Production, characterization, and crystallization of truncated forms of pneumococcal surface protein A from Escherichia coli

Protein Expression and Purification

Volumn 20, Issue 3, 2000, Pages 379-388

  Lamani, Ejvis ^a   McPherson, David T ^a   Hollingshead, Susan K ^a   Jedrzejas, Mark J ^a  

^a University of Alabama at Birmingham   (United States)

Author keywords

Pathogen; Streptococcus pneumoniae; Structure and function; Vaccine

Indexed keywords

BACTERIAL INFECTION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENETIC STRAIN; INFECTIVITY; MOUSE; PNEUMOCOCCUS SURFACE PROTEIN A; POLYMERASE CHAIN REACTION; PROTEIN PURIFICATION; RNA TRANSLATION; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PNEUMOCOCCUS; STREPTOCOCCUS PNEUMONIAE;

EID: 0033668107     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1320     Document Type: Article

References (35)

1. Streptococcus pneumoniae
2. Pathogenesis of pneumococcal pneumonia
3. Epidemiologic studies of Streptococcus pneumoniae in infants: Acquisition, carriage, and infection during the first 24 months of life
4. Infections caused by Streptococcus pneumoniae: Clinical spectrum, pathogenesis, immunity, and treatment
5. Antimicrobial resistance of Streptococcus pneumoniae in the United States, The Pneumococcal Surveillance Working Group
6. A review of antibiotic resistance patterns of Streptococcus pneumoniae in Europe
7. Bumps on the vaccine road
8. PspA, a surface protein of Streptococcus pneumoniae, is capable of eliciting protection against pneumococci of more than one capsular type
9. Monoclonal antibodies against protease-sensitive pneumococcal antigens can protect mice from fatal infection with Streptococcus pneumoniae
10. Use of insertional inactivation to facilitate studies of biological properties of pneumococcal surface protein A (PspA)
11. Variation in the molecular weight of PspA (pneumococcal surface protein A) among Streptococcus pneumoniae
12. Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular serotypes of Streptococcus pneumoniae
13. Novel surface attachment mechanism of the Streptococcus pneumoniae protein PspA
14. Structural properties and evolutionary relationships of PspA, a surface protein of Streptococcus pneumoniae, as revealed by sequence analysis
15. Production and characterization of pneumococcal surface protein A
16. PspA and PspC: Their potential for use as pneumococcal vaccines
17. Characterization of the Erwinia carotovora pelB gene and its product pectate lyase
18. Cellular location affects protein stability in Escherichia coli
19. Strategies for achieving high-level expression of genes in Escherichia coli
20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
21. Preparation of selenomethionyl proteins for phase determination
22. Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): A vehicle for direct determination of three-dimensional structure
23. How to measure and predict the molar absorption coefficient of a protein
24. Processing of X-ray diffraction data collected in oscillation mode
25. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding
26. The SWISS-PROT protein sequence data bank
27. Circular dichroic analysis of protein conformation: Inclusion of the β-turns
28. Escherichia coli secretion of an active chimeric antibody fragment
29. Solvent content of protein crystals
30. Phase determination from multiwavelength anomalous diffraction measurements
31. The crystal structure of a five-stranded coiled coil in COMP: A prototype ion channel
32. Crystallization and preliminary crystallographic study of the pentamerizing domain from cartilage oligomeric matrix protein: A five-stranded alpha-helical bundle