Diagnostic performance of different fecal Lawsonia intracellularis-specific polymerase chain reaction assays as diagnostic tests for proliferative enteropathy in pigs: A review

Journal of Veterinary Diagnostic Investigation

Volumn 22, Issue 4, 2010, Pages 487-494

  Pedersen, Ken Steen ^a   Holyoake, Patricia ^b   Stege, Helle ^a   Nielsen, Jens Peter ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b NSW DEPARTMENT OF PRIMARY INDUSTRIES   (Australia)

Author keywords

Lawsonia intracellularis; Pigs; Polymerase chain reaction; Proliferative enteropathy; Sensitivity; Specificity

Indexed keywords

LAWSONIA INTRACELLULARIS; SUIDAE;

EID: 77954923294     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063871002200401     Document Type: Review

References (48)

1. Baccaro MR, Moreno AM, Shinya LT, Dotto DS: 2003, Identification of bacterial agents of enteric diseases by multiplex PCR in growing-finishing pigs. Braz J Microbiol 34:225-229.
2. Biester HE, Schwarte LH: 1931, Intestinal adenoma in swine. Am J Pathol 7:175-185.
3. Biksi I, Kacskovics I, Mandoki M, Ivan J: 1998, Detection of Lawsonia intracellularis in Hungarian swine herds by polymerase chain reaction. Acta Vet Hung 46:415-420.
4. Boesen HT, Jensen TK, Schmidt AS, et al.: 2004, The influence of diet on Lawsonia intracellularis colonization in pigs upon experimental challenge. Vet Microbiol 103:35-45.
5. Chang WL, Wu CF, Wu Y, et al.: 1997, Prevalence of Lawsonia intracellularis in swine herds in Taiwan. Vet Rec 26:103-104.
6. Collins A, Love R: 2007, Re-challenge of pigs following recovery from proliferative enteropathy. Vet Microbiol 120:381-386.
7. Collins A, Love RJ, Pozo J: 2000, Studies on the ex vivo survival of Lawsonia intracellularis. J Swine Health Prod 8:211-215.
8. Dias CA, Gonzalez AM, Escatel GS, et al.: 1998, Diagnósticode ilei{dotless}́tis porcina por medio de la reacción en cadena de la polimerasa [Polymerase chain reaction for diagnosis of porcine ileitis]. Vet Mex 29:263-267. In Spanish.
9. Dü nser M, Schweighardt H, Krassnig G: 1997, Nachweis von Lawsonia intracellularis, des Erregers der Proliferativen Enteropathien des Schweines (Adenomatose-Komplex), mittels Polymerase-Kettenreaction (PCR) in Oberö sterreich [Detection in Austria of Lawsonia intracellularis, the causal agent of proliferative enteropathy in pigs, by the polymerase chain reaction]. Wien Tierarztl Monatsschr 84:307-313. In German.
10. Elder RO, Duhamel GE, Mathiesen MR, et al.: 1997, Multiplex polymerase chain reaction for simultaneous detection of Lawsonia intracellularis, Serpulina hyodysenteriae, and salmonellae in porcine intestinal specimens. J Vet Diagn Invest 9:281-286.
11. Gebhart CJ, McOrist S, Lawson GH, et al.: 1994, Specific in situ hybridization of the intracellular organism of porcine proliferative enteropathy. Vet Pathol 31:462-467.
12. Greiner M, Gardner IA: 2000, Application of diagnostic tests in veterinary epidemiologic studies. Prev Vet Med 45:43-59.
13. Greiner M, Gardner IA: 2000, Epidemiologic issues in the validation of veterinary diagnostic tests. Prev Vet Med 45:3-22.
14. Guedes RMC, Gebhart CJ: 2003, Comparison of intestinal mucosa homogenate and pure culture of the homologous Lawsonia intracellularis isolate in reproducing proliferative enteropathy in swine. Vet Microbiol 93:159-166.
15. Guedes RMC, Gebhart CJ: 2003, Onset and duration of fecal shedding, cell-mediated and humoral immune responses in pigs after challenge with a pathogenic isolate or attenuated vaccine strain of Lawsonia intracellularis. Vet Microbiol 91:135-145.
16. Guedes RMC, Gebhart CJ, Winkelman NL, et al.: 2002, Comparison of different methods for diagnosis of porcine proliferative enteropathy. Can J Vet Res 66:99-107.
17. Jacobson M, Aspan A, Heldtander Konigsson M, et al.: 2004, Routine diagnostics of Lawsonia intracellularis performed by PCR, serologic and post mortem examination, with special emphasis on sample preparation methods for PCR. Vet Microbiol 102:189-201.
18. Jones G, Ward G, Murtaugh M, et al.: 1993, Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction. J Clin Microbiol 31:2611-2615.
19. Jones G, Ward G, Murtaugh M, et al.: 1993, Relationship between ileal symbiont intracellularis and porcine proliferative enteritis. Infect Immun 61:5237-5244.
20. Jordan DM, Knitted JP, Schwartz KJ, et al.: 2004, A Lawsonia intracellularis transmission study using a pure culture inoculated seeder-pig sentinel model. Vet Microbiol 104:83-90.
