Pitfalls in the rapid diagnosis of positive blood culture

Reviews and Research in Medical Microbiology

Volumn 21, Issue 3, 2010, Pages 39-43

  Wang, Pei ^a   Yang, Zheng Tao ^a   He, Yong Gui ^a   Shu, Chun Ming ^a  

^a First People's Hospital of Jingmen   (China)

Author keywords

bacteraemia; blood culture; microbiological laboratory; sepsis

Indexed keywords

ANTIBIOTIC SENSITIVITY; BACTEREMIA; BACTERIAL STRAIN; BACTERIUM CONTAMINATION; BACTERIUM IDENTIFICATION; BLOOD CULTURE; IMMUNOLOGICAL TECHNIQUE; INFRARED SPECTROSCOPY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROBIOLOGICAL EXAMINATION; NONHUMAN; PRACTICE GUIDELINE; PRIORITY JOURNAL; REVIEW; SEPSIS;

EID: 77954653480     PISSN: 0954139X     EISSN: None     Source Type: Journal    
DOI: 10.1097/MRM.0b013e32833bb953     Document Type: Review

References (33)

1. Badillo AT, Sarani B, Evans SR. Optimizing the use of blood cultures in the febrile postoperative patient. J Am Coll Surg 2002; 194:477-487.
2. Savinelli T, Parenteau S, Mermel LA. What happens when automated blood culture instrument detect growth but there are no technologists in the microbiology laboratory? Diagn Microbiol Infect Dis 2004; 48:173-174.
3. Fitzpatrick F, Turley M, Humphreys H, Smyth E. An after-hours clinical liaison blood culture service - is it worth it? Clin Microbiol Infect 2004; 10:917-921.
4. Eveillard M, Lemarié C, Cottin J, Hitoto H, Mahaza C, Kempf M, Joly-Guillou ML. Assessment of the usefulness of performing bacterial identification and antimicrobial susceptibility testing 24 h a day in a clinical microbiology laboratory. Clin Microbiol Infect 2009. doi:10.1111/j.1469-0691. 2009.03044.x
5. Barenfanger J, Graham DR, Kolluri L, Sangwan G, Lawhorn J, Drake CA, et al. Decreased mortality associated with prompt Gramstaining of blood cultures.AmJ Clin Pathol 2008; 130:870-876.
6. Munson EL, Diekema DJ, Beekmann SE, Chapin KC, Doern GV. Detection and treatment of bloodstream infection: laboratory reporting and antimicrobial management. J Clin Microbiol 2003; 41:495-497.
7. Beekmann SE, Diekema DJ, Chapin KC, Doern GV. Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol 2003; 41:3119-3125.
8. Petkar HM, Breathnach AS. Telephoning of interim blood culture results: a regional survey. J Clin Pathol 2008; 61:1142-1143.
9. Murdoch DR, Greenlees RL. Rapid identification of Staphylococcus aureus from Bact/Alert blood culture bottles by direct Gram stain characteristics. J Clin Pathol 2004; 57:199-201.
10. Harrington A, McCourtney K, Nowowiejski D, Limaye A. Differentiation of Candida albicans from nonalbicans yeast directly from blood cultures by Gram stain morphology. Eur J Clin Microbiol Infect Dis 2007; 26:325-329.
11. Strand CL. Positive blood cultures: can we always trust the Gram stain? Am J Clin Pathol 2006; 126:671-672.
12. Saito T, Aoki Y, Mori Y, Kohi F. Blood culture examinations at a community hospital without a microbiology laboratory: using an automated blood culture system and performing a Gram stain on positive culture bottles in the institution. J Infect Chemother 2004; 10:239-241.
13. Qian Q, Eicheberlberger K, Eirby JE. Rapid identification of Staphylococcus aureus in blood cultures by use of the direct tube coagulase test. J Clin Microbiol 2007; 45:2267-2269.
14. Chapin K, Musgnug M. Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures. J Clin Microbiol 2003; 41:4324-4327.
15. Petti CA, Woods CW, Reller LB. Streptococcus pneumoniae antigen test using positive blood culture bottles as an alternative method to diagnose pneumococcal bacteremia. J Clin Microbiol 2005; 43:2510-2512.
