Quantification of Lawsonia intracellularis in porcine faeces by real-time PCR

Journal of Applied Microbiology

Volumn 107, Issue 6, 2009, Pages 2009-2016

  Nathues, H ^a   Holthaus, K ^a   Grosse Beilage, E ^a  

^a UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

Author keywords

Ileitis; Lawsonia intracellularis; Real time PCR; Swine; Validation

Indexed keywords

ELECTROPHORESIS;

DETECTION LIMITS; ILEITI; LAWSONIUM INTRACELLULARI; LINEARITY RANGE; PLASMID DNA; QUANTIFICATION LIMIT; QUANTIFICATION METHODS; REAL-TIME PCR; SWINE; VALIDATION;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA;

BACTERIUM; DETECTION METHOD; DNA; ELECTROKINESIS; FECES; GEL; PIG; PLASMID; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; REGRESSION ANALYSIS;

ARTICLE; BACTERIUM DETECTION; DESULFOVIBRIONACEAE INFECTION; DIAGNOSTIC ACCURACY; FECES ANALYSIS; ILEITIS; LAWSONIA; LAWSONIA INTRACELLULARIS; LOGISTIC REGRESSION ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SWINE; VALIDATION PROCESS;

LAWSONIA INTRACELLULARIS; SUIDAE; SUS;

EID: 72849133584     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04389.x     Document Type: Article

References (25)

1. Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389 3402.
2. Bustin, S.A. (2005) Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences. Expert Rev Mol Diagn 5, 493 498.
3. Bustin, S.A. Nolan, T. (2004) Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Tech 15, 155 166.
4. Dauvillier, J., Picandet, V., Harel, J., Gottschalk, M., Desrosiers, R., Jean, D. Lavoie, J.P. (2006) Diagnostic and epidemiological features of Lawsonia intracellularis enteropathy in 2 foals. Can Vet J 47, 689 691.
5. Deuter, R., Pietsch, S., Hertel, S. Muller, O. (1995) A method for preparation of fecal DNA suitable for PCR. Nucleic Acids Res 23, 3800 3801.
6. Guedes, R.M. Gebhart, C.J. (2003) Onset and duration of fecal shedding, cell-mediated and humoral immune responses in pigs after challenge with a pathogenic isolate or attenuated vaccine strain of Lawsonia intracellularis. Vet Microbiol 91, 135 145.
7. Guedes, R.M., Winkelman, N.L. Gebhart, C.J. (2003) Relationship between the severity of porcine proliferative enteropathy and the infectious dose of Lawsonia intracellularis. Vet Rec 153, 432 433.
8. Jacobson, M., Aspan, A., Konigsson, M.H., Segerstad, C.H., Wallgren, P., Fellstrom, C., Jensen-Waern, M. Gunnarson, A. (2004) Routine diagnostics of Lawsonia intracellularis performed by PCR, serological and post mortem examination, with special emphasis on sample preparation methods for PCR. Vet Microbiol 102, 189 201.
9. Jones, G.F., Ward, G.E., Murtaugh, M.P., Lin, G. Gebhart, C. (1993) Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction. J Clin Microbiol 31, 2611 2615.
10. Kroll, J.J., Roof, M.B. McOrist, S. (2004) Evaluation of protective immunity in pigs following oral administration of an avirulent live vaccine of Lawsonia intracellularis. Am J Vet Res 65, 559 565.
11. La, T., Phillips, N.D. Hampson, D.J. (2003) Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces. J Clin Microbiol 41, 3372 3375.
12. La, T., Collins, A.M., Phillips, N.D., Oksa, A. Hampson, D.J. (2006) Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces. Lett Appl Microbiol 42, 284 288.
13. Larionov, A., Krause, A. Miller, W. (2005) A standard curve based method for relative real time PCR data processing. BMC Bioinformatics 6, 62.
14. Li, M., Gong, J., Cottrill, M., Yu, H., de Lange, C., Burton, J. Topp, E. (2003a) Evaluation of QIAamp DNA Stool Mini Kit for ecological studies of gut microbiota. J Microbiol Meth 54, 13 20.
15. Lindecrona, R.H., Jensen, T.K., Andersen, P.H. Moller, K. (2002) Application of a 5′ nuclease assay for detection of Lawsonia intracellularis in fecal samples from pigs. J Clin Microbiol 40, 984 987.
16. McOrist, S. Gebhart, C. (1999) Porcine proliferative enteropathies. In Diseases of Swine ed. Straw, B., D'Allaire, S., Mengeling, W.L. Taylor, D.J. pp. 521 534. Ames : Iowa State University Press.
17. McOrist, S. Lawson, G.H. (1993) Porcine proliferative enteropathy. Vet Rec 132, 368.
18. McOrist, S., Boid, R., Lawson, G.H. McConnell, I. (1987) Monoclonal antibodies to intracellular campylobacter-like organisms of the porcine proliferative enteropathies. Vet Rec 121, 421 422.
19. Nathues, H. (2007) Literature survey of the diagnosis of porcine proliferative enteropathy. Berl Munch Tierarztl Wochenschr 120, 499 507.
20. Nathues, H. Grosse Beilage, E. (2008) Diagnosis of L. intracellularis infection in pigs after vaccination or antimicrobial treatment. Dtsch Tierarztl Wochenschr 115, 404 409.
21. Nathues, H., Oliveira, C.J., Wurm, M., Grosse Beilage, E. Givisiez, P.E. (2007) Simultaneous detection of Brachyspira hyodysenteriae, Brachyspira pilosicoli and Lawsonia intracellularis in porcine faeces and tissue samples by multiplex-PCR. J Vet Med A Physiol Pathol Clin Med 54, 532 538.
22. Pfaffl, M.W. (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29, e45.
23. Pfaffl, M.W., Tichopad, A., Prgomet, C. Neuvians, T.P. (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol Lett 26, 509 515.
24. Queipo-Ortuno, M.I., Tena, F., Colmenero, J.D. Morata, P. (2008) Comparison of seven commercial DNA extraction kits for the recovery of Brucella DNA from spiked human serum samples using real-time PCR. Eur J Clin Microbiol Infect Dis 27, 109 114.
25. Smith, S.H. McOrist, S. (1997) Development of persistent intestinal infection and excretion of Lawsonia intracellularis by piglets. Res Vet Sci 62, 6 10.