Efficient isolation and purification of five products from microbial biotransformation of cinobufagin by high-speed counter-current chromatography

Journal of Separation Science

Volumn 33, Issue 15, 2010, Pages 2272-2277

  Ma, Xiao Chi ^a   Xin, Xiulan ^b   Zhang, Bao Jing ^a   Deng, Sha ^a   Yao, Ji Hong ^a   Wang, Chang Yuan ^a   Cui, Jian ^b   Tian, Yan ^a   Liu, Ke Xin ^a  

^a DALIAN MEDICAL UNIVERSITY   (China)

^b Beijing Vocational College of Electronic Science and Technology   (China)

Author keywords

Bufo gararizans; Cytotoxicity; High speed counter current chromatography; Microbial biotransforamtion; Preparative chromatography

Indexed keywords

CHEMICAL STRUCTURE; CORDYCEPS MILITARIS; CRUDE EXTRACT; CYTOTOXIC; ETHYL ACETATES; HIGH-SPEED COUNTER CURRENT CHROMATOGRAPHY; IN-VITRO; ISOLATION AND PURIFICATION; MALIGNANT MELANOMA; METHANOL-WATER; N-HEXANE; POTENT ACTIVITY; PREPARATIVE CHROMATOGRAPHY; TWO-PHASE SOLVENT SYSTEM;

ESTERS; HEXANE; METHANOL; ORGANIC SOLVENTS; SPEED;

CHROMATOGRAPHY;

12BETA HYDROXYL CINOBUFAGIN; 3 KETO CINOBUFAGIN; 4BETA,12ALPHA DIHYDROXYL CINOBUFAGIN; 5BETA HYDROXYL CINOBUFAGIN; ANTINEOPLASTIC AGENT; CINOBUFAGIN; DEACETYLCINOBUFAGIN; UNCLASSIFIED DRUG;

ARTICLE; BIOTRANSFORMATION; CANCER CELL CULTURE; CARBON NUCLEAR MAGNETIC RESONANCE; CHROMATOGRAPHY; CORDYCEPS; DRUG ISOLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH SPEED COUNTER CURRENT CHROMATOGRAPHY; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; PURIFICATION;

ANTINEOPLASTIC AGENTS; BIOTRANSFORMATION; BUFANOLIDES; CELL LINE, TUMOR; CELL PROLIFERATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CORDYCEPS; COUNTERCURRENT DISTRIBUTION; DRUG SCREENING ASSAYS, ANTITUMOR; HELA CELLS; HUMANS; MOLECULAR CONFORMATION; PLANT EXTRACTS; STEREOISOMERISM;

BUFO; CORDYCEPS MILITARIS;

EID: 77955367196     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.201000212     Document Type: Article

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