Use of fluorescence in situ hybridization for rapid identification of staphylococci in blood culture samples collected in a Portuguese hospital

Journal of Clinical Microbiology

Volumn 46, Issue 9, 2008, Pages 3097-3100

  Tavares, Ana ^a,b,d   Inácio, João ^b   Melo Cristino, José ^c   Couto, Isabel ^a,b  

^a INSTITUTO DE HIGIENE E MEDICINA TROPICAL   (Portugal)

^b UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^c UNIVERSITY OF LISBON   (Portugal)

^d INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM IDENTIFICATION; BLOOD CULTURE; BLOOD SAMPLING; COAGULASE NEGATIVE STAPHYLOCOCCUS; FLUORESCENCE IN SITU HYBRIDIZATION; HOSPITAL; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PORTUGAL; PRIORITY JOURNAL;

CROSS INFECTION; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; PORTUGAL; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS;

STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 53649087527     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00910-08     Document Type: Article

References (17)

1. Amann, R., L. Krumholz, and D. A. Stahl. 1990. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172:762-770.
2. Biedenbach, D. J., G. J. Moet, and R. N. Jones. 2004. Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002). Diagn. Microbiol. Infect. Dis. 50:59-69.
3. Brosius, J., T. J. Dull, D. D. Sleeter, and F. Noller. 1981. Gene organization and primary structure of ribosomal RNA operon from Escherichia coli. J. Mol. Biol. 148:107-127.
4. Chapin, K., and M. Musgnug. 2003. Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures. J. Clin. Microbiol. 41:4324-4327.
5. Forrest, G. N., S. Mehta, E. Weekes, D. P. Lincalis, J. K. Johnson, and R. A. Venezia. 2006. Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J. Antimicrob. Chemother. 58:154-158.
6. Fuchs, B. M., G. Wallner, W. Beisker, I. Schwippl, W. Ludwig, and R. Amann. 1998. Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl. Environ. Microbiol. 64:4973-4982.
7. González, V., E. Padilla, M. Gimenez, C. Vilaplana, A. Perez, G. Fernandez, M. D. Quesada, M. A. Pallares, and V. Ausina. 2004. Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH. Eur. J. Clin. Microbiol. Infect. Dis. 23:396-398.
8. Hall, K. K., and J. A. Lyman. 2006. Updated review of blood culture contamination. Clin. Microbiol. Rev. 19:788-802.
9. Hogardt, M., K. Trebesius, A. M. Geiger, M. Hornef, J. Rosenecker, and J. Heesemann. 2000. Specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. J. Clin. Microbiol. 38:818-825.
10. Kempf, V. A., K. Trebesius, and I. B. Autenrieth. 2000. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J. Clin. Microbiol. 38:830-838.
11. Krimmer, V., H. Merkert, C. von Eiff, M. Frosch, J. Eulert, J. F. Lohr, J. Hacker, and W. Ziebuhr. 1999. Detection of Staphylococcus aureus and Staphylococcus epidermidis in clinical samples by 16S rRNA-directed in situ hybridization. J. Clin. Microbiol. 37:2667-2673.
12. Miragaia, M., I. Couto, S. F. Pereira, K. G. Kristinsson, H. Westh, J. O. Jarlov, J. Carriço, J. Almeida, I. Santos-Sanches, and H. de Lencastre. 2002. Molecular characterization of methicillin-resistant Staphylococcus epidermidis clones: evidence of geographic dissemination. J. Clin. Microbiol. 40:430-438.
13. Oliveira, K., S. M. Brecher, A. Durbin, D. S. Shapiro, D. R. Schwartz, P. C. De Girolami, J. Dakos, G. W. Procop, D. Wilson, C. S. Hanna, G. Haase, H. Peltroche-Llacsahuanga, K. C. Chapin, M. C. Musgnug, M. H. LeShoemaker, and H. Stender. 2003. Direct identification of Staphylococcus aureus from positive blood culture bottles. J. Clin. Microbiol. 41:889-891.
14. Peters, R. P., P. H. Savelkoul, A. M. Simoons-Smit, S. A. Danner, C. M. Vandenbroucke-Grauls, and M. A. van Agtmael. 2006. Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J. Clin. Microbiol. 44:119-123.
15. Santos Sanches, I., M. Aires de Sousa, L. Sobral, I. Calheiros, L. Felicio, I. Pedra, and H. de Lencastre. 1995. Multidrug-resistant Iberian epidemic clone of methicillin-resistant Staphylococcus aureus endemic in a hospital in northern Portugal. Microb. Drug Resist. 1:299-306.
16. Santos Sanches, I., R. Mato, H. de Lencastre, and A. Tomasz. 2000. Patterns of multidrug resistance among methicillin-resistant hospital isolates of coagulase-positive and coagulase-negative staphylococci collected in the international multicenter study RESIST in 1997 and 1998. Microb. Drug Resist. 6:199-211.
17. Wisplinghoff, H., T. Bischoff, S. M. Tallent, H. Seifert, R. P. Wenzel, and M. B. Edmond. 2004. Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin. Infect. Dis. 39:309-317.