Quantitative DNA hybridization assay on a multiplexed surface microfluidic device

Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009

Volumn 1, Issue , 2010, Pages 103-111

  Prakash, Ravi ^a   Chugh, Dipankar ^a   Kaler, Karan V I S ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Dielectrophoresis (DEP); DNA hybridization assay; High throughput screening (HTS); Liquid DEP; Multiplexed; Surface microfluidic devices

Indexed keywords

DIELECTROPHORESIS; DNA HYBRIDIZATION; HIGH THROUGHPUT SCREENING (HTS); HIGH-THROUGHPUT SCREENING; MICRO-FLUIDIC DEVICES;

DNA; DROP FORMATION; ELECTROPHORESIS; FLUIDIC DEVICES; GENES; HEAT TRANSFER; LIQUIDS; MASS TRANSFER; MICROFLUIDICS; MULTIPLEXING; THROUGHPUT;

ASSAYS;

EID: 77954330897     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1115/MNHMT2009-18270     Document Type: Conference Paper

References (30)

1. Heller H.J., "DNA microarray technology: Devices, systems and applications", Annu. Rev. Biomed. Eng. Vol. 4, (2002), 129-53.
2. Hong J.W. and Quake S.R., 2003, "Integrated nanoliter systems", Nat. Biotechnology vol. 21, (2004), 1179-1183.
3. Chen L., Lee S., Lee M., Lim C., Choo J., Park J.Y., Lee S., Joo S.W., Lee K.H. and Choi Y.W., "DNA hybridization detection in a microfluidic channel using two fluorescently labelled nucleic acid probes", Biosensors and Bioelectronics vol. 23(12), (2008), 1878-1882.
4. Unger M.A., Chou H.P., Thorsen T., Scherer A. and Quake S.R, "Monolithic microfabricated valves and pumps by multilayer soft lithography". Science vol. 288, (2000), page 113-116.
5. Fenniri H. and Alvarez-Pubela R., "High-throughput screening flows along.", Nat. Chem. Biol. Vol. 3, (2007), 247-249.
6. Mere L., Bennett T., Coassin P., England P., Hamman B., Rink T., Zimmerman S. and Negulescu P., "Miniaturized FRET assays and microfluidics: key components for ultra-highthroughput screening", Drug Discov Today vol. 4, (1999), 363-369.
7. Liu J., Hansen C. and Quake S.R. "Solving the "World-to- Chip" interface problem with a microfluidic matrix", Anal. Chem. vol. 75, (2003), pp. 4718-4723.
8. Thorsen T., Maerk S.J. and Quake S.R., "Microfluidic large-scale integration", Science, vol. 298, (2002), 580-584.
9. Pollack M.G., Shenderov A.D. and Fair R.B., "Electrowetting-based actuation of droplets for integrated microfluidics", Lab Chip, vol. 7152, (2002), 96-101.
10. Jones T.B., "Liquid dielectrophoresis on the microscale", J. Electrostatics vol. 51-52, (2001), 290-299.
11. Jones T.B., Gunji M., Washizu M. and Feldman M.J., " Dielectrophoretic liquid actuation and nanoliter droplet 'formation", J. Appl. Phys vol. 89(3), (2001), 1-8.
12. Gunji M., Nakanishi H. and Washizu M., "Droplet actuation based on single-phase electrostatic excitation", Proceedings of Micro Total Analysis Systems, Malmo, pp 168- 170, 2004.
13. Chugh D. and Kaler K.V.I.S., "Integrated liquid and droplet dielectrophoresis for biochemical assays", Microfluid Nanofluid, (2009), DOI 10.1007/s 10404-009-0469-7.
14. Chugh D. and Kaler K.V.I.S., "Integrated liquid and droplet DEP for lab-on-a-chip applications", Proceedings of 12th international conference on miniaturized systems for chemistry and life sciences, San Diego, pp 733-735, 2008.
15. Prakash R., Chugh D. and Kaler K.V.I.S., "Multiplexed Liquid and Droplet DEP manipulations for HTS", Proceedings of 13th international conference on miniaturized systems for chemistry and life sciences, Jeju, Korea, 2009.
16. Pohl H.A., "Dielectrophoresis", Cambridge University Press, Cambridge, 1978.
17. Pellat H., "Mesure de la force agissant sur les dielectriques liquids non electrises places dans un champ elitrique", C R Acad Sci, Paris vol. 119, (1895), 691-694.
18. Jones T.B., "Dynamics of Dielectrophoretic Liquid Microactuation", 4th Int'l Conference on Applied Electrostatics, Dalian, China, 2001.
19. Ahmed R. and Jones T.B., "Dispensing picoliter droplets on substrates using dielectrophoresis", J. Electrostat vol. 64, (2006), 543-549.
20. Thirukumaran T.K. and Kaler K.V.I.S., "Surface microfluidics-high- speed DEP liquid actuation on planar substrates and critical factors in reliable actuation". J. Micromech Microeng vol. 17, (2007), 743-752.
21. Lord Rayleigh, "On capillary phenomena of jets", Proc. Roy. Soc. (London) vol. 27, (1879), 71-97.
22. Johansson M.K., "Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols", Methods in Molecular Biology vol. 335, (2004).
23. Didenko V.V., "DNA probes using fluorescence resonance energy transfer (FRET): designs and applications", Biotechniques vol. 31, (2001), 1106-1121.
24. Massey M, Algar W.R. and Krull U.J., "Fluorescence resonance energy transfer (FRET) for DNA biosensors: FRET pairs and Förster distances for various dye-DNA conjugates", Analytica Chemica Acta vol. 568, (2006), 181-189.
25. Marras S.A.E., Kramer F. R. and Tyagi S., "Efficiencies of Fuorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes", Nucleic Acids Research vol. 30, (2002), No. 21 e122.
26. Bemacchi S. and Mely Y., "Exciton interaction in molecular beacons: a sensitive sensor for short-range modifications of the nucleic acid structure", Nucleic Acid Research vol. 29, (2001), no. 13, e62.
27. Prakash A. R., Amrein M. and Kaler K. V. I. S., "Characteristics and impact of Taq enzyme adsorption on surfaces in microfluidic devices", Microfluid Nanofluid vol. 4, (2008), 295-305.
28. Seidel C.A.M., Schulz A. and Sauer M.M.H., "Nucleobase-specific quenching of Fuorescent dyes. 1. Nucleobase one-electron redox potentials and their correlation with static and dynamic quenching efficiencies", J. Phys. Chem. Vol. 100, (1996), 5541-5553.
29. Crockett, A.O. and Wittwer, C.T., "Fluorescein-labeled oligonucleotides for real-time PCR: using the inherent quenching of deoxyguanosine nucleotides", Anal. Biochem. Vol. 290, (2001), 89-97.
30. Prakash R. and Kaler K.V.I.S., "DEP actuation of emulsion jets and dispensing of sub-nanoliter emulsion droplets", Lab Chip, Vol. 9, (2009), pp 2836-2844.