Brønsted analysis of an enzyme-catalyzed pseudo-deglycosylation reaction: Mechanism of desialylation in sialidases

Biochemistry

Volumn 49, Issue 30, 2010, Pages 6473-6484

  Shidmoossavee, Fahimeh S ^a   Cheng, Lydia ^a   Watson, Jacqueline N ^a   Bennet, Andrew J ^a  

^a SIMON FRASER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARYLOXIDE; BINDING SUBSTRATES; C-O CLEAVAGE; CONJUGATE ACID; DEGLYCOSYLATION; DESIALYLATION; DEUTERIUM KINETIC ISOTOPE EFFECT; DIFLUOROPHENYL; HYDROLYSIS REACTION; KINETIC DATA; LEAVING GROUPS; RATE LIMITING; REACTION STEPS; SIALIDASES; SIALOSIDE; TRANSITION STATE; WILD TYPES;

DEUTERIUM; ENZYMES; HYDROLYSIS; PHENOLS; SUBSTRATES; SUGARS;

REACTION INTERMEDIATES;

DEUTERIUM; METHYL[2 FLUOROPHENYL(5 ACETAMIDO 4,7,8,9 TETRA O ACETYL 3,5 DIDEOXY DEXTRO GLYCERO BETA DEXTRO GALACTO NON 2 ULOPYRANOSYL)]ONATE; METHYL[2,3 DIFLUOROPHENYL(5 ACETAMIDO 3,5 DIDEOXY DEXTRO GLYCERO BETA DEXTRO GALACTO NON 2 ULOPYRANOSYL)]ONATE; METHYL[2,5 DIFLUOROPHENYL(5 ACETAMIDO 4,7,8,9 TETRA O ACETYL 3,5 DIDEOXY DEXTRO GLYCERO BETA DEXTRO GALACTO NON 2 ULOPYRANOSYL)]ONATE; METHYL[2,6 DIFLUOROPHENYL(5 ACETAMIDO 3,5 DIDEOXY DEXTRO GLYCERO BETA DEXTRO GALACTO NON 2 ULOPYRANOSYL)]ONATE; METHYL[3 FLUOROPHENYL(5 ACETAMIDO 4,7,8,9 TETRA O ACETYL 3,5 DIDEOXY DEXTRO GLYCERO BETA DEXTRO GALACTO NON 2 ULOPYRANOSYL)]ONATE; METHYL[3,5 DIFLUOROPHENYL(5 ACETAMIDO 4,7,8,9 TETRA O ACETYL 3,5 DIDEOXY DEXTRO GLYCERO BETA DEXTRO GALACTO NON 2 ULOPYRANOSYL)]ONATE; METHYL[4 FLUOROPHENYL(5 ACETAMIDO 4,7,8,9 TETRA O ACETYL 3,5 DIDEOXY DEXTRO GLYCERO BETA DEXTRO GALACTO NON 2 ULOPYRANOSYL)]ONATE; SIALIDASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; DEGLYCOSYLATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME MECHANISM; ENZYME SYNTHESIS; HYDROLYSIS; IC 50; MICROMONOSPORA; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; WILD TYPE;

CATALYSIS; GLYCOSYLATION; HYDROLYSIS; KINETICS; MICROMONOSPORA; MUTATION, MISSENSE; N-ACETYLNEURAMINIC ACID; NEURAMINIDASE; SUBSTRATE SPECIFICITY;

MICROMONOSPORA VIRIDIFACIENS;

EID: 77955035317     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi100513u     Document Type: Article

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