Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surfaces

European Cells and Materials

Volumn 19, Issue , 2010, Pages 228-241

  Docheva, D ^a   Padula, D ^a,b,c   Popov, C ^a   Weishaupt, P ^c   Pragert M ^a   Miosge, N ^d   Hickel, R ^c   Bocker W ^a   Clausen Schaumann, H ^b,c   Schieker, M ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b MUNICH UNIVERSITY OF APPLIED SCIENCES   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

^d UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Biocompatibility; Osteogenic differentiation; Periodontal ligament; Progenitor cell; Scaffold; Surface topography; Titanium

Indexed keywords

TELOMERASE; TERT PROTEIN, HUMAN; TISSUE SCAFFOLD; TITANIUM;

ARTICLE; CELL DIFFERENTIATION; CELL LINE; CYTOLOGY; GENE TRANSFER; GENETICS; HUMAN; LENTIVIRINAE; MATERIALS TESTING; METHODOLOGY; PERIODONTAL LIGAMENT; PERIODONTITIS; SURFACE PROPERTY; TISSUE ENGINEERING;

CELL DIFFERENTIATION; CELL LINE, TRANSFORMED; GENE TRANSFER TECHNIQUES; HUMANS; LENTIVIRUS; MATERIALS TESTING; PERIODONTAL LIGAMENT; PERIODONTITIS; RESEARCH DESIGN; SURFACE PROPERTIES; TELOMERASE; TISSUE ENGINEERING; TISSUE SCAFFOLDS; TITANIUM;