21. Knittel JP, Jordan DM, Schwartz KJ, et al.: 1998, Evaluation of antemortem polymerase chain reaction and serologic methods for detection of Lawsonia intracellularis-exposed pigs. Am J Vet Res 59:722-726.
22. Kroll J, Roof M, Hoffman L, et al.: 2005, Proliferative enteropathy: a global enteric disease of pigs caused by Lawsonia intracellularis. Anim Health Res Rev 6:173-197.
23. Kroll J, Roof M, McOrist S: 2004, Evaluation of protective immunity in pigs following oral administration of an avirulent live vaccine of Lawsonia intracellularis. Am J Vet Res 65:559-565.
24. La T, Collins AM, Phillips ND, et al.: 2006, Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine feces. Lett Appl Microbiol 42:284-288.
25. Ladinig A, Sommerfeld-Stur I, Weissenbö ck H: 2009, Comparative evaluation of diagnostic methods for Lawsonia intracellularis infection in pigs, with emphasis on cases lacking characteristic lesions. J Comp Pathol 140:140-148.
26. Lawson GHK, Gebhart CJ: 2000, Proliferative enteropathy. J Comp Pathol 122:77-100.
27. Lindecrona RH, Jensen TK, Andersen PH, Moller K: 2002, Application of a 59 nuclease assay for detection of Lawsonia intracellularis in fecal samples from pigs. J Clin Microbiol 40:984-987.
28. McCormick BM, Hasse D, Monckton P: 1995, Detection of ileal symbiont intracellularis in porcine fecal samples by polymerase chain reaction. Vet Microbiol 47:387-393.
29. McOrist S: 2005, Defining the full costs of endemic porcine proliferative enteropathy. Vet J 170:8-9.
30. McOrist S, Boid R, Lawson GHK, McConnell I: 1987, Monoclonal antibodies to intracellular campylobacter-like organisms of the porcine proliferative enteropathies. Vet Rec 121:421-422.
31. McOrist S, Gebhart CJ: 1999, Proliferative enteropathies. In: Diseases of swine, ed. Straw BE, Zimmerman JJ, D'Allaire S, TaylorDJ, 8th ed., pp. 727-738. Blackwell Publishing,Ames, IA.
32. McOrist S, Gebhart CJ, Boid R, Barns SM: 1995, Characterization of Lawsonia intracellularis gen. nov., sp. nov., the obligate intracellular bacterium of porcine proliferative enteropathy. Int J Syst Bacteriol 45:820-825.
33. McOrist S, Gebhart CJ, Lawson GHK: 1994, Polymerase chain reaction for diagnosis of porcine proliferative enteropathy. Vet Microbiol 41:205-212.
34. Moller K, Jensen TK, Jorsal SE, et al.: 1998, Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly betahaemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhea among growing pigs. Vet Microbiol 62:59-72.
35. Nathues H, Holthaus K, Grosse Beilage E: 2009, Quantification of Lawsonia intracellularis in porcine faeces by real-time PCR. J Appl Microbiol 107:2009-2016.
36. Nathues H, Oliveira CJB, Wurm M, et al.: 2007, Simultaneous detection of Brachyspira hyodysenteriea, Brachyspira pilosicoli and Lawsonia intracellularis in porcine feces and tissue samples by multiplex-PCR. J Vet Med A Physiol Pathol Clin Med 54:532-538.
37. Nielsen SS, Houe H, Ersboell AK, Toft N: 2004, Evaluating diagnostic tests. In: Introduction to veterinary epidemiology, ed. Houe H, Ersboell AK, Toft N, 1st ed., pp. 133-151. Biofolia, Denmark.
38. Pejsak Z, Zmudzki J, Stankevicius A: 2001, Detection of Lawsonia intracellularis by one tube nested-PCR. Medycyna Wet 57:723-726.
39. Rowland AC: 1978, Necrotic enteritis and regional ileitis in pigs at slaughter. Vet Rec 100:338-339.
40. Rowland AC, Lawson GH, Maxwell A: 1973, Intestinal adenomatosis in the pig: occurrence of a bacterium in affected cells. Nature 247:417.
41. Smith S, McOrist S: 1997, Development of persistent intestinal infection and excretion of Lawsonia intracellularis by piglets. Res Vet Sci 62:6-10.
42. Stege H, Jensen TK, Moller K, et al.: 2000, Prevalence of intestinal pathogens in Danish finishing pig herds. Prev Vet Med 46:279-292.
43. Stege H, Jensen T, Moller K, et al.: 2004, Infection dynamics of Lawsonia intracellularis in pig herds. Vet Microbiol 104:197-206.
44. Suh D, Lym S, Bae Y, et al.: 2000, Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR. J Vet Sci 1:33-37.
45. Walter D, Knittel J, Schwartz K, et al.: 2001, Treatment and control of porcine proliferative enteropathy using different tiamulin delivery methods. J Swine Health Prod 9: 109-115.
46. Ward GE, Winkelman NL: 1990, Recognizing the three forms of PE in swine. Vet Med 85:197-203.
47. Zhang P, Gebhart CJ, Burden D, Duhamel GE: 2000, Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay (PCR-ELOSA). Mol Cell Probes 14:101-108.
48. Zmudzki J, Osek J, Stankevicius A, Pejsak Z: 2004, Rapid detection of Brachyspira hyodysenteriae and Lawsonia intracellularis in swine fecal and mucosal specimens by multiplex PCR. Bull Vet Inst Pulawy 48:207-214.