Evaluation of suitable reference gene for real-time PCR in human umbilical cord mesenchymal stem cells with long-term in vitro expansion

In Vitro Cellular and Developmental Biology - Animal

Volumn 46, Issue 7, 2010, Pages 595-599

  Han, Zhongchao ^a,b   Wang, Youwei ^a   Han, Zhibo ^b   Yan, Shulin ^a   Mao, Aibin ^a   Wang, Bin ^a   Ren, He ^a   Chi, Ying ^b  

^a AmCellGene Co Ltd   (China)

^b INSTITUTE OF HEMATOLOGY AND BLOOD DISEASES HOSPITAL   (China)

Author keywords

geNorm; Human umbilical cord mesenchymal stem cells; Real time PCR; Reference genes

Indexed keywords

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELL SHAPE; COMPUTER PROGRAM; CYTOLOGY; EVALUATION; GENE; GENE EXPRESSION REGULATION; GENETICS; HUMAN; MESENCHYMAL STEM CELL; METABOLISM; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD; TIME; UMBILICAL CORD;

CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELL SHAPE; CELLS, CULTURED; GENE EXPRESSION REGULATION; GENES; HUMANS; MESENCHYMAL STEM CELLS; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SOFTWARE; TIME FACTORS; UMBILICAL CORD;

EID: 77955427794     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-010-9318-y     Document Type: Article

References (20)

1. CL Andersen JL Jensen TF Orntoft 2004 Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets Cancer Res 64 5245 5250 10.1158/0008-5472.CAN-04-0496 15289330 (Pubitemid 39006544)
2. FP Barry JM Murphy 2004 Mesenchymal stem cells: clinical applications and biological characterization Int J Biochem Cell Biol 36 568 584 10.1016/j.biocel.2003.11.001 15010324 (Pubitemid 38324589)
3. MM Bonab K Alimoghaddam F Talebian SH Ghaffari A Ghavamzadeh B Nikbin 2006 Aging of mesenchymal stem cell in vitro BMC Cell Biol 7 14 10.1186/1471-2121-7-14 16529651
4. K Dheda JF Huggett JS Chang LU Kim SA Bustin MA Johnson GA Rook A Zumla 2005 The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization Anal Biochem 344 141 143 10.1016/j.ab.2005.05.022 16054107 (Pubitemid 41569389)
5. M Dominici K Le Blanc I Mueller I Slaper-Cortenbach F Marini D Krause R Deans A Keating D Prockop E Horwitz 2006 Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement Cytotherapy 8 315 317 10.1080/14653240600855905 16923606 (Pubitemid 44269669)
6. G Elberg D Elberg CJ Logan L Chen MA Turman 2006 Limitations of commonly used internal controls for real-time RT-PCR analysis of renal epithelial-mesenchymal cell transition Nephron Exp Nephrol 102 e113 e122 10.1159/000090070 16340238 (Pubitemid 43289536)
7. JM Fernandes M Mommens O Hagen I Babiak C Solberg 2008 Selection of suitable reference genes for real-time PCR studies of Atlantic halibut development Comp Biochem Physiol B Biochem Mol Biol 150 23 32 10.1016/j.cbpb.2008.01.003 18302990
8. T Fink P Lund L Pilgaard JG Rasmussen M Duroux V Zachar 2008 Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposure BMC Mol Biol 9 98 10.1186/1471-2199-9-98 18976469
9. S Johansson A Fuchs A Okvist M Karimi C Harper T Garrick D Sheedy Y Hurd G Bakalkin TJ Ekstrom 2007 Validation of endogenous controls for quantitative gene expression analysis: application on brain cortices of human chronic alcoholics Brain Res 1132 20 28 10.1016/j.brainres.2006.11.026 17188656 (Pubitemid 46096453)
10. J Kim HM Kang H Kim MR Kim HC Kwon MC Gye SG Kang HS Yang J You 2007 Ex vivo characteristics of human amniotic membrane-derived stem cells Cloning Stem Cells 9 581 594 10.1089/clo.2007.0027 18154518
11. LL Lu YJ Liu SG Yang QJ Zhao X Wang W Gong ZB Han ZS Xu YX Lu D Liu ZZ Chen ZC Han 2006 Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials Haematologica 91 1017 1026 16870554
12. JH Moon JR Lee BC Jee CS Suh SH Kim HJ Lim HK Kim 2008 Successful vitrification of human amnion-derived mesenchymal stem cells Hum Reprod 23 1760 1770 10.1093/humrep/den202 18541648 (Pubitemid 352020021)
13. BW Park YS Hah DR Kim JR Kim JH Byun 2007 Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells Arch Oral Biol 52 983 989 10.1016/j.archoralbio.2007.04.007 17543271 (Pubitemid 47376678)
14. R Perez I Tupac-Yupanqui S Dunner 2008 Evaluation of suitable reference genes for gene expression studies in bovine muscular tissue BMC Mol Biol 9 79 10.1186/1471-2199-9-79 18786244
15. MF Pittenger AM Mackay SC Beck RK Jaiswal R Douglas JD Mosca MA Moorman DW Simonetti S Craig DR Marshak 1999 Multilineage potential of adult human mesenchymal stem cells Science 284 143 147 10.1126/science.284.5411.143 10102814 (Pubitemid 29282067)
16. S Selvey EW Thompson K Matthaei RA Lea MG Irving LR Griffiths 2001 Beta-actin-an unsuitable internal control for RT-PCR Mol Cell Probes 15 307 311 10.1006/mcpr.2001.0376 11735303 (Pubitemid 33105000)
17. J Vandesompele K De Preter F Pattyn B Poppe N Van Roy A De Paepe F Speleman 2002 Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biol 3 RESEARCH0034 10.1186/gb-2002-3-7-research0034 12184808
18. ML Weiss C Anderson S Medicetty KB Seshareddy RJ Weiss I Vander Werff D Troyer KR McIntosh 2008 Immune properties of human umbilical cord Wharton's jelly-derived cells Stem Cells 26 2865 2874 10.1634/stemcells.2007-1028 18703664
19. E Willems I Mateizel C Kemp G Cauffman K Sermon L Leyns 2006 Selection of reference genes in mouse embryos and in differentiating human and mouse ES cells Int J Dev Biol 50 627 635 10.1387/ijdb.052130ew 16892176 (Pubitemid 44544752)
20. J Ylostalo N Bazhanov DJ Prockop 2008 Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies Exp Hematol 36 1390 1402 10.1016/j.exphem.2008.05.003 18619725