Biological and genetic characterization of Cryptosporidium spp. and Giardia duodenalis isolates from five hydrographical basins in northern Portugal

Korean Journal of Parasitology

Volumn 48, Issue 2, 2010, Pages 105-111

  Almeida, André ^a,b   Moreira, Maria João ^a,b   Soares, Sónia ^a,b   de Lurdes Delgado, Maria ^a,b   Figueiredo, João ^a,b   Silva, Elisabete ^a,b   Castro, António ^a,b   da Costa, Alexandra Viana ^b,c   da Costa, José Manuel Correia ^a,b  

^a NATIONAL INSTITUTE OF HEALTH   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

^c UNIVERSITY INSTITUTE OF HEALTH SCIENCES IUCS   (Portugal)

Author keywords

Cryptosporidium spp.; Giardia duodenalis; Portugal; Public health; Risk assessment; Surface water

Indexed keywords

PROTOZOAL RNA; RIVER WATER; RNA 18S; SURFACE WATER; PROTOZOAL DNA; RIBOSOME DNA;

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM ANDERSONI; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM MURIS; CRYPTOSPORIDIUM PARVUM; GENOTYPE; GIARDIA DUODENALIS; NONHUMAN; OOCYST; PARASITE ISOLATION; PORTUGAL; PROTOZOAL GENETICS; RIVER BASIN; RNA SEQUENCE; WATER CONTAMINATION; CHEMISTRY; CLASSIFICATION; DNA SEQUENCE; GENETICS; GEOGRAPHY; GIARDIA INTESTINALIS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; POLYMERASE CHAIN REACTION; PREVALENCE; RNA GENE;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM ANDERSONI; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM MURIS; CRYPTOSPORIDIUM PARVUM; GIARDIA; GIARDIA INTESTINALIS; PROTOZOA;

CRYPTOSPORIDIUM PARVUM; DNA, PROTOZOAN; DNA, RIBOSOMAL; GENES, RRNA; GEOGRAPHY; GIARDIA LAMBLIA; HUMANS; POLYMERASE CHAIN REACTION; PORTUGAL; PREVALENCE; RNA, PROTOZOAN; RNA, RIBOSOMAL, 18S; SEQUENCE ANALYSIS, DNA; WATER MICROBIOLOGY;

EID: 77954444243     PISSN: 00234001     EISSN: 17380006     Source Type: Journal    
DOI: 10.3347/kjp.2010.48.2.105     Document Type: Article

References (30)

