Novel O-palmitolylated beta-E1 subunit of pyruvate dehydrogenase is phosphorylated during ischemia/reperfusion injury

Proteome Science

Volumn 8, Issue , 2010, Pages

  Folmes, Clifford D L ^a   Sawicki, Grzegorz ^b,c   Cadete, Virgilio J J ^b   Masson, Grant ^a   Barr, Amy J ^a   Lopaschuk, Gary D ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b UNIVERSITY OF SASKATCHEWAN   (Canada)

^c WROCLAW MEDICAL UNIVERSITY   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; ATP SYNTHASE ALPHA SUBUNIT; ATP SYNTHASE D SUBUNIT; CYCLIC AMP DEPENDENT PROTEIN KINASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INSULIN RECEPTOR KINASE; LONG CHAIN ACYL COENZYME A DEHYDROGENASE; MITOCHONDRIAL CREATINE KINASE; PROTEIN KINASE C IOTA; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE BETA E1 SUBUNIT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; COMPUTER MODEL; CONTROLLED STUDY; ENZYME SUBUNIT; FATTY ACID OXIDATION; FUNCTIONAL PROTEOMICS; GLUCOSE OXIDATION; HEART FUNCTION; HEART MITOCHONDRION; HEART MUSCLE ISCHEMIA; ISOELECTRIC POINT; ISOLATED HEART; MALE; NONHUMAN; PALMITOYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; RAT; REPERFUSION INJURY; TIME OF FLIGHT MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

RATTUS;

EID: 77954317966     PISSN: None     EISSN: 14775956     Source Type: Journal    
DOI: 10.1186/1477-5956-8-38     Document Type: Article

References (52)

