Simultaneous detection and differentiation of staphylococcus speciesin blood cultures using fluorescence in situ hybridization

Medical Principles and Practice

Volumn 19, Issue 3, 2010, Pages 218-221

  Wang, Pei ^a  

^a First People's Hospital of Jingmen   (China)

Author keywords

Bacteremia; Fluorescence in situ hybridization; Staphylococcus

Indexed keywords

FLUOROCHROME; ISOTHIOCYANIC ACID; RNA 16S;

ARTICLE; BACTERIUM DETECTION; BLOOD CULTURE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FLUORESCENCE IN SITU HYBRIDIZATION; NONHUMAN; OLIGONUCLEOTIDE PROBE; STAPHYLOCOCCUS AUREUS; STRAIN DIFFERENCE;

BACTERIOLOGICAL TECHNIQUES; BLOOD SPECIMEN COLLECTION; COAGULASE; FLUORESCENT DYES; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS;

EID: 77950545640     PISSN: 10117571     EISSN: None     Source Type: Journal    
DOI: 10.1159/000285294     Document Type: Article

References (9)

1. Martin GS, Mannino DM, Eaton S, Moss M: The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 2003; 348: 1546-1554. (Pubitemid 36437931)
2. Forrest GN, Mehta S, Weeks E, Lincalis DP, Johnson JK, Venezia RA: Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J Antimicrob Chemother 2006; 58: 154-158. (Pubitemid 44294999)
3. Kempf VA, Trebesius K, Autenrieth IB: Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J Clin Microbiol 2000; 38: 830-838. (Pubitemid 30082689)
4. Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux R, Stahl DA: Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 1990; 56: 1919-1925. (Pubitemid 20202165)
5. Jansen GJ, Mooibroek M, Idema J, Harmsen HJ, Welling GW, Degener JE: Rapid identification of bacteria in blood cultures by using fluorescently labeled oligonucleotide probes. J Clin Microbiol 2000; 38: 814-817. (Pubitemid 30082686)
6. Peters RP, Savelkoul PH, Simoons-Smit AM, Danner SA, Vandenbroucke-Grauls CM, Van Agtmarl MA: Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J Clin Microbiol 2006; 44: 119-123. (Pubitemid 43100589)
7. González V, Padilla E, Giménez M, Vilaplana C, Pérez A, Fernández G, Quesada MD, Pallarés MA, Ausina V: Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH. Eur J Clin Microbiol Infect Dis 2004; 23: 396-398. (Pubitemid 38797723)
8. Oliveira K, Procop GW, Wilson D, Coull J, Stender H: Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes. J Clin Microbiol 2002; 40: 247-251. (Pubitemid 34027631)
9. Cerqueira L, Azevedo NF, Almeida C, Jardim T, Keevil CW, Vieira MJ: DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH). Int J Mol Sci 2008; 9: 1944-1960.