Optimization of cultivation conditions in spin tubes for Chinese hamster ovary cells producing erythropoietin and the comparison of glycosylation patterns in different cultivation vessels

Biotechnology Progress

Volumn 26, Issue 3, 2010, Pages 653-663

  Strnad, Jure ^a,b   Brinc, Matjaž ^a   Spudić, Vatroslav ^a   Jelnikar, Nadja ^a   Mirnik, Lidija ^a   Čarman, Barbara ^a   Kravanja, Zdravko ^b  

^a Lek Pharmaceuticals dd   (Slovenia)

^b UNIVERSITY OF MARIBOR   (Slovenia)

Author keywords

D optimal design; Design expert; Erythropoietin; Glycosylation; Spin tube

Indexed keywords

CELL LINES; CHINESE HAMSTER OVARY CELLS; CULTIVATION CONDITIONS; D-OPTIMAL; D-OPTIMAL DESIGN; D-OPTIMAL DESIGNS; DESIGN SPACES; ISOFORMS; PERFORMANCE PARAMETERS; PRODUCT QUALITY; SHAKE FLASKS; SHAKING RATE; SURFACE METHODS; WORKING VOLUME;

ANIMAL CELL CULTURE; BIOCONVERSION; BIOREACTORS; BOTTLES; CULTIVATION; DESIGN; ESTERIFICATION; EXPERIMENTS; GLYCOSYLATION; MEASUREMENT THEORY; OPTIMAL SYSTEMS; OPTIMIZATION; QUALITY CONTROL; SPIN DYNAMICS; TUBES (COMPONENTS);

CELL CULTURE;

POLYSACCHARIDE; RECOMBINANT ERYTHROPOIETIN; ERYTHROPOIETIN; RECOMBINANT PROTEIN;

ANALYSIS OF VARIANCE; ANIMAL; ARTICLE; BIOLOGICAL MODEL; BIOREACTOR; CELL PROLIFERATION; CHEMISTRY; CHO CELL; CRICETULUS; CULTURE TECHNIQUE; CYTOLOGY; GLYCOSYLATION; HAMSTER; HUMAN; INSTRUMENTATION; METABOLISM; METHODOLOGY; REPRODUCIBILITY; SYSTEMS BIOLOGY; CHO CELL LINE; DEVICES; PROCEDURES;

ANALYSIS OF VARIANCE; ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CHO CELLS; CRICETINAE; CRICETULUS; EPOETIN ALFA; GLYCOSYLATION; HUMANS; MODELS, BIOLOGICAL; POLYSACCHARIDES; REPRODUCIBILITY OF RESULTS; SYSTEMS BIOLOGY; ERYTHROPOIETIN; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS;

EID: 77954734842     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.390     Document Type: Article

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