Site-directed mutagenesis of disulfide bridges in Aspergillus niger NRRL 3135 phytase (PhyA), their expression in Pichia pastoris and catalytic characterization

Applied Microbiology and Biotechnology

Volumn 87, Issue 4, 2010, Pages 1367-1372

  Mullaney, Edward J ^a   Locovare, Heather ^a   Sethumadhavan, Kandan ^a   Boone, Stephanie ^a   Lei, Xin Gen ^b   Ullah, Abul H J ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

^b Department of Obstetrics Gynecology   (United States)

Author keywords

Aspergillus niger; Disulfide bridge; Histidine acid phosphatase; Phytase

Indexed keywords

ACID PHOSPHATASE; ACTIVITY PROFILE; ASPERGILLUS NIGER; CATALYTIC ACTIVITY; CATALYTIC CHARACTERIZATION; CATALYTIC PROPERTIES; DISULFIDE BRIDGE; EXPRESSION SYSTEM; MUTANT PHYTASE; NIGER; OPTIMUM TEMPERATURE; PHYA GENE; PHYTASES; PICHIA PASTORIS; RELATIVE IMPORTANCE; SITE DIRECTED MUTAGENESIS; TURNOVER NUMBER; WILD TYPES;

ACIDS; MUTAGENESIS;

PHOSPHATASES;

CYSTEINE; DISULFIDE; PHYTASE; FUNGAL PROTEIN;

ENZYME ACTIVITY; FUNGUS; GENE EXPRESSION; GENETIC ANALYSIS; MUTATION; PH; TEMPERATURE PROFILE;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIGER; CATALYSIS; ENZYME ACTIVITY; GENE EXPRESSION SYSTEM; GENE MUTATION; KINETICS; LOW TEMPERATURE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; WILD TYPE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR GENETICS; PICHIA;

6-PHYTASE; AMINO ACID SEQUENCE; ASPERGILLUS NIGER; CATALYSIS; DISULFIDES; ENZYME STABILITY; FUNGAL PROTEINS; GENE EXPRESSION; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PICHIA;

ASPERGILLUS NIGER; PICHIA; PICHIA PASTORIS;

EID: 77955554589     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2542-2     Document Type: Article

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