Real-time PCR of the 16S-rRNA gene in the diagnosis of neonatal bacteraemia

Acta Paediatrica, International Journal of Paediatrics

Volumn 97, Issue 10, 2008, Pages 1376-1380

  Ohlin, Andreas ^a   Bäckman, Anders ^a   Björkqvist, Maria ^a   Mölling, Paula ^a   Jurstrand, Margaretha ^a   Schollin, Jens ^a  

^a ÖREBRO UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Diagnostic test; Neonatal sepsis; Polymerase chain reaction

Indexed keywords

RNA 16S;

ARTICLE; BACTEREMIA; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BLOOD CULTURE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONFIDENCE INTERVAL; CONTROLLED STUDY; DNA SEQUENCE; FEMALE; GRAM NEGATIVE SEPSIS; HUMAN; INFANT; MAJOR CLINICAL STUDY; MALE; NEWBORN; NEWBORN SEPSIS; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

BACTEREMIA; BASE SEQUENCE; DIAGNOSTIC TESTS, ROUTINE; HUMANS; INFANT, NEWBORN; PILOT PROJECTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RISK ASSESSMENT; RNA PROBES; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 51349126199     PISSN: 08035253     EISSN: 16512227     Source Type: Journal    
DOI: 10.1111/j.1651-2227.2008.00924.x     Document Type: Article

References (13)

1. Malik A, Hui CP, Pennie RA, Kirpalani H. Beyond the complete blood cell count and C-reactive protein: a systematic review of modern diagnostic tests for neonatal sepsis. Arch Pediatr Adolesc Med 2003 157 : 511 6.
2. Bizzarro MJ, Raskind C, Baltimore RS, Gallagher PG. Seventy-five years of neonatal sepsis at Yale: 1928-2003. Pediatrics 2005 116 : 595 602.
3. Fenollar F, Raoult D. Molecular genetic methods for the diagnosis of fastidious microorganisms. Apmis 2004 112 : 785 807.
4. Laforgia N, Coppola B, Carbone R, Grassi A, Mautone A, Iolascon A. Rapid detection of neonatal sepsis using polymerase chain reaction. Acta Paediatr 1997 86 : 1097 9.
5. Jordan JA, Durso MB. Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis. J Mol Diagn 2005 7 : 575 81.
6. Jordan JA, Butchko AR, Durso MB. Use of pyrosequencing of 16S rRNA fragments to differentiate between bacteria responsible for neonatal sepsis. J Mol Diagn 2005 7 : 105 10.
7. Greisen K, Loeffelholz M, Purohit A, Leong D. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J Clin Microbiol 1994 32 : 335 51.
8. Gharizadeh B, Ohlin A, Mölling P, Bäckman A, Amini B, Olcén P, et al. Multiple group-specific sequencing primers for reliable and rapid DNA sequencing. Mol Cell Probes 2003 17 : 203 10.
9. Jaffe RI, Lane JD, Albury SV, Niemeyer DM. Rapid extraction from and direct identification in clinical samples of methicillin-resistant staphylococci using the PCR. J Clin Microbiol 2000 38 : 3407 12.
10. Jordan JA, Durso MB. Comparison of 16S rRNA gene PCR and BACTEC 9240 for detection of neonatal bacteremia. J Clin Microbiol 2000 38 : 2574 8.
11. Tapp J, Thollesson M, Herrmann B. Phylogenetic relationships and genotyping of the genus Streptococcus by sequence determination of the RNase P RNA gene, rnpB. Int J Syst Evol Microbiol 2003 53 (Pt 6 1861 71.
12. Harris KA, Hartley JC. Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service. J Med Microbiol 2003 52 : 685 91.
13. Shang S, Chen G, Wu Y, Du L, Zhao Z. Rapid diagnosis of bacterial sepsis with PCR amplification and microarray hybridization in 16S rRNA gene. Pediatr Res 2005 58 : 143 8.