Cell cycle characteristics and changes in membrane potential during growth of Escherichia coli as determined by a cyanine fluorescent dye and flow cytometry

Journal of Microbiological Methods

Volumn 25, Issue 1, 1996, Pages 79-86

  Monfort, Patrick ^a   Baleux, Bernard ^a  

^a UNIVERSITÉ MONTPELLIER 2   (France)

Author keywords

cell cycle; DiOC6(3); Escherichia coli; flow cytometry; Hoechst 33342; membrane potential

Indexed keywords

CYANIDIN 3,5 DIGLUCOSIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; CELL CYCLE; CONTROLLED STUDY; DNA REPLICATION; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; MEMBRANE POTENTIAL; NONHUMAN; PRIORITY JOURNAL; STAINING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0029895102     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/0167-7012(95)00089-5     Document Type: Article

References (28)

1. Fouchet, P., Jayat, C., Héchard, Y., Ratinaud, M.H. and Frelat, G. (1993) Recent advances of flow cytometry in fundamental and applied microbiology. Biol. Cell. 78, 95-109.
2. Laplace-Builhé, C., Hahna, K., Hunger, W., Tirilly, Y. and Drocourt, J.L. (1993) Application of flow cytometry to rapid microbial analysis in food and drinks industries. Biol. Cell. 78, 123-128.
3. Monfort, P. and Baleux, B. (1992) Comparison of flow cytometry and epifluorescence microscopy for counting bacteria in aquatic ecosystems. Cytometry 13, 188-192.
4. Monfort, P. and Baleux, B. (1994) Effects of environmental factors present in the St. Lawrence Estuary (Quebec, Canada) on experimental survival of Salmonella salamae as determined by flow cytometry. Can. J. Microbiol. 40, 712-719.
5. Troussellier, M., Courties, C. and Vaquer, A. (1993) Recent applications of flow cytometry in aquatic microbial ecology. Biol. Cell. 78, 111-121.
6. Boye, E. and Løbner-Olesen, A. (1991) Bacterial growth control studied by flow cytometry. Res. Microbiol. 142, 132-135.
7. Boye, E. and Steen, H. (1993) The physical and biological basis for flow cytometry of Escherichia coli. In: Flow Cytometry in Microbiology (Lloyd, D., ed.), pp. 11-25, Springer-Verlag, London.
8. Roszak, D.B. and Colwell, R.R. (1987) Metabolic activity of bacterial cells enumerated by direct viable count. Appl. Environ. Microbiol. 53, 2889-2983.
9. Rodriguez, G.G., Phipps, D., Ishiguro, K. and Ridgway, H.F. (1992) Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58, 1801-1808.
10. Diaper, J.P., Tither, K. and Edwards, C. (1992) Rapid assessment of bacterial viability by flow cytometry. Appl. Microbiol. Biotechnol. 38, 268-272.
11. Kaprelyants, A.S. and Kell, D.B. (1992) Rapid assessment of bacterial viability by rhodamine 123 and flow cytometry. J. Appl. Bacteriol. 72, 410-422.
12. Shapiro, H.M. (1995) Practical Flow Cytometry, 3rd edn., Wiley-Liss, New York.
13. Matsuyama, T. (1984) Staining of living bacteria with rhodamine 123. FEMS Microbiol. Lett. 21, 153-157.
14. Yu, F.P. and McFeters, G.A. (1994) Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes. J. Microbiol. Methods 20, 1-10.
15. Yu, F.P. and McFeters, G.A. (1994) Physiological responses of bacteria in biofilms to disinfection. Appl. Environ. Microbiol. 60, 2462-2466.
16. Mason, D., Allman, R. and Lloyd, D. (1993) Use of membrane potential sensitive dyes with bacteria. In: Flow Cytometry in Microbiology (Lloyd, D., ed.), pp. 67-81, Springer-Verlag, London.
17. Darzynkiewicz, Z., Staiano-Coico, L. and Melamed, M.R. (1981) Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation. Proc. Natl. Acad. Sci. USA 78, 2383-2387.
18. Leprat, P., Ratinaud, M.H., Maftah, A., Petit, J.M. and Julien, R. (1990) Use of nonyl acridine orange and rhodamine 123 to follow biosynthesis and functional assembly of mitochondrial membrane during L1210 cell cycle. Exp, Cell Res. 186, 130-137.
19. Ratinaud, M.H. and Revidon, S. (1996) The cyanine fluorescent dye and flow cytometry: a suitable method to assess functional state of the Listeria membrane. J. Microbiol. Methods 25, 71-77.
20. Maftah, A., Renault, D., Vignoles, C., Héchard, Y., Bressollier, P., Ratinaud, M.H., Cenatiempo, Y. and Julien, R. (1993) Membrane permeabilization of Listeria monocytogenes and mitochondria by the bacteriocin mesentericin Y105. Appl. Environ. Microbiol. 175, 3232-3235.
21. Mason, D.J., Lopez-Amoros, R., Allman, R., Stark, J.M. and Lloyd, D. (1995) The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria. J. Appl. Bacteriol. 78, 309-315.
22. Pinder, A.C., Purdy, P.W., Poulter, S.A.G. and Clark, D.C. (1990) Validation of flow cytometry for rapid enumeration of bacterial concentrations in pure cultures. J. Appl. Bacteriol. 69, 92-100.
23. Jayat, C. and Ratinaud, M.H. (1993) Cell cycle analysis by flow cytometry: principles and applications. Biol. Cell. 78, 15-25.
24. Cooper, S. (1991) Bacterial Growth and Division. Biochemistry and Regulation of Prokaryotic and Eukaryotic Division Cycles. Academic Press, London.
25. Lopez-Amoros, R., Comas, J., Carulla, C. and Vives-Rego, J. (1994) Variations in flow cytometric forward scatter signals and cell size in batch cultures of Escherichia coli. FEMS Microbiol. Lett. 117, 225-230.
26. Ordonez, J.V. and Wehman, N.M. (1993) Rapid flow cytometric antibiotic susceptibility assay for Staphylococcus aureus. Cytometry 14, 811-818.
27. Mason, D.J., Allman, R., Stark, J.M. and Lloyd, D. (1994) Rapid estimation of bacterial antibiotic susceptibility with flow cytometry. J. Microsc., 176, 8-16.
28. Gant, V.A., Warnes, G., Phillips, I. and Savidge, G.F. (1993) The application of flow cytometry to the study of bacterial responses to antibiotics. J. Med. Microbiol. 39, 147-154.