High-yield extraction of periplasmic asparaginase produced by recombinant Pichia pastoris harbouring the Saccharomyces cerevisiae ASP3 gene

Enzyme and Microbial Technology

Volumn 47, Issue 3, 2010, Pages 71-76

  Ferrara, Maria Antonieta ^a   Severino, Neuza M Bonomo ^b   Valente, Richard H ^a   Perales, Jonas ^a   Bon, Elba P S ^b  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

ASP3 gene; Asparaginase production; Periplasmic asparaginase extraction; Pichia pastoris; Yeast periplasmic enzyme

Indexed keywords

ASP3 GENE; ASPARAGINASE PRODUCTION; PERIPLASMIC ENZYMES; PICHIA PASTORIS;

ENZYMES; ETHANOL; PHOSPHATES; POTASSIUM; THAWING; YEAST;

EXTRACTION;

ALCOHOL; ASPARAGINASE; CYSTEINE;

ALKALINIZATION; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SYNTHESIS; FREEZE THAWING; FUNGAL STRAIN; MASS SPECTROMETRY; NONHUMAN; PICHIA PASTORIS; RECOMBINANT GENE; RNA EXTRACTION; SACCHAROMYCES CEREVISIAE; YEAST CELL;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 77954657006     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2010.05.001     Document Type: Article

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