Efficient removal of DNA from proteomic samples prior to two-dimensional map analysis

Journal of Chromatography A

Volumn 1216, Issue 17, 2009, Pages 3606-3612

  Antonioli, Paolo ^a   Bachi, Angela ^b   Fasoli, Elisa ^a   Righetti, Pier Giorgio ^a  

^a POLITECNICO DI MILANO   (Italy)

^b SAN RAFFAELE HOSPITAL   (Italy)

Author keywords

Bacterial proteomics; DNA extraction; Nuclear extracts; Two dimensional maps

Indexed keywords

ALKALINE PH; BACTERIAL PROTEOMICS; CO PRECIPITATIONS; COULOMBIC REPULSIONS; DNA EXTRACTION; DNA SAMPLES; DNA-PROTEIN COMPLEXES; EUKARYOTIC CELLS; LYSATES; NEUTRAL PH; NUCLEAR EXTRACTS; PH VALUES; PROTEIN PRECIPITATIONS; PROTEIN PREPARATIONS; PROTEIN SAMPLES; PROTEIN-DNA COMPLEXES; PROTEOME ANALYSIS; SUB-CELLULAR; TWO-DIMENSIONAL MAPS; TWO-PHASE EXTRACTIONS; TWODIMENSIONAL (2D) MAPS;

ACETONE; BACTERIOLOGY; DNA; ESCHERICHIA COLI; GELATION; MOLECULAR BIOLOGY; NUCLEIC ACIDS; PH EFFECTS; PROTEINS; TWO DIMENSIONAL;

GENES;

ACETONE; BACTERIAL DNA; CHLOROFORM; DEOXYRIBONUCLEASE; DNA; ISOPENTYL ALCOHOL; METHANOL; NUCLEAR PROTEIN; PHENOL; PROTEOME;

ALKALINITY; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; DNA PROTEIN COMPLEX; ESCHERICHIA COLI; HUMAN; HUMAN CELL; MASS SPECTROMETRY; NONHUMAN; PH; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BACTERIAL PROTEINS; CELL LINE, TUMOR; CELL NUCLEUS; CHEMICAL FRACTIONATION; CHLOROFORM; DNA; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENDODEOXYRIBONUCLEASES; ENDORIBONUCLEASES; ESCHERICHIA COLI; HUMANS; HYDROGEN-ION CONCENTRATION; MASS SPECTROMETRY; PENTANOLS; PHENOL; PRECIPITATION; PROTEINS; PROTEOMICS; REPRODUCIBILITY OF RESULTS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 63249097106     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2008.11.053     Document Type: Article

References (30)

1. Anderson L.N., and Anderson N.G. Mol. Cell. Proteomics 1 (2002) 845
2. Hamdan H., and Righetti P.G. Proteomics Today (2005), Wiley-Interscience, Hoboken p. 346
3. Righetti P.G. Isoelectric Focusing: Theory, Methodology and Applications (1983), Elsevier, Amsterdam
4. Righetti P.G. Immobilized pH Gradients: Theory and Methodology (1990), Elsevier, Amsterdam
5. Damerval C., De Vienne D., Zivy M., and Thiellement H. Electrophoresis 7 (1986) 53
6. Mastro R., and Hall M. Anal. Biochem. 273 (1999) 313
7. Westermeier R., and Naven T. Proteomics in Practice (2002), Wiley-VCH, Weinheim p. 19
8. Glass W.F., Briggs R.C., and Hnilica L.S. Science 211 (1981) 70
9. Galante E.G., Caravaggio T., and Righetti P.G. Biochim. Biophys. Acta 442 (1976) 309
10. Gianazza E., and Righetti P.G. Biochim. Biophys. Acta 540 (1978) 357
11. O'Farrell P.H. J. Biol. Chem. 250 (1975) 4007
12. Sanders M.M., Groppi V.E., and Browning E.T. Anal. Biochem. 103 (1980) 157
13. Willard K.E., Giometti C., Anderson N.L., O'Connor T.E., and Anderson N.G. Anal. Biochem. 100 (1979) 289
14. Mohberg J., and Rusch H.P. Arch. Biochem. Biophys. 134 (1969) 577
15. Yoshida M., and Shimura K. Biochim. Biophys. Acta 263 (1972) 690
16. Rabilloud T. Electrophoresis 17 (1996) 813
17. Goodwin G.M., and Dahlberg A.E. In: Rickwood D., and Hames B.D. (Eds). Gel Electrophoresis of Nucleic Acids (1982), IRL Press, Oxford 199
18. Osborn M.J., and Munson R. Methods Enzymol. 31 (1974) 642
19. Gianazza E., Giacon P., Sahlin B., and Righetti P.G. Electrophoresis 6 (1985) 53
20. Simò C., Bachi A., Cattaneo A., Guerrier L., Fortis F., Boschetti E., Podtelejnikov A., and Righetti P.G. Anal. Chem. 80 (2008) 3547
21. Gersten D.M., and Zapolski E.J. In: Chrambach A., Dunn M.J., and Radola B.J. (Eds). Advances in Electrophoresis (1991), VCH, Weinheim 49
22. Dreger M. Eur. J. Biochem. 270 (2003) 589
23. Huber L.A., Pfaller K., and Vietor I. Circ. Res. 92 (2003) 962
24. Stannard C., Brown L.R., and Godovac-Zimmermann J. Curr. Proteomics 1 (2004) 13
25. Righetti P.G., Castagna A., Antonioli P., Cecconi D., Campostrini N., and Righetti S.C. Expert Rev. Proteomics 2 (2005) 215
26. Drysdale J.W., and Righetti P.G. Biochemistry 11 (1972) 4044
27. Gianazza E., and Righetti P.G. J. Chromatogr. 193 (1980) 1
28. Pietrogrande M.C., Marchetti N., Dondi F., and Righetti P.G. Electrophoresis 24 (2003) 217
29. Campostrini N., Areces L.B., Rappsilber J., Pietrogrande M.C., Dondi F., Pastorino F., Ponzoni M., and Righetti P.G. Proteomics 5 (2005) 2385
30. Gianazza E., Astrua-Testori S., Caccia P., Giacon P., Quaglia L., and Righetti P.G. Electrophoresis 7 (1986) 76