DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry

American Journal of Clinical Pathology

Volumn 129, Issue 5, 2008, Pages 706-713

  Allan, Robert W ^a   Ansari Lari, M A ^b   Jordan, Sandra ^b  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^b MEMORIAL REGIONAL HOSPITAL   (United States)

Author keywords

Bone marrow aspirate; DRAQ5; Erythroid precursors; Flow cytometry; Hematolymphoid neoplasms; Leukocytes

Indexed keywords

ANTHRAQUINONE DERIVATIVE; CD45 ANTIGEN; CD71 ANTIGEN; DNA BINDING ANTHRAQUINONE; DIAGNOSTIC AGENT; LEUKOCYTE ANTIGEN; TRANSFERRIN RECEPTOR;

ARTICLE; BONE MARROW BIOPSY; CELL LOSS; CLINICAL ARTICLE; CLINICAL EVALUATION; DIAGNOSTIC PROCEDURE; ERYTHROID PRECURSOR CELL; FLOW CYTOMETRY; HEMOLYSIS; HUMAN; HUMAN TISSUE; LABORATORY TEST; MORPHOLOGY; PRIORITY JOURNAL; BLOOD DISEASE; BONE MARROW; BONE MARROW EXAMINATION; FEMALE; MALE; METABOLISM; METHODOLOGY; NEEDLE BIOPSY; PATHOLOGY; SENSITIVITY AND SPECIFICITY;

ANTHRAQUINONES; ANTIGENS, CD; ANTIGENS, CD45; BIOPSY, NEEDLE; BONE MARROW; BONE MARROW EXAMINATION; FEMALE; FLOW CYTOMETRY; HEMATOLOGIC NEOPLASMS; HUMANS; MALE; RECEPTORS, TRANSFERRIN; SENSITIVITY AND SPECIFICITY;

EID: 44849098715     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1309/D2M6Q7BYHWAM1FYQ     Document Type: Article

References (11)

1. Nguyen D, Diamond LW, Braylan RC. Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation. Totowa, NJ: Humana Press; 2003:136.
2. Borowitz MJ, Guenther KL, Shults KE, et al. Immunophenotyping of acute leukemia by flow cytometric analysis: use of CD45 and right angle side scatter to gate on leukemic blasts in three color analysis. Am J Clin Pathol. 1993;100:534-540.
3. Carey JL. Immunophenotyping in diagnosis and prognosis of mature lymphoid leukemias and lymphomas. In: Keren DF, McCoy JP, Carey JL, eds. Flow Cytometry in Clinical Diagnosis. 3rd ed. Chicago, IL: ASCP Press; 2001:227-338.
4. Smith PJ, Wiltshire M, Davies S, et al. A novel cell permeant and far red fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry. J Immunol Methods. 1999;229:131-139.
5. Terstappen LWMM, Loken MR. Five dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry. 1988;9:548-556.
6. McCarthy DA, Macey MG. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993;163:155-160.
7. Rainer RO, Hodges L, Seltzer GT. CD45 gating correlates with bone marrow differential. Cytometry. 1995;22:139-145.
8. Stetler-Stevenson M, Arthur DC, Jabbour N. Diagnostic utility of immunophenotyping in myelodysplastic syndrome. Blood. 2001;98:979-987.
9. van Schravendijk MR, Handunnetti SM, Barnwell JW, et al. Normal human erythrocytes express CD36, an adhesion molecule of monocytes, platelets, and endothelial cells. Blood. 1992;80:2105-2114.
10. Ervasti M, Matinlauri I, Punnonen K. Quantitative flow cytometric analysis of transferrin receptor expression on reticulocytes. Clin Chim Acta. 2007;383:153-157.
11. Houwen B. The differential cell count. Lab Hematol. 2001;7:89-100.