Evaluation of antemortem polymerase chain reaction and serologic methods for detection of Lawsonia intracellularis-exposed pigs

American Journal of Veterinary Research

Volumn 59, Issue 6, 1998, Pages 722-726

  Knittel, Jeffrey P ^a   Jordan, Dianna M ^b   Schwartz, Kent J ^b   Janke, Bruce H ^b   Roof, Michael B ^a   McOrist, Steven ^c   Harris, D L ^b  

^a BOEHRINGER INGELHEIM VETMEDICA INC   (United States)

^b IOWA STATE UNIVERSITY   (United States)

^c Veterinary Pathology Services   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIUM ANTIBODY; IMMUNOGLOBULIN G;

ANIMAL; ANIMAL DISEASE; ARTICLE; BLOOD; COMPARATIVE STUDY; FECES; FLUORESCENT ANTIBODY TECHNIQUE; GRAM NEGATIVE BACTERIUM; GRAM NEGATIVE INFECTION; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SWINE; SWINE DISEASE;

ANIMALS; ANTIBODIES, BACTERIAL; DNA, BACTERIAL; FECES; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GRAM-NEGATIVE BACTERIA; GRAM-NEGATIVE BACTERIAL INFECTIONS; IMMUNOGLOBULIN G; POLYMERASE CHAIN REACTION; SWINE; SWINE DISEASES;

EID: 0032091137     PISSN: 00029645     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Rowland AC, Lawson GHK. Porcine proliferative enteropathies. In: Leman AD, Straw BE, Mengeling WL, et al, eds. Diseases of swine. 7th ed. Ames, Iowa: Iowa State University Press, 1992;560-569.
2. Rowland AC, Lawson GHK. Intestinal adenomatosis in the pig: immunofluorescent and electron microscopic studies. Res Vet Sci 1974;17:323-330.
3. McOrist S, Gebhart CJ, Boid R, et al. Characterization of Lawsonia intracellularis gen. nov., sp. nov., the obligately intracellular bacterium of porcine proliferative enteropathy. Int J Syst Bacteriol 1995;45:820-825.
4. Gebhart CJ, Barnes SM, McOrist S. Ileal Symbiont intracellularis, an obligate intracellular bacterium of porcine intestines, showing a relationship to Desulfovibrio species. Int J Syst Bacteriol 1993;43:533-538.
5. Lawson GHK, McOrist S, Jasni S, et al. Intracellular bacteria of porcine proliferative enteropathy: cultivation and maintenance in vitro. Clin Microbiol 1993;31:1136-1142.
6. McOrist S, Boid R, Lawson GHK, et al. Monoclonal antibodies to intracellular campylobacter-like organisms of the porcine proliferative enteropathies. Vet Rec 1987;121:421-422.
7. Jones GF, Ward GE, Murtaugh MP, et al. Enhanced detection of intracellular organism of swine proliferative enteritis Ileal Symbiont intracellularis in feces by polymerase chain reaction. J Clin Microbiol 1993;31:2611-2615.
8. Jones GF, Ward GE, Murtaugh MP. Relationship between Ileal Symbiont intracellularis and porcine proliferative enteritis. Infect Immun 1993;61:5237-5244.
9. Knittel JP, Schwartz KJ, McOrist S, et al. Diagnosis of porcine proliferative enteritis. Compend Contin Educ Pract Vet 1997;19: S26-S29.
10. Lawson GHK, McOrist S, Rowland AC, et al. Serological diagnosis of the porcine proliferative enteropathies: implications for aetiology and epidemiology. Vet Rec 1988;122:554-557.
11. Holyoake PK, Cutler RS, Caple IW, et al. Enzyme-linked immunosorbent assay for measuring Ileal Symbiont intracellularis-specific immunoglobulin G response in sera of pigs. J Clin Microbiol 1994;32:1980-1985.
12. Bovarnick TE, Miller JC, Snyder JC. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol 1950;59:509-522.
13. Reed LJ, Muench H. A simple method of estimating fifty percent endpoints. Am J Hyg 1936;27:493-497.
14. Holyoake PK, Jones GF, Davies PR, et al. Application of a polymerase chain reaction assay for detection of proliferative enteritis-affected swine herds. J Vet Diagn Invest 1996;8:181-185.
15. McOrist S, Gebhart CJ, Lawson GHK. Polymerase chain reaction for diagnosis of porcine proliferative enteropathy. Vet Microbiol 1994;41:201-212.