EID: 77955483188     PISSN: 14732262     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Bluteau G, Luder HU, De Bari C, Mitsiadis TA (2008) Stem cells for tooth engineering. Eur Cell Mater 16: 1-9.
2. Böcker W, Rossmann O, Docheva D, Malterer G, Mutschler W, Schieker M (2007) Quantitative polymerase chain reaction as a reliable method to determine functional lentiviral titer after ex vivo gene transfer in human mesenchymal stem cells. J Gene Med 9: 585-595.
3. Böcker W, Yin Z, Drosse I, Haasters F, Rossmann O, Wierer M, Popov C, Locher M, Mutschler W, Docheva D, Schieker M (2008) Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer. J Cell Mol Med 12: 1347-1359.
4. Boyan BD, Lossdorfer S, Wang L, Zhao G, Lohmann CH, Cochran DL, Schwartz Z (2003) Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies. Eur Cell Mater 6: 22-27.
5. Buser D (2001) Titanium for dental application. In: Brunette DM, Tengvall P, Textor M (eds) Titanium in medicine: material science, surface science, engineering, biological responses and medical applications. Springer, Berlin, pp 875-888
6. Docheva D, Hunziker EB, Fassler R, Brandau O (2005) Tenomodulin is necessary for tenocyte proliferation and tendon maturation. Mol Cell Biol 25: 699-705.
7. Docheva D, Popov C, Mutschler W, Schieker M (2007) Human mesenchymal stem cells in contact with their environment: surface characteristics and the integrin system. J Cell Mol Med 11: 21-38.
8. Docheva D, Haasters F, Schieker M (2008) Mesenchymal stem cells and their cell surface receptors. Curr Rheumatol Rev 4: 155-160.
9. Fujii S, Maeda H, Wada N, Tomokiyo A, Saito M, Akamine A (2008) Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo. J Cell Physiol 215: 743-749.
10. Fujita T, Otsuka-Tanaka Y, Tahara H, Ide T, Abiko Y, Mega J (2005) Establishment of immortalized clonal cells derived from periodontal ligament cells by induction of the hTERT gene. J Oral Sci 47: 177-184.
11. Hacking SA, Harvey E, Roughley P, Tanzer M, Bobyn J (2008) The response of mineralizing culture systems to microtextured and polished titanium surfaces. J Orthop Res 26: 1347-1354.
12. Huang GT, Shagramanova K, Chan SW (2006) Formation of odontoblast-like cells from cultured human dental pulp cells on dentin in vitro. J Endod 32: 1066-1073.
13. Itaya T, Kagami H, Okada K, Yamawaki A, Narita Y, Inoue M, Sumita Y, Ueda M (2009) Characteristic changes of periodontal ligament-derived cells during passage. J Periodontal Res 44: 425-433.
14. Ivanovski S, Gronthos S, Shi S, Bartold PM (2006) Stem cells in the periodontal ligament. Oral Dis 12: 358-363.
15. Karageorgiou V, Kaplan D (2005) Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26: 5474-5491.
16. Kim MJ, Kim CW, Lim YJ, Heo SJ (2006) Microrough titanium surface affects biologic response in MG63 osteoblast-like cells. J Biomed Mater Res A 79: 1023-1032.
17. Kohn DB (2007) Lentiviral vectors ready for prime-time. Nat Biotechnol 25: 65-66.
18. Kramer PR, Janikkeith A, Cai Z, Ma S, Watanabe I (2009) Integrin mediated attachment of periodontal ligament to titanium surfaces. Dent Mater 25: 877-883.
19. Levine BL, Humeau LM, Boyer J, MacGregor RR, Rebello T, Lu X, Binder GK, Slepushkin V, Lemiale F, Mascola JR, Bushman FD, Dropulic B, June CH (2006) Gene transfer in humans using a conditionally replicating lentiviral vector. Proc Natl Acad Sci U S A 103: 17372-17377.
20. Lumbikanonda N, Sammons R (2001) Bone cell attachment to dental implants of different surface characteristics. Int J Oral Maxillofac Implants 16: 627-636.
21. Mangano C, De Rosa A, Desiderio V, d'Aquino R, Piattelli A, De Francesco F, Tirino V, Mangano F, Papaccio G (2010) The osteoblastic differentiation of dental pulp stem cells and bone formation on different titanium surface textures. Biomaterials 31: 3543-3551.
22. Oates TW, Maller SC, West J, Steffensen B (2005) Human gingival fibroblast integrin subunit expression on titanium implant surfaces. J Periodontol 76: 1743-1750.
23. Parlar A, Bosshardt DD, Unsal B, Cetiner D, Haytac C, Lang NP (2005) New formation of periodontal tissues around titanium implants in a novel dentin chamber model. Clin Oral Implants Res 16: 259-267.
24. Pfeifer A, Ikawa M, Dayn Y, Verma IM (2002) Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci U S A 99: 2140-2145.
25. Pi SH, Lee SK, Hwang YS, Choi MG, Lee SK, Kim EC (2007) Differential expression of periodontal ligament-specific markers and osteogenic differentiation in human papilloma virus 16-immortalized human gingival fibroblasts and periodontal ligament cells. J Periodontal Res 42: 104-113.
26. Ricci JL, Grew JC, Alexander H (2008) Connective-tissue responses to defined biomaterial surfaces. I. Growth of rat fibroblast and bone marrow cell colonies on microgrooved substrates. J Biomed Mater Res A 85: 313-325.
27. Rupp F, Scheideler L, Olshanska N, de Wild M, Wieland M, Geis-Gerstorfer J (2006) Enhancing surface free energy and hydrophilicity through chemical modification of microstructured titanium implant surfaces. J Biomed Mater Res A 76: 323-334.
28. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S (2004) Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 364: 149-155.
29. Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S (2005) The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res 8: 191-199.
30. Shibli JA, Grassi S, de Figueiredo LC, Feres M, Marcantonio E Jr, Iezzi G, Piattelli A (2007) Influence of implant surface topography on early osseointegration: a histological study in human jaws. J Biomed Mater Res B Appl Biomater 80: 377-385.
31. Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, Akamine A (2008) Development of a multipotent clonal human periodontal ligament cell line. Differentiation 76: 337-347.
32. Wall I, Donos N, Carlqvist K, Jones F, Brett P (2009) Modified titanium surfaces promote accelerated osteogenic differentiation of mesenchymal stromal cells in vitro. Bone 45: 17-26.