (1997) Prespectives of molecular and cellular electron tomography. J. Struct. Biol. 120, 276 308.
20. Kremer, J.S., Mastronarde, D.N. & McIntosh, J.R. (1996) Computer visualization of three dimensional image data using IMOD. J. Struct. Biol. 116, 71 76.
21. Leapman, R.D. (1986) STEM elemental mapping by electron energy-loss spectroscopy. Ann. N.Y. Acad. Sci. 483, 326 338.
22. Leapman, R.D. (2003) Detecting single atoms of calcium and iron in biological structures by electron energy-loss spectrum-imaging. J. Microsc. 210 (1 5 15.
23. Leapman, R.D. & Aronova, M.A. (2007) Localizing specific elements bound to macromolecules by EFTEM. Meth. Cell Biol. 79, 593 613.
24. Leapman, R.D., Fiori, C. E. & Swyt, C.R. (1984) Mass thickness determination by electron energy loss for quantitative X-ray microanalysis in biology. J. Microsc. 133, 239 253.
25. Leapman, R.D., Jarnik, M. & Steven, A.C. (1997) Spatial distributions of sulfur-rich proteins in cornifying epithelia. J. Struct. Biol. 120, 168 179.
26. Leapman, R.D., Kocsis, E., Zhang, G., Talbot, T.L. & Laquerriere, P. (2004) Three-dimensional distributions of elements in biological samples by energy-filtered electron tomography. Ultramicroscopy 100, 115 125.
27. Leapman, R.D. & Ornberg, R.L. (1988) Quantitative electron energy loss spectroscopy in biology. Ultramicroscopy 24 (2-3 251 268.
28. Leapman, R.D. & Rizzo, N.W. (1999) Towards single atom analysis of biological structures. Ultramicroscopy 78, 251 268.
29. Mastronarde, D.N. (1997) Dual axis tomography: an approach with alignment methods that preserve resolution. J. Struct. Biol. 120, 343 352.
30. McEwen, B.F., Downing, K.H. & Glaeser, R.M. (1995) The relevance of dose-fractionation in tomography of radiation-sensitive specimens. Ultramicroscopy 60 (3 357 373.
31. McIntosh, R., Nicastro, D. & Mastronarde, D. (2005) New views of cells in 3D: and introduction to electron tomography. Trends Cell Biol. 15 (1 1 9.
32. Ottensmeyer, F.P. (1984) Electron spectroscopic imaging: parallel energy filtering and microanalysis in the fixed-beam electron microscope. J. Ultrastruct. Res. 88, 121 134.
33. Ottensmeyer, F.P. & Andrew, J.W. (1980) High-resolution microanalysis of biological specimens by electron energy loss spectroscopy and by electron spectroscopic imaging. J. Ultrastruct. Res. 72, 336 348.
34. Reimer, L. (1995) Energy-Filtering Transmission Electron Microscopy. Springer
35. Subramaniam, S. (2005) Bridging the imaging gap: Visualizing subcellular architecture with electron tomography. Curr. Opin. Microbiol. 8, 316 322.