Rapid detection of ESBL-producing Klebsiella pneumoniae in blood cultures by fluorescent in-situ hybridization

Journal of Microbiological Methods

Volumn 72, Issue 1, 2008, Pages 107-109

  Palasubramaniam, Selvi ^a   Muniandy, Sekaran ^a   Navaratnam, Parasakthi ^b  

^a UNIVERSITY OF MALAYA   (Malaysia)

^b MONASH UNIVERSITY MALAYSIA   (Malaysia)

Author keywords

Blood culture; FISH; K. pneumoniae; SHV type ESBL

Indexed keywords

EXTENDED SPECTRUM BETA LACTAMASE;

ARTICLE; BACTERIUM IDENTIFICATION; BLOOD CULTURE; CONTROLLED STUDY; DIAGNOSTIC TEST; FLUORESCENCE IN SITU HYBRIDIZATION; GENE; KLEBSIELLA PNEUMONIAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

BETA-LACTAM RESISTANCE; BETA-LACTAMASES; BLOOD; CULTURE MEDIA; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; OLIGONUCLEOTIDE PROBES; PNEUMONIA, BACTERIAL; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE; KLEBSIELLA PNEUMONIAE;

EID: 37349127765     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.10.008     Document Type: Article

References (7)

1. Fluit A.C., and Schmitz F.-J. The use of molecular techniques to detect antimicrobial resistance in clinical bacterial isolates. Microbiol. Today. 28 (2001) 14-15
2. Jansen G.J., Mooibroek M., Idema J., Harmsen H.J.M., Welling G.W., and Degener J.E. Rapid identification of bacteria in blood cultures by using fluorescently labeled oligonucleotide probes. J. Clin. Microbiol. 38 (2000) 814-817
3. Jarlier V., Nicolas M.H., Fournier G., and Philippon A. Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactams in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev. Infect. Dis. 10 (1988) 867-878
4. Kim J., and Lee H.J. Rapid discriminatory detection of genes coding for SHV β-lactamases by ligase chain reaction. Antimicrob. Agents Chemother. 44 (2000) 1860-1864
5. Medeiros A.A. Evolution and dissemination of β-lactamases accelerated by generations of β-lactam antibiotics. Clin. Infect. Dis. 24 (1997) 19-45
6. Pitout J.D.D., Nordmann P., Laupland K.B., and Poirel L. Emergence of Enterobacteriaceae producing extended-spectrum β-lactamases (ESBLs) in the community. J Antimicrob. Chemother. 56 (2005) 52-59
7. Randegger C.C., and Hachler H. Real-time PCR and melting curve analysis for reliable and rapid detection of SHV extended-spectrum beta-lactamases. Antimicrob. Agents Chemother. 45 (2001) 1730-1736