Escherichia coli expression, purification and characterization of functional full-length recombinant α2β2γ3 heterotrimeric complex of human AMP-activated protein kinase

Protein Expression and Purification

Volumn 73, Issue 2, 2010, Pages 189-197

  Rajamohan, Francis ^a   Harris, Melissa S ^a   Frisbie, Richard K ^a   Hoth, Lise R ^a   Geoghegan, Kieran F ^a   Valentine, James J ^a   Reyes, Allan R ^a   Landro, James A ^a   Qiu, Xiayang ^a   Kurumbail, Ravi G ^a  

^a PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

AMP activated protein kinase (AMPK); Bacterial expression; Mass spectrometry; Metabolic syndrome; Polycistronic vector; Protein complex; Recombinant kinase

Indexed keywords

6,7 DIHYDRO 4 HYDROXY 3 (2' HYDROXY 1,1' BIPHENYL 4 YL) 6 OXOTHIENO[2,3 B]PYRIDINE 5 CARBONITRILE; ADENOSINE PHOSPHATE; CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE KINASE; CYCLIC AMP DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT IIBETA; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; PROTEIN SERINE THREONINE KINASE; PROTEIN SUBUNIT; PYRONE DERIVATIVE; RECOMBINANT PROTEIN; THIOPHENE DERIVATIVE;

ARTICLE; DRUG EFFECT; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENETICS; HOMEOSTASIS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY;

ADENOSINE MONOPHOSPHATE; AMP-ACTIVATED PROTEIN KINASES; CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE; CATALYTIC DOMAIN; CYCLIC AMP-DEPENDENT PROTEIN KINASE RIIBETA SUBUNIT; ENZYME ACTIVATION; ESCHERICHIA COLI; HOMEOSTASIS; HUMANS; PROTEIN SUBUNITS; PROTEIN-SERINE-THREONINE KINASES; PYRONES; RECOMBINANT PROTEINS; THIOPHENES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77955420075     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.04.022     Document Type: Article

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