Gram-specific quantitative polymerase chain reaction for diagnosis of neonatal sepsis: Implications for clinical practice

Critical Care Medicine

Volumn 37, Issue 8, 2009, Pages 2487-2488

  Frayha, Husn H ^a   Kalloghlian, Avedis ^a  

^a KING FAISAL SPECIALIST HOSPITAL AND RESEARCH CENTER   (Saudi Arabia)

Author keywords

Bacterial infections; Gram specific quantitative polymerase chain reaction; Neonatal sepsis; Polymerase chain reaction; Quantitative polymerase chain reaction

Indexed keywords

DNA 16S; RNA 16S;

BACTERIUM IDENTIFICATION; BLOOD CULTURE; CLINICAL PRACTICE; DIAGNOSTIC ACCURACY; EDITORIAL; GRAM NEGATIVE INFECTION; GRAM POSITIVE INFECTION; HUMAN; NEWBORN SEPSIS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA GENE; SENSITIVITY AND SPECIFICITY;

EID: 68249101552     PISSN: 00903493     EISSN: 15300293     Source Type: Journal    
DOI: 10.1097/CCM.0b013e3181af1bd1     Document Type: Editorial

References (15)

1. McIntire DD, Bloom SL, Casey BM, et al: Birth weight in relation to morbidity and mortality among newborn infants. N Engl J Med 1999; 340:1234-1238
2. Kaufman D, Fairchild KD: Clinical microbiology of bacterial and fungal sepsis in very-low-birth-weight infants. Clin Microbiol Rev 2004; 17:638-680
3. Neal PR, Kleiman MB, Reynolds JK, et al: Volume of blood submitted for culture from neonates. J Clin Microbiol 1986; 24:353-356
4. Laforgia N, Coppola B, Carbone R, et al: Rapid detection of neonatal sepsis using polymerase chain reaction. Acta Paediatr 1997; 86:1097-1099
5. Jordan JA, Durso MB: Comparison of 16S rRNA gene PCR and BACTEC 9240 for detection of neonatal bacteremia. J Clin Microbiol 2000; 38:2574-2578
6. Yadav AK, Wilson CG, Prasad PL, et al: Polymerase chain reaction in rapid diagnosis of neonatal sepsis. Indian Pediatr 2005; 42: 681-685
7. Shang S, Chen G, Wu Y, et al: Rapid diagnosis of bacterial sepsis with PCR amplification and microarray hybridization in 16S rRNA gene. Pediatr Res 2005; 58:143-148
8. Jordan JA, Durso MB, Butchko AR, et al: Evaluating the near-term infant for early onset sepsis: Progress and challenges to consider with 16S rDNA polymerase chain reaction testing. J Mol Diagn 2006; 8:357-363
9. Wu YD, Chen LH, Wu XJ, et al: Gram stain-specific-probe-based real-time PCR for diagnosis and discrimination of bacterial neonatal sepsis. J Clin Microbiol 2008; 46:2613-2619
10. Reir-Nilsen T, Farstad T, Nakstad B, et al: Comparison of broad range 16S rDNA PCR and conventional blood culture for diagnosis of sepsis in the newborn: A case control study. BMC Pediatr 2009; 9:5
11. Jordan JA, Butchko AR, Durso MB: Use of pryrosequencing of 16S rRNA fragments to differentiate between bacteria responsible for neonatal sepsis. J Mol Diagn 2005; 7:105-110
12. Jordan JA, Durso MB: Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis. J Mol Diagn 2005; 7:575-581
13. Andrade SS, Bispo PJM, Gales AC: Advances in the microbiological diagnosis of sepsis. Shock 2008; 30(Suppl 1):41-46
14. Chan KYY, Lam HS, Cheung HM, et al: Rapid identification and differentiation of Gramnegative and Gram-positive bacterial bloodstream infections by quantitative polymerase chain reaction in preterm infants. Crit Care Med 2009; 37:2441-2447
15. Mussap M, Molinari MP, Senno E, et al: New diagnostic tools for neonatal sepsis: The role of a real-time polymerase chain reaction for the early detection and identification of bacterial and fungal species in blood samples. J Chemother 2007; 19(Suppl 2):31-34