1. Fu Q, Van Eyk JE. Proteomics and heart disease: identifying biomarkers of clinical utility. Expert Rev Proteomics 2006, 3:237-249. 10.1586/14789450.3.2.237, 16608436.
2. Yuan C, Ravi R, Murphy AM. Discovery of disease-induced post-translational modifications in cardiac contractile proteins. Curr Opin Mol Ther 2005, 7:234-239.
3. Schwertz H, Langin T, Platsch H, Richert J, Bomm S, Schmidt M, Hillen H, Blaschke G, Meyer J, Darius H, Buerke M. Two-dimensional analysis of myocardial protein expression following myocardial ischemia and reperfusion in rabbits. Proteomics 2002, 2:988-995. 10.1002/1615-9861(200208)2:8<988::AID-PROT988>3.0.CO;2-#, 12203894.
4. White MY, Cordwell SJ, McCarron HC, Prasan AM, Craft G, Hambly BD, Jeremy RW. Proteomics of ischemia/reperfusion injury in rabbit myocardium reveals alterations to proteins of essential functional systems. Proteomics 2005, 5:1395-1410. 10.1002/pmic.200400995, 15800873.
5. White MY, Tchen AS, McCarron HC, Hambly BD, Jeremy RW, Cordwell SJ. Proteomics of ischemia and reperfusion injuries in rabbit myocardium with and without intervention by an oxygen-free radical scavenger. Proteomics 2006, 6:6221-6233. 10.1002/pmic.200600219, 17133370.
6. Arrell DK, Elliott ST, Kane LA, Guo Y, Ko YH, Pedersen PL, Robinson J, Murata M, Murphy AM, Marban E, Van Eyk JE. Proteomic analysis of pharmacological preconditioning: novel protein targets converge to mitochondrial metabolism pathways. Circ Res 2006, 99:706-714. 10.1161/01.RES.0000243995.74395.f8, 16946135.
7. Kim N, Lee Y, Kim H, Joo H, Youm JB, Park WS, Warda M, Cuong DV, Han J. Potential biomarkers for ischemic heart damage identified in mitochondrial proteins by comparative proteomics. Proteomics 2006, 6:1237-1249. 10.1002/pmic.200500291, 16402359.
8. Zhang J, Liem DA, Mueller M, Wang Y, Zong C, Deng N, Vondriska TM, Korge P, Drews O, Maclellan WR, et al. Altered proteome biology of cardiac mitochondria under stress conditions. J Proteome Res 2008, 7:2204-2214. 10.1021/pr070371f, 18484766.
9. Stanley WC, Recchia FA, Lopaschuk GD. Myocardial substrate metabolism in the normal and failing heart. Physiol Rev 2005, 85:1093-1129. 10.1152/physrev.00006.2004, 15987803.
10. Liedtke AJ, Demaison L, Eggleston AM, Cohen LM, Nellis SH. Changes in substrate metabolism and effects of excess fatty acids in reperfused myocardium. Circ Res 1988, 62:535-542.
11. Lopaschuk GD, Spafford MA, Davies NJ, Wall SR. Glucose and palmitate oxidation in isolated working rat hearts reperfused after a period of transient global ischemia. Circ Res 1990, 66:546-553.
12. Lerch R, Tamm C, Papageorgiou I, Benzi RH. Myocardial fatty acid oxidation during ischemia and reperfusion. Mol Cell Biochem 1992, 116:103-109. 10.1007/BF01270576, 1282666.
13. Lopaschuk GD, Collins-Nakai R, Olley PM, Montague TJ, Mcneil G, Gayle M, Penkoske P, Finegan BA. Plasma fatty acid levels in infants and adults after myocardial ischemia. Am Heart J 1994, 128:61-67. 10.1016/0002-8703(94)90010-8, 8017285.
14. Kantor PF, Dyck JR, Lopaschuk GD. Fatty acid oxidation in the reperfused ischemic heart. Am J Med Sci 1999, 318:3-14. 10.1097/00000441-199907000-00002, 10408755.
15. Opie LH. Myocardial ischemia--metabolic pathways and implications of increased glycolysis. Cardiovasc Drugs Ther 1990, 4(Suppl 4):777-790. 10.1007/BF00051275, 1965525.
16. Dennis SC, Gevers W, Opie LH. Protons in ischemia: where do they come from; where do they go to?. J Mol Cell Cardiol 1991, 23:1077-1086. 10.1016/0022-2828(91)91642-5, 1658348.
17. Hochachka PW, Mommsen TP. Protons and anaerobiosis. Science 1983, 219:1391-1397. 10.1126/science.6298937, 6298937.
18. Karmazyn M, Moffat MP. Na+/H+ exchange and regulation of intracellular Ca2+. 1993, 27:2079-2080.
19. Orchard CH, Houser SR, Kort AA, Bahinski A, Capogrossi MC, Lakatta EG. Acidosis facilitates spontaneous sarcoplasmic reticulum Ca2+ release in rat myocardium. J Gen Physiol 1987, 90:145-165. 10.1085/jgp.90.1.145, 2228866, 3625165.
20. Carmeliet E. Cardiac ionic currents and acute ischemia: from channels to arrhythmias. Physiol Rev 1999, 79:917-1017.
21. Liu B, Clanachan AS, Schulz R, Lopaschuk GD. Cardiac efficiency is improved after ischemia by altering both the source and fate of protons. Circ Res 1996, 79:940-948.
22. Taylor SW, Fahy E, Zhang B, Glenn GM, Warnock DE, Wiley S, Murphy AN, Gaucher SP, Capaldi RA, Gibson BW, Ghosh SS. Characterization of the human heart mitochondrial proteome. Nat Biotechnol 2003, 21:281-286. 10.1038/nbt793, 12592411.
23. Barr RL, Lopaschuk GD. Methodology for measuring in vitro/ex vivo cardiac energy metabolism. J Pharmacol Toxicol Methods 2000, 43:141-152. 10.1016/S1056-8719(00)00096-4, 11150742.
24. Idell-Wenger JA, Grotyohann LW, Neely JR. An improved method for isolation of mitochondria in high yields from normal ischemic, and autolyzed rat hearts. Anal Biochem 1982, 125:269-276. 10.1016/0003-2697(82)90006-9, 7181089.
25. Sawicki G, Dakour J, Morrish DW. Functional proteomics of neurokinin B in the placenta indicates a novel role in regulating cytotrophoblast antioxidant defences. Proteomics 2003, 3:2044-2051. 10.1002/pmic.200300537, 14625867.
26. Sawicki G, Jugdutt BI. Detection of regional changes in protein levels in the in vivo canine model of acute heart failure following ischemia-reperfusion injury: functional proteomics studies. Proteomics 2004, 4:2195-2202. 10.1002/pmic.200300746, 15221779.
