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Volumn 47, Issue 3, 2010, Pages 91-96

A new Yarrowia lipolytica expression system: An efficient tool for rapid and reliable kinetic analysis of improved enzymes

Author keywords

Enzyme; Protein engineering; Protein expression; Yarrowia lipolytica

Indexed keywords

A-COEFFICIENT; ACTIVE SITE; CONFIDENCE INTERVAL; ENANTIOMER DISCRIMINATION; EXPRESSION CASSETTE; EXPRESSION LEVELS; EXPRESSION SYSTEM; FALSE POSITIVE; HIGH REPRODUCIBILITY; KINETIC ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION LEVELS; PROTEIN EXPRESSIONS; PROTEIN PURIFICATION; STANDARD DEVIATION; TRANSFORMANTS; Y. LIPOLYTICA; YARROWIA LIPOLYTICA;

EID: 77954659660     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2010.06.001     Document Type: Article
Times cited : (17)

References (25)
  • 2
    • 3543076929 scopus 로고    scopus 로고
    • Enantioselective biocatalysis optimized by directed evolution
    • Jaeger K.E., Eggert T. Enantioselective biocatalysis optimized by directed evolution. Curr Opin Biotechnol 2004, 15:305-313.
    • (2004) Curr Opin Biotechnol , vol.15 , pp. 305-313
    • Jaeger, K.E.1    Eggert, T.2
  • 3
    • 3543087310 scopus 로고    scopus 로고
    • Trends and innovations in industrial biocatalysis for the production of fine chemicals
    • Panke S., Held M., Wubbolts M. Trends and innovations in industrial biocatalysis for the production of fine chemicals. Curr Opin Biotechnol 2004, 15:272-279.
    • (2004) Curr Opin Biotechnol , vol.15 , pp. 272-279
    • Panke, S.1    Held, M.2    Wubbolts, M.3
  • 4
    • 0036843758 scopus 로고    scopus 로고
    • Microbial/enzymatic synthesis of chiral intermediates for pharmaceuticals
    • Patel R.N. Microbial/enzymatic synthesis of chiral intermediates for pharmaceuticals. Enzyme Microb Technol 2002, 31:804-826.
    • (2002) Enzyme Microb Technol , vol.31 , pp. 804-826
    • Patel, R.N.1
  • 5
    • 0042432086 scopus 로고    scopus 로고
    • Directed evolution of industrial enzymes: an update
    • Cherry J.R., Fidantsef A.L. Directed evolution of industrial enzymes: an update. Curr Opin Biotechnol 2003, 14:438-443.
    • (2003) Curr Opin Biotechnol , vol.14 , pp. 438-443
    • Cherry, J.R.1    Fidantsef, A.L.2
  • 6
    • 0033908030 scopus 로고    scopus 로고
    • Designed evolution of enzymatic properties
    • Petrounia I.P., Arnold F.H. Designed evolution of enzymatic properties. Curr Opin Biotechnol 2000, 11:325-330.
    • (2000) Curr Opin Biotechnol , vol.11 , pp. 325-330
    • Petrounia, I.P.1    Arnold, F.H.2
  • 7
    • 1842531448 scopus 로고    scopus 로고
    • Recent progress in biocatalyst discovery and optimization
    • Robertson D.E., Steer B.A. Recent progress in biocatalyst discovery and optimization. Curr Opin Chem Biol 2004, 8:141-149.
    • (2004) Curr Opin Chem Biol , vol.8 , pp. 141-149
    • Robertson, D.E.1    Steer, B.A.2
  • 8
    • 12344337569 scopus 로고    scopus 로고
    • Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations
    • Park S., Morley K.L., Horsman G.P., Holmquist M., Hult K., Kazlauskas R.J. Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations. Chem Biol 2005, 12:45-54.
    • (2005) Chem Biol , vol.12 , pp. 45-54
    • Park, S.1    Morley, K.L.2    Horsman, G.P.3    Holmquist, M.4    Hult, K.5    Kazlauskas, R.J.6
  • 9
    • 0141888961 scopus 로고    scopus 로고
    • An overview of high-throughput screening systems for enantioselective enzymatic transformations
    • Reetz M.T. An overview of high-throughput screening systems for enantioselective enzymatic transformations. Methods Mol Biol 2003, 230:259-282.
    • (2003) Methods Mol Biol , vol.230 , pp. 259-282
    • Reetz, M.T.1
  • 11
    • 0037048809 scopus 로고    scopus 로고
    • High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris
    • Boettner M., Prinz B., Holz C., Stahl U., Lang C. High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris. J Biotechnol 2002, 99:51-62.
    • (2002) J Biotechnol , vol.99 , pp. 51-62
    • Boettner, M.1    Prinz, B.2    Holz, C.3    Stahl, U.4    Lang, C.5
  • 12
    • 5444226007 scopus 로고    scopus 로고
    • Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena
    • Weis R., Luiten R., Skranc W., Schwab H., Wubbolts M., Glieder A. Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena. FEMS Yeast Res 2004, 5:179-189.
    • (2004) FEMS Yeast Res , vol.5 , pp. 179-189
    • Weis, R.1    Luiten, R.2    Skranc, W.3    Schwab, H.4    Wubbolts, M.5    Glieder, A.6
  • 14
    • 1842530397 scopus 로고    scopus 로고
    • Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review
    • Madzak C., Gaillardin C., Beckerich J.-M. Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. J Biotechnol 2004, 109:63-81.
    • (2004) J Biotechnol , vol.109 , pp. 63-81
    • Madzak, C.1    Gaillardin, C.2    Beckerich, J.-M.3
  • 16
    • 34548151199 scopus 로고    scopus 로고
    • A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica
    • Bordes F., Fudalej F., Dossat V., Nicaud J.M., Marty A. A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica. J Microbiol Methods 2007, 70:493-502.
    • (2007) J Microbiol Methods , vol.70 , pp. 493-502
    • Bordes, F.1    Fudalej, F.2    Dossat, V.3    Nicaud, J.M.4    Marty, A.5
  • 17
    • 0028284967 scopus 로고
    • Ylt1, a highly repetitive retrotransposon in the genome of the dimorphic fungus Yarrowia lipolytica
    • Schmid-Berger N., Schmid B., Barth G. Ylt1, a highly repetitive retrotransposon in the genome of the dimorphic fungus Yarrowia lipolytica. J Bacteriol 1994, 176:2477-2482.
    • (1994) J Bacteriol , vol.176 , pp. 2477-2482
    • Schmid-Berger, N.1    Schmid, B.2    Barth, G.3
  • 18
    • 0034891767 scopus 로고    scopus 로고
    • Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization
    • Mauersberger S., Wang H.J., Gaillardin C., Barth G., Nicaud J.M. Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization. J Bacteriol 2001, 183:5102-5109.
    • (2001) J Bacteriol , vol.183 , pp. 5102-5109
    • Mauersberger, S.1    Wang, H.J.2    Gaillardin, C.3    Barth, G.4    Nicaud, J.M.5
  • 19
    • 8144225468 scopus 로고    scopus 로고
    • New efficient lipase from Yarrowia lipolytica for the resolution of 2-bromo-arylacetic acid esters
    • Guieysse D., Sandoval G., Faure L., Nicaud J.-M., Monsan P., Marty A. New efficient lipase from Yarrowia lipolytica for the resolution of 2-bromo-arylacetic acid esters. Tetrahedron: Asymmetr 2004, 15:3539-3543.
    • (2004) Tetrahedron: Asymmetr , vol.15 , pp. 3539-3543
    • Guieysse, D.1    Sandoval, G.2    Faure, L.3    Nicaud, J.-M.4    Monsan, P.5    Marty, A.6
  • 20
    • 70349559774 scopus 로고    scopus 로고
    • Improvement of Yarrowia lipolytica Lipase Enantioselectivity by Using Mutagenesis Targeted to the Substrate Binding Site
    • Bordes F., Cambon E., Dossat-Letisse V., Andre I., Croux C., Nicaud J.M., et al. Improvement of Yarrowia lipolytica Lipase Enantioselectivity by Using Mutagenesis Targeted to the Substrate Binding Site. Chembiochem 2009, 10:1705-1713.
    • (2009) Chembiochem , vol.10 , pp. 1705-1713
    • Bordes, F.1    Cambon, E.2    Dossat-Letisse, V.3    Andre, I.4    Croux, C.5    Nicaud, J.M.6
  • 21
    • 13544261563 scopus 로고    scopus 로고
    • Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytica
    • Fickers P., Fudalej F., Le Dall M.T., Casaregola S., Gaillardin C., Thonart P., et al. Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytica. Fungal Genet Biol 2005, 42:264-274.
    • (2005) Fungal Genet Biol , vol.42 , pp. 264-274
    • Fickers, P.1    Fudalej, F.2    Le Dall, M.T.3    Casaregola, S.4    Gaillardin, C.5    Thonart, P.6
  • 22
    • 0028287703 scopus 로고
    • Multiple-copy integration in the yeast Yarrowia lipolytica
    • Le Dall M.T., Nicaud J.M., Gaillardin C. Multiple-copy integration in the yeast Yarrowia lipolytica. Curr Genet 1994, 26:38-44.
    • (1994) Curr Genet , vol.26 , pp. 38-44
    • Le Dall, M.T.1    Nicaud, J.M.2    Gaillardin, C.3
  • 25
    • 0031657285 scopus 로고    scopus 로고
    • Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Kluyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica
    • Muller S., Sandal T., Kamp-Hansen P., Dalboge H. Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Kluyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast 1998, 14:1267-1283.
    • (1998) Yeast , vol.14 , pp. 1267-1283
    • Muller, S.1    Sandal, T.2    Kamp-Hansen, P.3    Dalboge, H.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.