16. Chapin KC, Musgnug MC. Evaluation of penicillin binding protein 2a latex agglutination assay for identification of methicillin- resistant Staphylococcus aureus directly from blood cultures. J Clin Microbiol 2004; 42:1283-1284.
17. Hansen DS, Jensen AG, Nørskov-Lauritsen N, Skov R, Bruun B. Direct identification and susceptibility testing of enteric bacilli from positive blood cultures using VITEK (GNIR/GNS-GA). Clin Microbiol Infect 2002; 8:38-44.
18. Chen JR, Lee SY, Yang BH, Lu JJ. Rapid identification and susceptibility testing using the VITEK 2 system using culture fluids from positive BacT/ALERT blood cultures. J Microbiol Immunol Infect 2008; 41:259-264.
19. Tavares A, Inácio J, Melo-Cristino J, Couto I. Use of fluorescence in situ hybridization for rapid identification of staphylococci in blood culture samples collected in a Portuguese hospital. J Clin Microbiol 2008; 46:3097-3100.
20. Wang P. Simultaneous detection and differentiation of Staphylococcus species in blood cultures using fluorescence in situ hybridization. Med Princ Pract 2010; 19:218-221.
21. Peters RP, Savelkoul PH, Simoons-Smit AM, Danner SA, Vandenbroucke-Grauls CM, van Agtmael MA. Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J Clin Microbiol 2006; 44:119-123.
22. Gherna M, Merz WG. Identification of Candida albicans and Candida glabrata within 1.5 h directly from positive blood culture bottles with a shortened peptide nucleic acid fluorescence in situ hybridization protocol. J Clin Microbiol 2009; 47:247-248.
23. Stevenson LG, Drake SK, Murray PR. Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2010; 48:444-447.
24. Kerremans JJ, Goessens WH, Verbrugh HA, Vos MC. Accuracy of identification and susceptibility results by direct inoculation of Vitek 2 cards from positive BACTEC cultures. Eur J Clin Microbiol Infect Dis 2004; 23:892-898.
25. Edelmann A, Pietzcker T, Wellinghausen N. Comparison of direct disk diffusion and standard microtitre broth dilution susceptibility testing of blood culture isolates. J Med Microbiol 2007; 56:202-207.
26. Hong T, Ndamukong J, Millett W, Kish A, Win KK, Choi YJ. Direct application of Etest to Gram-positive cocci from blood cultures: quick and reliable minimum inhibitory concentration data. Diagn Microbiol Infect Dis 1996; 25:21-25.
27. Palasubramaniam S, Muniandy S, Navaratnam P. Rapid detection of ESBL-producing Klebsiella pneumoniae in blood cultures by fluorescent in-situ hybridization. J Microbiol Methods 2008; 72:107-109.
28. Levi K, Towner KJ. Detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood with the EVIGENE MRSA detection kit. J Clin Microbiol 2003; 41:3890-3892.
29. Wang P. Recent advances in the rapid diagnosis of various infectious diseases by PNA FISH. Rev Med Microbiol 2009; 20:56-62.
30. Hall KK, Lyman JA. Updated review of blood culture contamination. Clin Microbiol Rev 2006; 19:788-802.
31. Waltzman ML, Harper M. Financial and clinical impact of false positive blood culture results. Clin Infect Dis 2001; 33:296-299.
32. Beekmann SE, Diekema DJ, Doern GV. Determining the clinical significance of coagulase-negative staphylococci isolated from blood cultures. Infect Control Hosp Epidemiol 2005; 26:559-566.
33. Kassis C, Rangaraj G, Jiang Y, Hachem RY, Raad I. Differentiating culture samples representing coagulase-negative staphylococcal bacteremia from those representing contamination by use of time-to-positivity and quantitative blood culture methods. J Clin Microbiol 2009; 47:3255-3260.