27. Zhang X, Shi L, Shu S, Wang Y, Zhao K, Xu N, Liu S, Roepstorff P. An improved method of sample preparation on AnchorChip targets for MALDI-MS and MS/MS and its application in the liver proteome project. Proteomics 2007, 7:2340-2349. 10.1002/pmic.200600184, 17570520.
28. Pappin DJ, Hojrup P, Bleasby AJ. Rapid identification of proteins by peptide-mass fingerprinting. Curr Biol 1993, 3:327-332. 10.1016/0960-9822(93)90195-T, 15335725.
29. Bizzozero OA. Chemical analysis of acylation sites and species. Lipid modifications of proteins (Methods in enzymology Volume 250) 1995, 250:361-379. Academic Press, Casey PJ, Buss JE.
30. Quin J, Zhang X. Identification of in vivo protein phosphorylation sites with mass spectrometry. Posttranslational modifications of proteins Tools for functional proteomics 2002, 211. Totowa New Jersey Humana Press, Kannicht C.
31. Nelson DC, Cox MM. Lehninger Principles of Biochemistry. 2000, New York: Worth Publishers, 3.
32. Sugden MC, Holness MJ. Mechanisms underlying regulation of the expression and activities of the mammalian pyruvate dehydrogenase kinases. Arch Physiol Biochem 2006, 112:139-149. 10.1080/13813450600935263, 17132539.
33. Kobayashi K, Neely JR. Effects of ischemia and reperfusion on pyruvate dehydrogenase activity in isolated rat hearts. J MolCell Cardiol 1983, 15:359-367.
34. Churchill EN, Murriel CL, Chen CH, Mochly-Rosen D, Szweda LI. Reperfusion-induced translocation of deltaPKC to cardiac mitochondria prevents pyruvate dehydrogenase reactivation. Circ Res 2005, 97:78-85. 10.1161/01.RES.0000173896.32522.6e, 15961716.
35. Mayr M, Chung YL, Mayr U, McGregor E, Troy H, Baier G, Leitges M, Dunn MJ, Griffiths JR, Xu Q. Loss of PKC-delta alters cardiac metabolism. Am J Physiol Heart Circ Physiol 2004, 287:H937-945. 10.1152/ajpheart.00877.2003, 15277208.
36. Mayr M, Liem D, Zhang J, Li X, Avliyakulov NK, Yang JI, Young G, Vondriska TM, Ladroue C, Madhu B, et al. Proteomic and metabolomic analysis of cardioprotection: Interplay between protein kinase C epsilon and delta in regulating glucose metabolism of murine hearts. J Mol Cell Cardiol 2009, 46:268-277. 10.1016/j.yjmcc.2008.10.008, 19027023.
37. Mayr M, Metzler B, Chung YL, McGregor E, Mayr U, Troy H, Hu Y, Leitges M, Pachinger O, Griffiths JR, et al. Ischemic preconditioning exaggerates cardiac damage in PKC-delta null mice. Am J Physiol Heart Circ Physiol 2004, 287:H946-956. 10.1152/ajpheart.00878.2003, 15277209.
38. Collins-Nakai RL, Noseworthy D, Lopaschuk GD. Epinephrine increases ATP production in hearts by preferentially increasing glucose metabolism. Am J Physiol 1994, 267:1862-1871.
39. Depre C, Ponchaut S, Deprez J, Maisin L, Hue L. Cyclic AMP Suppresses the Inhibition of Glycolysis by Alternative Oxidizable Substrates in the Heart. Journal of Clinical Investigation 1998, 101:390-397. 10.1172/JCI1168, 508578, 9435311.
40. Folmes CD, Clanachan AS, Lopaschuk GD. Fatty acids attenuate insulin regulation of 5'-AMP-activated protein kinase and insulin cardioprotection after ischemia. Circ Res 2006, 99:61-68. 10.1161/01.RES.0000229656.05244.11, 16741157.
41. Roche TE, Hiromasa Y. Pyruvate dehydrogenase kinase regulatory mechanisms and inhibition in treating diabetes heart ischemia and cancer. Cell Mol Life Sci 2007, 64:830-849. 10.1007/s00018-007-6380-z, 17310282.
42. Lee FN, Zhang L, Zheng D, Choi WS, Youn JH. Insulin suppresses PDK-4 expression in skeletal muscle independently of plasma FFA. Am J Physiol Endocrinol Metab 2004, 287:E69-74. 10.1152/ajpendo.00461.2003, 15026305.
43. Branton WD, Rudnick MS, Zhou Y, Eccleston ED, Fields GB, Bowers LD. Fatty acylated toxin structure. Nature 1993, 365:496-497. 10.1038/365496a0, 8413602.
44. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999, 402:656-660. 10.1038/45230, 10604470.
45. Smotrys JE, Linder ME. Palmitoylation of intracellular signaling proteins: regulation and function. Annu Rev Biochem 2004, 73:559-587. 10.1146/annurev.biochem.73.011303.073954, 15189153.
46. Nestorescu ML, Siess EA, Wieland OH. Ultrastructural localization of pyruvate dehydrogenase in rat heart muscle. Histochemie 1973, 34:355-360. 10.1007/BF00306307, 4697786.
47. White MY, Edwards AVG, Cordwell SJ, Eyk JEV. Mitochondria: A mirror into cellular dysfunction in heart disease. Proteomics - Clinical Applications 2008, 2:845-861.
48. Di Lisa F, Menabo R, Canton M, Barile M, Bernardi P. Opening of the mitochondrial permeability transition pore causes depletion of mitochondrial and cytosolic NAD+ and is a causative event in the death of myocytes in postischemic reperfusion of the heart. J Biol Chem 2001, 276:2571-2575. 10.1074/jbc.M006825200, 11073947.
49. Fert-Bober J, Basran RS, Sawicka J, Sawicki G. Effect of duration of ischemia on myocardial proteome in ischemia/reperfusion injury. Proteomics 2008, 8:2543-2555. 10.1002/pmic.200800022, 18563752.
50. Williams EH, Kao RL, Morgan HE. Protein degradation and synthesis during recovery from myocardial ischemia. Am J Physiol 1981, 240:E268-273.
51. Xu Y, Kumar D, Dyck JR, Ford WR, Clanachan AS, Lopaschuk GD, Jugdutt BI. AT(1) and AT(2) receptor expression and blockade after acute ischemia-reperfusion in isolated working rat hearts. Am J Physiol Heart Circ Physiol 2002, 282:H1206-1215.
52. Mitch WE, Medina R, Grieber S, May RC, England BK, Price SR, Bailey JL, Goldberg AL. Metabolic acidosis stimulates muscle protein degradation by activating the adenosine triphosphate-dependent pathway involving ubiquitin and proteasomes. J Clin Invest 1994, 93:2127-2133. 10.1172/JCI117208, 294343, 8182144.