Enzymatic synthesis of 2′-deoxyadenosine and 6-methylpurine-2′-deoxyriboside by Escherichia coli DH5α overexpressing nucleoside phosphorylases from Escherichia coli BL21

Journal of Bioscience and Bioengineering

Volumn 110, Issue 2, 2010, Pages 165-168

  Liang, Shenghua ^a   Li, Wenzhou ^a   Gao, Tong ^a   Zhu, Xiangying ^a   Yang, Guimei ^a   Ren, Daming ^a  

^a FUDAN UNIVERSITY   (China)

Author keywords

2 Deoxyadenosine; 6 Methylpurine 2 deoxyriboside; Enzymatic synthesis; Escherichia coli; Nucleoside phosphorylases

Indexed keywords

CELL MASS; CELL PASTES; DEOXYADENOSINE; E. COLI; ENZYMATIC SYNTHESIS; EXPERIMENTAL PARAMETERS; GENES ENCODING; NUCLEOSIDE PHOSPHORYLASE; PURINE NUCLEOSIDE PHOSPHORYLASE; REAGENT CONCENTRATION; RECOMBINANT STRAINS;

BIOMOLECULES; ESCHERICHIA COLI; OPTIMIZATION; PHOSPHORYLATION;

ENZYME ACTIVITY;

6 METHYLPURINE; 6 METHYLPURINE 2' DEOXYRIBOSIDE; ADENINE; DEOXYADENOSINE; NUCLEOSIDE PHOSPHORYLASE; THYMIDINE PHOSPHORYLASE; UNCLASSIFIED DRUG; URIDINE PHOSPHORYLASE;

ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; BIOCATALYST; CONTROLLED STUDY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENETIC CODE; MOLECULAR CLONING; NONHUMAN; PH MEASUREMENT; TEMPERATURE SENSITIVITY;

DEOXYADENOSINES; ESCHERICHIA COLI; PENTOSYLTRANSFERASES; PROTEIN ENGINEERING; PURINE NUCLEOSIDES; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 77954309447     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2010.01.017     Document Type: Article

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