TRNALys3 promoter cassettes that efficiently express RNAi-activating antihepatitis B virus short hairpin RNAs

Biochemical and Biophysical Research Communications

Volumn 398, Issue 4, 2010, Pages 640-646

  Dyer, Victoria ^a   Ely, Abdullah ^a   Bloom, Kristie ^a   Weinberg, Marc ^a   Arbuthnot, Patrick ^a  

^a UNIVERSITY OF THE WITWATERSRAND MEDICAL SCHOOL   (South Africa)

Author keywords

Expression cassette; Hepatitis B virus; Promoter; RNAi; TRNA

Indexed keywords

DNA DIRECTED RNA POLYMERASE III; LYSINE; MICRORNA; SHORT HAIRPIN RNA; TRANSFER RNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIVIRAL ACTIVITY; ARTICLE; CELL CULTURE; CONTROLLED STUDY; GENE EXPRESSION; GENE SILENCING; GENE TARGETING; GROWTH INHIBITION; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; HYDRODYNAMICS; IN SITU HYBRIDIZATION; IN VIVO STUDY; MOUSE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; RNA INTERFERENCE; RNA SEQUENCE; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION; VIRUS REPLICATION; VIRUS STRAIN;

ANIMALS; CELL LINE; GENE THERAPY; GENES, REPORTER; HEPATITIS B; HEPATITIS B VIRUS; HUMANS; MICE; MICRORNAS; PROMOTER REGIONS, GENETIC; RNA INTERFERENCE; RNA, TRANSFER, LYS; VIRUS REPLICATION;

CERCOPITHECINE HERPESVIRUS 1; HEPATITIS B VIRUS; MURINAE;

EID: 77955432320     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.06.122     Document Type: Article

References (23)

1. Kim V.N., Han J., Siomi M.C. Biogenesis of small RNAs in animals. Nat. Rev. Mol. Cell Biol. 2009, 10:126-139.
2. Bogerd H.P., Karnowski H.W., Cai X., Shin J., Pohlers M., Cullen B.R. A mammalian herpesvirus uses noncanonical expression and processing mechanisms to generate viral microRNAs. Mol. Cell 2010, 37:135-142.
3. Berezikov E., Chung W.J., Willis J., Cuppen E., Lai E.C. Mammalian mirtron genes. Mol. Cell 2007, 28:328-336.
4. Ruby J.G., Jan C.H., Bartel D.P. Intronic microRNA precursors that bypass Drosha processing. Nature 2007, 448:83-86.
5. Babiarz J.E., Ruby J.G., Wang Y., Bartel D.P., Blelloch R. Mouse ES cells express endogenous shRNAs, siRNAs, and other microprocessor-independent, Dicer-dependent small RNAs. Genes Dev. 2008, 22:2773-2785.
6. Ely A., Naidoo T., Arbuthnot P. Efficient silencing of gene expression with modular trimeric Pol II expression cassettes comprising microRNA shuttles. Nucleic Acids Res. 2009, 37:e91.
7. Ely A., Naidoo T., Mufamadi S., Crowther C., Arbuthnot P. Expressed anti-HBV primary microRNA shuttles inhibit viral replication efficiently in vitro and in vivo. Mol. Ther. 2008, 16:1105-1112.
8. Liu Y.P., Haasnoot J., ter Brake O., Berkhout B., Konstantinova P. Inhibition of HIV-1 by multiple siRNAs expressed from a single microRNA polycistron. Nucleic Acids Res. 2008, 36:2811-2824.
9. Aagaard L.A., Zhang J., von Eije K.J., Li H., Saetrom P., Amarzguioui M., Rossi J.J. Engineering and optimization of the miR-106b cluster for ectopic expression of multiplexed anti-HIV RNAs. Gene Ther. 2008, 15:1536-1549.
10. Kawasaki H., Taira K. Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells. Nucleic Acids Res. 2003, 31:700-707.
11. Scherer L.J., Frank R., Rossi J.J. Optimization and characterization of tRNA-shRNA expression constructs. Nucleic Acids Res. 2007, 35:2620-2628.
12. Carmona S., Ely A., Crowther C., Moolla N., Salazar F.H., Marion P.L., Ferry N., Weinberg M.S., Arbuthnot P. Effective inhibition of HBV replication in vivo by anti-HBx short hairpin RNAs. Mol. Ther. 2006, 13:411-421.
13. Weinberg M.S., Ely A., Barichievy S., Crowther C., Mufamadi S., Carmona S., Arbuthnot P. Specific inhibition of HBV replication in vitro and in vivo with expressed long hairpin RNA. Mol. Ther. 2007, 15:534-541.
14. Nassal M. The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly. J. Virol. 1992, 66:4107-4116.
15. Passman M., Weinberg M., Kew M., Arbuthnot P. In situ demonstration of inhibitory effects of hammerhead ribozymes that are targeted to the hepatitis Bx sequence in cultured cells. Biochem. Biophys. Res. Commun. 2000, 268:728-733.
16. Chattopadhyay S., Ely A., Bloom K., Weinberg M.S., Arbuthnot P. Inhibition of hepatitis B virus replication with linear DNA sequences expressing antiviral micro-RNA shuttles. Biochem. Biophys. Res. Commun. 2009, 389:484-489.
17. Ebert M.S., Neilson J.R., Sharp P.A. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat. Methods 2007, 4:721-726.
18. Lagos-Quintana M., Rauhut R., Lendeckel W., Tuschl T. Identification of novel genes coding for small expressed RNAs. Science 2001, 294:853-858.
19. Arbuthnot P., Kew M. Hepatitis B virus and hepatocellular carcinoma. Int. J. Exp. Pathol. 2001, 82:77-100.
20. McCaffrey A.P., Nakai H., Pandey K., Huang Z., Salazar F.H., Xu H., Wieland S.F., Marion P.L., Kay M.A. Inhibition of hepatitis B virus in mice by RNA interference. Nat. Biotechnol. 2003, 21:639-644.
21. Shlomai A., Shaul Y. Inhibition of hepatitis B virus expression and replication by RNA interference. Hepatology 2003, 37:764-770.
22. Grimm D., Streetz K.L., Jopling C.L., Storm T.A., Pandey K., Davis C.R., Marion P., Salazar F., Kay M.A. Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 2006, 441:537-541.
23. Weiwei M., Zhenhua X., Feng L., Hang N., Yuyang J. A significant increase of RNAi efficiency in human cells by the CMV enhancer with a tRNAlys promoter. J. Biomed. Biotechnol. 2009, 514287.