Purification and properties of a lipase from thermophilic Geobadllus stearothermophilus strain-5

International Journal of Biological Chemistry

Volumn 4, Issue 4, 2010, Pages 203-212

  Sifour, M ^a,b   Saeed, H M ^b   Zaghloul, T I ^b   Berekaa, M M ^c   Abdel Fattah, Y R ^d  

^a UNIVERSITY OF JIJEL   (Algeria)

^b ALEXANDRIA UNIVERSITY   (Egypt)

^c Alexandria University Faculty Of Science   (Egypt)

^d GENETIC ENGINEERING AND BIOTECHNOLOGY RESEARCH INSTITUTE   (Egypt)

Author keywords

Characterization; Enzyme stability; Geobacillus stearothermophilus; Purification; Thermostable lipase

Indexed keywords

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 77955102995     PISSN: 1819155X     EISSN: None     Source Type: Journal    
DOI: 10.3923/ijbc.2010.203.212     Document Type: Article

References (29)

1. Abdel-Fattah, Y.R. and A.A. Gaballa, 2008. Identification and over-expression of a thermostable lipase from Geobacillus thermoleovorans Toshki in Escherichia coli. Microbiol. Res., 163: 13-20.
2. Berekaa, M., T.I. Zaghloul, Y.R. Abdel-Fattah, H.M. Saeed and M. Sifour, 2009. Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5. World J. Microbiol. Biotechnol., 25: 287-294.
3. Castro-Ochoa, L.D., C. Rodriguez-Gomez, G. Valerio-Alfaro and R.O. Ros, 2005. Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme Microb. Technol., 37: 648-654.
4. Colak, A., D. Sisik, N. Saglam, S. Guner, S. Canakci and A.O. Belduz, 2005. Characterization of a thermoalkalophilic esterase from a novel thermophilic bacterium, Anoxybacillus gonensis G2. Bioresour. Technol., 96: 625-631.
5. Dharmsthiti, S. and S. Luchai, 1999. Production, purification and characterization of thermophilic lipase horn Bacillus sp. THL027. FEMS Microbiol. Lett., 179: 241-246.
6. Ewis, H.E., A.T. Abdelal and CD. Lu, 2004. Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus. Gene, 329: 187-195.
7. Fucinos, P., CM. Abadin, A. Sanroman, M.A. Longo, L. Pastrana and M.L. Rua, 2005. Identification of extracellular lipases/esterases produced by Thermus thermophilus HB27: Partial purification and preliminary biochemical characterization. J. Biotechnol., 117: 233-241.
8. Gupta, R., N. Gupta and P. Rathi, 2004. Bacterial lipases: An overview of production, purification and biochemical properties. Applied Microbiol. Biotechnol., 64: 763-781.
9. Haki, G.D. and S.K. Rakshit, 2003. Developments in industrially important thermostable enzymes: A review. Bioresource Technol., 89: 17-34.
10. Hasan, F., A.A. Shah and A. Hameed, 2006. Industrial applications of microbial lipases. Enzyme Microb. Technol., 39: 235-251.
11. Kambourova, M., N. Kirilova, R. Mandeva and A. Derekova, 2003. Purification and properties of thermostable lipase from a thermophilic Bacillus stearothermophilus MC 7. J. Mol. Cat. B: Enz.,22: 307-313.
12. Kermasha, S., M. Safari and B. Bisakowski, 1998. Characterization of purified lipase fraction from Rhizopus niveus. J. Agric. Food Chem., 46: 4451-4456.
13. Kim, M.H., H.K. Kim, J.K. Lee, S. Y. Park and T.K. Oh, 2000. Thermostable lipase of Bacillus stearothermophilus: High-level production, purification and calcium-dependent thermostabiliry. Biosci. Biotechnol. Biochem., 64: 280-286.
14. 4. Nature, 227: 680-685.
15. Lee, D.W., Y.S. Koh, K. Kim, B. Kim and H. Choi et al., 1999. Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1. FEMS Microbiol. Lett., 179: 393-400.
16. Lee, D.W., H.W. Kim, K.W. Lee, B.C. Kim and E.A. Choe et al, 2001. Purification and characterization of two distinct thermostable lipases from the gram-positive thermophilic bacterium Bacillus thermoleovorans ID-1. Enzyme Microb. Technol., 29: 363-371.
17. Li, H. and X. Zhang, 2005. Characterization of thermostable lipase thermophilic Geobacillus sp. TW1. Protein Expression Purif, 42: 153-159.
18. Lowry, O.H.,N.J. Rosebrough, A.L. Farr andR.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
19. Nawani, N. and J. Kaur, 2000. Purification, characterization and thermostability of lipase from a thermophilic Bacillus sp. J33. Mol. Cell. Biochem., 206: 91 -96.
20. Rahman, R.N.Z., T.C. Leow, A.B. Salleh and M. Basn, 2007. Geobacillus zalihae sp. nov. strain TIT, a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia. BMC Microbiol, 7: 77-87.
21. Saeed, H.M., N.A. Soliman and Y.R. Abdel-Fattah, 2005. Production, purification and characterization of thermostable lipase enzyme from a thermophilic Bacillus sp. J. Med. Res. inst., 26: 7-11.
22. Schmidt-Dannert, C, H. Sztajer, W. Stocklein, U. Menge and R.D. Schmid, 1994. Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus. Biochim. Biophys. Acta., 1214: 43-53.
23. Schmidt-Dannert, C, M.L. Rua, H. Atomi and R.D. Schmid 1996. Thermoalkalophilic lipase of Bacillus thermocatenulatus. I. Molecular cloning, nucleotide sequence, purification and some properties. Biochim. Biophys. Acta., 1301: 105-114.
24. Sharma, R., S.K. Som, R.M. Vohra, L.K. Gupta and J.K. Gupta 2002. Purification and characterisation of a thermostable alkaline lipase from a new thermophilic Bacillus sp. RSJ-1. Process Biochem., 37: 1075-1084.
25. Sinchaikul, S., B. Sookkheo, S. Phutrakul, F. Pan and S.T. Chen, 2001. Optimization of a thermostable lipase from BacillusstearothermophilusVl: Overexpression, purification and characterization. Prot Express. Purif., 22: 388-398.
26. Soliman, N.A., M. Knoll, Y.R. Abdel-Fattah, R.D. Schmid and S. Lange, 2007. Molecular cloning and characterization of thermostable esterase and lipase from Geobacillus thermoleovorans YN isolated from desert soil in Egypt. Process Biochem., 42: 1090-1100.
27. Tyndall, J.D., S. Sinchaikul, L.A. Fothergill-Gilmore, P. Taylor and M.D. Walkinshaw, 2002. Crystal structure of a thermostable lipase from Bacillus stearothermophilus PI. J. Mol. Biol., 323: 859-869.
28. Vorderwuelbecke, T., K. Kieslich and H. Erdmann, 1992. Comparison of lipases by different assays. Enz. Microb. Technol., 14: 631-639.
29. Wang, Y., K.C. Srivastava, G.J. Shenand HJ. Wang, 1995. Thermostable alkaline lipase from a newly isolated thermophilic Bacillus strain A30-1 (ATCC 53841). J. Ferment Bioeng., 79: 433-438. 212