The hydrolase and transferase activity of an inverting mutant sialidase using non-natural β-sialoside substrates

Biochemistry

Volumn 45, Issue 44, 2006, Pages 13264-13275

  Watson, Jacqueline N ^a   Indurugalla, Deepani ^a   Cheng, Lydia L ^a   Narine, Arun A ^a   Bennet, Andrew J ^a  

^a SIMON FRASER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYME KINETICS; MUTAGENESIS; RATE CONSTANTS; SUBSTRATES;

HYDROLASE; MUTANTS; SIALIDASE; TRANSFERASE;

ENZYMES;

4 METHYLUMBELLIFERYL BETA SIALOSIDE; 4 NITROPHENYL 2 THIO BETA SIALOSIDE; BETA SIALOSIDE; HYDROLASE; LACTOSE; MUTANT PROTEIN; PHENYL GROUP; SIALIC ACID DERIVATIVE; SIALIDASE; TRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; COLUMN CHROMATOGRAPHY; DNA SEQUENCE; ENZYME KINETICS; ENZYME SUBSTRATE; ENZYMOLOGY; GLYCOSYLATION; HYDROLYSIS; MICHAELIS MENTEN KINETICS; PH; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; HYDROGEN PEROXIDE; HYDROLASES; KINETICS; MICROMONOSPORA; MODELS, MOLECULAR; NEURAMINIDASE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OXYGEN; SIALIC ACIDS; SUBSTRATE SPECIFICITY; TRANSFERASES;

MICROMONOSPORA VIRIDIFACIENS;

EID: 33750737735     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi061489x     Document Type: Article

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