Monitoring of unfolding and refolding in fungal phytase (phyA) by dynamic light scattering

Biochemical and Biophysical Research Communications

Volumn 327, Issue 4, 2005, Pages 993-998

  Ullah, Abul H J ^a   Sethumadhavan, Kandan ^a   Mullaney, Edward J ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Cysteine; Dynamic light scattering; Guanidinium chloride; Histidine acid phosphatase; Phytase; Protein refolding; Protein unfolding

Indexed keywords

FUNGAL ENZYME; GUANIDINE DERIVATIVE; PHOSPHINE DERIVATIVE; PHYTASE; REAGENT; THIOL DERIVATIVE;

ARTICLE; CATALYSIS; DIALYSIS; DISULFIDE BOND; ENZYME DENATURATION; HYDRODYNAMICS; LIGHT SCATTERING; PRIORITY JOURNAL; PROTEIN FOLDING; TEMPERATURE;

6-PHYTASE; AMINO ACID SEQUENCE; ASPERGILLUS NIGER; DISULFIDES; HYDROGEN; LIGHT; MERCAPTOETHANOL; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PHOSPHINES; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN RENATURATION; SCATTERING, RADIATION; TEMPERATURE;

EID: 12344274329     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.12.111     Document Type: Article

References (13)

1. A.H.J. Ullah, and D.M. Gibson Extracellular phytase (E.C.3.1.3.8) from Aspergillus ficuum J. Prep. Biochem. 17 1987 63 91
2. R.J. Wodzinski, and A.H.J. Ullah Phytase Adv. Appl. Microbiol. 42 1996 263 302
3. E.J. Mullaney, D.M. Gibson, and A.H.J. Ullah Positive identification of a lambda gt11 clone containing a region of fungal phytase gene by immunoprobe and sequence verification Appl. Microbiol. Biotechnol. 35 1991 611 614
4. A.H.J. Ullah, B.J. Cummins, and H.C. Dischinger Jr. Cyclohexanedione modification of arginine at the active site Biochem. Biophys. Res. Commun. 178 1991 45 53
5. A.H.J. Ullah, and H.C. Dischinger Jr. Aspergillus ficuum phytase: complete primary structure elucidation by chemical sequencing Biochem. Biophys. Res. Commun. 192 1993 747 753
6. E.J. Mullaney, C.B. Daly, and A.H.J. Ullah Advances in phytase research Adv. Appl. Microbiol. 47 2000 157 199
7. D.F. Kostrewa, F. Gruninger-Leitch, A. D'Arcy, C. Broger, D. Mitchell, and A.P.G.M. van Loon Crystal structure of phytase from Aspergillus ficuum at 2.5 Å resolution Nature Struct. Biol. 4 1997 185 190
8. E.J. Mullaney, C.B. Daly, T. Kim, J.M. Porres, X.G. Lei, K. Sethumadhavan, and A.H.J. Ullah Site-directed mutagenesis of Aspergillus niger NRRL 3135 phytase at residue 300 to enhance catalysis at pH 4.0 Biochem. Biophys. Res. Commun. 297 2002 1016 1020
9. M.A. Mallin Impact of industrial animal production on rivers and estuaries Am. Sci. 88 2000 26 37
10. A.H.J. Ullah, and K. Sethumadhavan Phy A gene product of Aspergillus ficuum and Peniophora lycii produces dissimilar phytases Biochem. Biophys. Res. Commun. 303 2003 463 468
11. A.H.J. Ullah, and E.J. Mullaney Disulfide bonds are necessary for structure and activity in Aspergillus ficuum phytase Biochem. Biophys. Res. Commun. 227 1996 311 317
12. X.Y. Wang, F.G. Meng, and X.Y. Wang The role of disulfide bonds in the conformational stability and catalytic activity of phytase Biochem. Cell Biol. 82 2004 329 334
13. X.Y. Wang, F.G. Meng, and X.Y. Wang Unfolding and inactivation during thermal denaturation of an enzyme that exhibits phytase and acid phosphatase activities Int. J. Biochem. Cell Biol. 36 2004 447 459