The anthocyanidin synthase gene from sweetpotato [Ipomoea batatas (L.) Lam]: Cloning, characterization and tissue expression analysis

African Journal of Biotechnology

Volumn 9, Issue 25, 2010, Pages 3748-3752

  Liu, Xiaoqiang ^a   Chen, Min ^a   Li, Mingyang ^a   Yang, Chunxian ^a   Fu, Yufan ^a   Zhang, Qitang ^a   Zeng, Lingjiang ^a   Liao, Zhihua ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

Anthocyanidin synthase; Characterization; Cloning; Expression profile; Purple fleshed; Sweet potato

Indexed keywords

ANTHOCYANIN; POLYPEPTIDE; PROANTHOCYANIDIN SYNTHASE; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; GENE EXPRESSION PROFILING; METABOLIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLANT LEAF; PLANT ROOT; PLANT STEM; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SWEET POTATO; TISSUE DISTRIBUTION;

IPOMOEA BATATAS;

EID: 77954879992     PISSN: 16845315     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Dixon RA, Steele CL (1999). Flavonoids and isoflavonoids: A gold mine for metabolic engineering. Trends Plant Sci. 4: 394-400.
2. Fu YF, Chen M, Ye XL, Zhang QT, Liao ZH, Yang CX (2007). Variation laws of anthocyanin content of roots and their relationships with major economic traits in purple-fleshed sweetpotato [Ipomoea Batatas (L.) Lam]. Sci. Agri. Sinic. 40(10): 2185-2192.
3. Gong Z, Yamazaki M, Sugiyama M, Tanaka Y, Saito K (1997). Cloning and molecular analysis of structural genes involved in anthocyanin biosynthesis and expressed in a forma-specific manner in Perilla frutescens. Plant Mol. Biol. 35: 915-927.
4. Harborne JB (1988). Introduction to Ecological Biochemistry, third ed. Academic Press, London.
5. Heid C, Stevens J, Livak K, Williams P (1996). Real time Quantitative PCR. Genome Methods: Genome Research. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
6. Holton TA, Cornish EC (1995). Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell, 7: 1071-1083.
7. Imbert MP, Seaforth CE, Williams DB (1966). The anthocyanin pigments of the sweetpotato (Ipomoea batatas [L.] Lam.). J. Am. Soc. Hortic. Sci. 88: 481-485.
8. Jin MK, Lian SC, Ngoh KG, Tet FC, Brouillard R (2003). Analysis and biological activities of anthocyanins. Phytochemistry, 64: 923-933
9. Kamei H, Hashimoto Y, Koide T, Kojima T, Hasegawa M (1998). Anti-tumor effect of methanol extracts from red and white wines. Canc. Biothera. Radiopharma, 13(6): 447-452.
10. Kim SH, Lee JR, Hong ST, Yoo YG, An G, Kim SR (2003). Molecular cloning and analysis of anthocyanin biosynthesis genes preferentially expressed in apple skin. Plant Sci. 165: 403-413.
11. Koes R, Quattrocchio R, Mol J (1994). The flavonoid biosynthetic pathway in plants: function and evolution. BioEssay, 16: 123-132.
12. Koes R, Verweij W, Quattrocchio F (2005). Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. Trends Plant Sci. 10: 236-242.
13. Lalusin AG, Ohta M, Fujimura T (2006). Temporal and spatial expression of genes involved in anthocyanin biosynthesis during sweet potato (/pomoea batatas [L.] Lam.) root development. Int. J. Plant Sci. 167(2): 249-256.
14. Luo HR, Santa Maria M, Benavides J, Zhang DP, Zhang YZ, Ghislain M (2006). Rapid genetic transformation of sweetpotato [/pomoea batatas (L.) Lam] via organogenesis. Afr. J. Biotechnol. 5(20): 1851-1857.
15. Mol J, Grotewold E, Koes R (1998). How genes paint flowers and seeds. Trends Plant Sci. 3: 212-217.
16. Rosati C, Cadic A, Duron M, Ingouff M, Simoneau P (1999). Molecular characterization of the anthocyanidin synthase gene in Forsythia intermedia reveals organ-specific expression during flower development. Plant Sci. 149: 73-79.
17. Springob K, Nakajima J, Yamazaki M, Saito K (2003). Recent advances in the biosynthesis and accumulation of anthocyanins. Nat. Prod. Rep. 20: 288-303.
18. Terahara N, Shimizu T, Kato Y, Nakamura M, Maitani T, Yamaguchi M, Goda Y (1999). Six diacylated anthocyanins from the storage roots of purple sweetpotato, Ipomoea batatas. Biosci. Biotechnol. Biochem. 63: 1420-1424.
19. Zhou W, Huang CT, Gong YF, Feng QL, Gao F (2010). Molecular Cloning and Expression Analysis of an ANS Gene Encoding Anthocyanidin Synthase from Purple-Fleshed Sweet Potato [Ipomoea batatas (L.) Lam]. Plant Mol. Biol. Rep. 28: 112-121
20. Zulin S, Bassa IA, Gabriml SL, Francis FJ (1992). Anthocyanin pigments of sweetpotatoes, Ipomoea batatas. J. Food Sci. 57: 755-757.