1. Hunter PR, Thompson RC. The zoonotic transmission of Giardia and Cryptosporidium. Int J Parasitol 2005; 35: 1181-1190.
2. Fayer R, Morgan U, Upton SJ. Epidemiology of Cryptosporidium: transmission, detection and identification. Int J Parasitol 2000; 30: 1305-1322.
3. Adam RD. Biology of Giardia lamblia. Clin Microbiol Rev 2001; 14: 447-475.
4. Caccio SM, Ryan U. Molecular epidemiology of giardiasis. Mol Biochem Parasitol 2008; 160: 75-80.
5. Fayer R. Taxonomy and species delimitation in Cryptosporidium. Exp Parasitol 2009; 124: 90-97.
6. Smith HV, Caccio SM, Cook N, Nichols RA, Tait A. Cryptosporidium and Giardia as foodborne zoonoses. Vet Parasitol 2007; 149: 29-40.
7. Chappell CL, Okhuysen PC, Langer-Curry R, Widmer G, Akiyoshi DE, Tanriverdi S, Tzipori S. Cryptosporidium hominis: experimental challenge of healthy adults. Am J Trop Med Hyg 2006; 75: 851-857.
8. Thompson RC. Giardiasis as a re-emerging infectious disease and its zoonotic potential. Int J Parasitol 2000; 30: 1259-1267.
9. Trout JM, Santin M, Greiner EC, Fayer R. Prevalence and genotypes of Giardia duodenalis in 1-2 year old dairy cattle. Vet Parasitol 2006; 140: 217-222.
10. Caccio SM, De Giacomo M, Aulicino FA, Pozio E. Giardia cysts in wastewater treatment plants in Italy. Appl Environ Microbiol 2003; 69: 3393-3398.
11. Chauret C, Springthorpe S, Sattar S. Fate of Cryptosporidium oocysts, Giardia cysts, and microbial indicators during wastewater treatment and anaerobic sludge digestion. Can J Microbiol 1999; 45: 257-262.
12. Fayer R. Cryptosporidium: a water-borne zoonotic parasite. Vet Par-asitol 2004; 126: 37-56.
13. Almeida AA, Delgado ML, Soares SC, Castro AO, Moreira MJ, Mendonca CM, Canada NB, Da Costa JM. Genotype analysis of Giardia isolated from asymptomatic children in northern Portugal. J Eukaryot Microbiol 2006; 53: S177-S178.
14. Almeida AA, Delgado ML, Soares SC, Castro AO, Moreira MJ, Mendonca CM, Canada NB, Da Costa JM, Coelho HG. Genetic characterization of Cryptosporidium isolates from humans in northern Portugal. J Eukaryot Microbiol 2006; 53: S26-S27.
15. Alves M, Xiao L, Antunes F, Matos O. Distribution of Cryptospori-dium subtypes in humans and domestic and wild ruminants in Portugal. Parasitol Res 2006; 99: 287-292.
16. Matos O, Alves M, Xiao L, Cama V, Antunes F. Cryptosporidium felis and C. meleagridis in persons with HIV, Portugal. Emerg Infect Dis 2004; 10: 2256-2257.
17. Sousa MC, Morais JB, Machado JE, Poiares-da-Silva J. Genotyping of Giardia lamblia human isolates from Portugal by PCR-RFLP and sequencing. J Eukaryot Microbiol 2006; 53: S174-S176.
18. Lobo ML, Xiao L, Antunes F, Matos O. Occurrence of Cryptospori-dium and Giardia genotypes and subtypes in raw and treated water in Portugal. Lett Appl Microbiol 2009; 48: 732-737.
19. Mendonca C, Almeida A, Castro A, de Lurdes Delgado M, Soares S, da Costa JM, Canada N. Molecular characterization of Cryptos-poridium and Giardia isolates from cattle from Portugal. Vet Para-sitol 2007; 147: 47-50.
20. United States Environmental Protection Agency. Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. USA. United States Environmental Protection Agency. 2005.
21. McCuin RM, Clancy JL. Modifications to United States Environmental Protection Agency methods 1622 and 1623 for detection of Cryptosporidium oocysts and Giardia cysts in water. Appl Environ Microbiol 2003; 69: 267-274.
22. Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson RC, Fayer R, Lal AA. Genetic diversity within Cryp-tosporidium parvum and related Cryptosporidium species. Appl Environ Microbiol 1999; 65: 3386-3391.
23. Caccio SM, De Giacomo M, Pozio E. Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int J Parasitol 2002; 32: 1023-1030.
24. Read CM, Monis PT, Thompson RC. Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. Infect Genet Evol 2004; 4: 125-130.
25. Xiao L, Fayer R. Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission. Int J Parasitol 2008; 38: 1239-1255.
26. Castro-Hermida JA, Garcia-Presedo I, Almeida A, Gonzalez-Warleta M, Correia Da Costa JM, Mezo M. Contribution of treated wastewater to the contamination of recreational river areas with Cryptosporidium spp. and Giardia duodenalis. Water Res 2008; 42: 3528-3538.
27. Castro-Hermida JA, Almeida A, Gonzalez-Warleta M, Correia da Costa JM, Rumbo-Lorenzo C, Mezo M. Occurrence of Cryptospori-dium parvum and Giardia duodenalis in healthy adult domestic ruminants. Parasitol Res 2007; 101: 1443-1448.
28. Alves M, Xiao L, Sulaiman I, Lal AA, Matos O, Antunes F. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J Clin Microbiol 2003; 41: 2744-2747.
29. Jiang J, Alderisio KA, Singh A, Xiao L. Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Appl Environ Microbiol 2005; 71: 1135-1141.
30. Robertson LJ, Hermansen L, Gjerde BK. Occurrence of Cryptospori-dium oocysts and Giardia cysts in sewage in Norway. Appl Environ Microbiol 2006; 72: 5297-5303.