Thermostable Lipase of Bacillus stearothermophilus: High-level Production, Purification, and Calcium-dependent Thermostability

Bioscience, Biotechnology and Biochemistry

Volumn 64, Issue 2, 2000, Pages 280-286

  Kim, Myung Hee ^a   Kim, Hyung Kwoun ^a   Lee, Jung Kee ^a   Park, Sun Yang ^a   Oh, Tae Kwang ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Bacillus stearothermophilus; Calcium dependent thermostability; Lipase; Tryptophan fluorescence emission

Indexed keywords

CALCIUM; PRIMER DNA; TRIACYLGLYCEROL LIPASE;

ARTICLE; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; GEOBACILLUS STEAROTHERMOPHILUS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN FOLDING; TEMPERATURE;

BACILLUS STEAROTHERMOPHILUS; BASE SEQUENCE; CALCIUM; CHROMATOGRAPHY, ION EXCHANGE; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ESCHERICHIA COLI; KINETICS; LIPASE; PROTEIN FOLDING; TEMPERATURE;

EID: 0034134257     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.64.280     Document Type: Article

References (21)

1. Dor dick, J. S., Enzymatic catalysis in monophasic organic solvents. Enz. Microb. Technol., 11, 194-211 (1989).
2. Jaeger, K. E., Ransac, S., Dijkstra, B. W., Colson, C., van Heuvel, M., and Misset, O., Bacterial lipases. FEMS Microbiol. Rev., 15, 29-63 (1994).
3. Becker, P., Abu-Reesh, I., Markossian, S., An-tranikian, G., and Markl, EE, Determination of the kinetic parameters during continuous cultivation of the lipase-producing thermophile Bacillus sp. IHI-91 on olive oil. Appl. Microbiol. Biotechnol., 48, 184-190 (1997).
4. Schmidt-Dannert, C., Rua, M. L., Atomi, H., and Schmid, R. D., Thermoalkalophilic lipase of Bacillus thermocatenulatus. I. Molecular cloning, nucleotide sequence, purification and some properties. Biochim. Biophys. Acta., 1301, 105-114 (1996).
5. Sugihara, A., Ueshima, M., Shimada, Y., Tsunasawa, S., and Tominaga, Y., Purification and characterization of a novel thermostable lipase from Pseudomonas cepacia. J. Biochem., 112, 598-603 (1992).
6. Sugihara, A., Tani, T., and Tominaga, Y., Purification and characterization of a novel thermostable lipase from Bacillus sp. J. Biochem., 109, 211-216 (1991).
7. Chung, G. H., Lee, Y. P., Yoo, O. J., and Rhee, J. S., Overexpression of a thermostable lipase gene from Pseudomonas fluorescens in Escherichia coli. Appl. Microbiol. Biotechnol., 35, 237-241 (1991).
8. Kim, H. K., Park, S. Y., and Oh, T. K., Purification and partial characterization of thermostable carboxyl esterase from Bacillus stear othermophilus LI. J. Microbiol. Biotechnol., 7, 37-42 (1997).
9. Kim, H. K., Park, S. Y., Lee, J. K., and Oh, T. K., Gene cloning and characterization of thermostable lipase from Bacillus st ear other mophilus LI. Biosci. Biotechnol. Biochem., 62, 66-71 (1998).
10. Benzonana, G., On the role of calcium ions during the hydrolysis of insoluble triglycerides by pancreatic lipase in the presence of bile salts. Biochim. Biophys. Acta., 151, 137-146 (1968).
11. Benzonana, G., Action of some effectors on the hydrolysis of long-chain triglycerides by pancreatic lipase. Biochim. Biophys. Acta., 164, 47-58 (1968).
12. Gubenator, K., Muller, K., and Winkler, F. K., The structure of human pancreatic lipase suggests a locally inverted, trypsin-like mechanism. In “Lipase: structure, mechanism and genetic engineering”, eds. Alberghina, L., Schmid, R. D., and Verger, R., VCH, Weingeim/New York, pp. 9-16 (1990).
13. Noble, M. E. M., Cleasby, A., Johnson, L. N., Eg-mond, M. R., and Frenken, L. G. J., The crystal structure of triacylglycerol lipase from Pseudomonas glumae reveals a partially redundant catalytic aspartate. FEBS Lett., 331, 123-128 (1993).
14. San Clemente, C. L. and Vadegra, D. V., Instrumental assay of microbial lipase at constant pH. Appl. Environ. Microbiol., 15, 110-113 (1967).
15. Rua, M. L., Atomi, H., Schmidt-Dannert, C., and Schmid, R. D., High-level expression of the ther-moalkalophilic lipase from Bacillus thermocatenula-tus in Escherichia coli. Appl. Microbiol. Biotechnol., 49, 405-410 (1998).
16. Taipa, M. A., Aires-Barros, M. R., and Cabral, J. M. S., Purification of lipases. J. Biotechnol., 26, 111-142 (1992).
17. Jaeger, K. E., Adrian, F. J., Meyer, H. E., Hancock, R. E. W., and Winkler, U. K., Extracellular lipase from Pseudomonas aeruginosa is an amphiphilic protein. Biochim. Biophys. Acta., 1120, 315-321 (1992).
18. Diinhaupt, A., Lang, S., and Wagner, F., Pseudomonas cepacia lipase: studies on aggregation, purification and on the cleavage of olive oil. Biotechnol. Lett., 14, 953-958 (1992).
19. Rua, M. L., Schmidt-Dannert, C., Wahl, S., Sprauer, A., and Schmid, R. D., Thermoalkalophilic lipase of Bacillus thermocatenulatus: large-scale production, purification and properties: aggregation behavior and its effect on activity. J. Biotechnol., 56, 89-102 (1997).
20. Choo, D. W., Kurihara, T., Suzuki, T., Soda, K., and Esaki, N., A cold-adapted lipase of an Alaskan psychrotroph, Pseudomonas sp. strain Bll-1: gene cloning and enzyme purification and characterization. Appl. Environ. Microbiol., 64, 486-491 (1998).
21. Simons, J. W., van Kampen, M. D., Ubarretxena-Belandia, I., Cox, R. C., Alves dos Santos, C. M., Egmond, M. R., and Verheij, H. M., Identification of a calcium binding site in Staphylococcus hyicus lipase: generation of calcium-independent variants. Biochemistry, 38, 2-10 (1999).