Transcuticular optical imaging of stimulus-evoked neural activities in the Drosophila peripheral nervous system

Nature Protocols

Volumn 5, Issue 7, 2010, Pages 1229-1235

  Kamikouchi, Azusa ^a   Wiek, Robert ^b   Effertz, Thomas ^b   Göpfert, Martin C ^b   Fiala, André ^b  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTENNA; ARTICLE; CALCIUM SIGNALING; CALCIUM TRANSPORT; CAMERA; CELL FUNCTION; CLINICAL PROTOCOL; DROSOPHILA; ELECTROPHYSIOLOGY; FEMORAL CHORDOTONAL ORGAN; FLUORESCENCE MICROSCOPY; IMAGE PROCESSING; IMAGING; JOHNSTON ORGAN; MECHANORECEPTOR; MULTIPHOTON MICROSCOPY; NONHUMAN; PERCEPTION; PERIPHERAL NERVOUS SYSTEM; PRIORITY JOURNAL; SENSORY NERVE CELL; STRETCH RECEPTOR; TECHNIQUE; TRANSCUTICULAR OPTICAL IMAGING; ANIMAL; AUDITORY STIMULATION; CYTOLOGY; DROSOPHILA MELANOGASTER; ELECTROSTIMULATION; GENETICS; MECHANOTRANSDUCTION; METABOLISM; PHYSIOLOGY; PROCEDURES; SIGNAL TRANSDUCTION; TOUCH; TRANSGENIC ANIMAL;

CALCIUM; CALCIUM BINDING PROTEIN; CAMELEON 2.1 PROTEIN, DROSOPHILA; DROSOPHILA PROTEIN;

ACOUSTIC STIMULATION; ANIMALS; ANIMALS, GENETICALLY MODIFIED; CALCIUM; CALCIUM-BINDING PROTEINS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ELECTRIC STIMULATION; MECHANOTRANSDUCTION, CELLULAR; MICROSCOPY, FLUORESCENCE; PERIPHERAL NERVOUS SYSTEM; SENSORY RECEPTOR CELLS; SIGNAL TRANSDUCTION; TOUCH;

EID: 77954261758     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.85     Document Type: Article

References (42)

1. Sokolowski, M.B. Drosophila: genetics meets behaviour. Nat. Rev. Genet. 2, 879-890 (2001).
2. Olsen, S.R. & Wilson, R.I. Cracking neural circuits in a tiny brain: new approaches for understanding the neural circuitry of Drosophila. Trends Neurosci. 31, 512-520 (2008).
3. Brand, A.H. & Perrimon, N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118, 401-415 (1998).
4. Duffy, J.B. GAL4 system in Drosophila: a fy geneticist's Swiss army knife. Genesis 34, 1-15 (2002).
5. McGuire, S.E., Roman, G. & Davis, R.L. Gene expression systems in Drosophila: a synthesis of time and space. Trends Genet. 20, 384-391 (2004).
6. Nitabach, M.N., Blau, J. & Holmes, T.C. Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock. Cell 109, 485-495 (2002).
7. Sweeney, S.T., Broadie, K., Keane, J., Niemann, H. & O'Kane, C.J. Targeted expression of tetanus toxin light chain in Drosophila specifcally eliminates synaptic transmission and causes behavioral defects. Neuron 14, 341-351 (1995).
8. Kitamoto, T. Targeted expression of temperature-sensitive dynamin to study neural mechanisms of complex behavior in Drosophila. J. Neurogenet. 16, 205-228 (2002).
9. Schroll, C. et al. Light-induced activation of distinct modulatory neurons triggers appetitive or aversive learning in Drosophila larvae. Curr. Biol. 16, 1741-1747 (2006).
10. Suh, G.S. et al. Light activation of an innate olfactory avoidance response in Drosophila. Curr. Biol. 17, 905-908 (2007).
11. Zhang, W., Ge, W. & Wang, Z. A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons. Eur. J. Neurosci. 26, 2405-2416 (2007).
12. Pulver, S.R., Pashkovski, S.L., Hornstein, N.J., Garrity, P.A. & Griffth, L.C. Temporal dynamics of neuronal activation by Channelrhodopsin-2 and TRPA1 determine behavioral output in Drosophila larvae. J. Neurophysiol. 101, 3075-3088 (2009).
13. Fiala, A. & Spall, T. In vivo calcium imaging of brain activity in Drosophila by transgenic cameleon expression. Sci. STKE 174, PL6 (2003).
14. Miyawaki, A. Fluorescence imaging of physiological activity in complex systems using GFP-based probes. Curr. Opin. Neurobiol. 13, 591-596 (2003).
15. Reiff, D.F. et al. In vivo performance of genetically encoded indicators of neural activity in fies. J. Neurosci. 25, 4766-4778 (2005).
16. Jayaraman, V. & Laurent, G. Evaluating a genetically encoded optical sensor of neural activity using electrophysiology in intact adult fruit fies. Front. Neural. Circuits 1, 3 (2007).
17. Hendel, T. et al. Fluorescence changes of genetic calcium indicators and OGB-1 correlated with neural activity and calcium in vivo and in vitro. J. Neurosci. 28, 7399-7411 (2008).
18. Vosshall, L.B. & Stocker, R.F. Molecular architecture of smell and taste in Drosophila. Annu. Rev. Neurosci. 30, 505-533 (2007).
19. Borst, A. Drosophila's view on insect vision. Curr. Biol. 19, 36-47 (2009).
20. Amrein, H. & Thorne, N. Gustatory perception and behavior in Drosophila melanogaster. Curr. Biol. 15, 673-684 (2005).
21. Kamikouchi, A. et al. The neural basis of Drosophila gravity-sensing and hearing. Nature 458, 165-171 (2009).
22. Yorozu, S. et al. Distinct sensory representations of wind and near-feld sound in the Drosophila brain. Nature 458, 201-205 (2009).
23. Fiala, A. Olfaction and olfactory learning in Drosophila: recent progress. Curr. Opin. Neurobiol. 17, 720-726 (2007).
24. Fiala, A. et al. Genetically expressed cameleon in Drosophila melanogaster is used to visualize olfactory information in projection neurons. Curr. Biol. 12, 1877-1884 (2002).
25. Wang, J.W., Wong, A.M., Flores, J., Vosshall, L.B. & Axel, R. Two-photon calcium imaging reveals an odor-evoked map of activity in the fy brain. Cell 112, 271-282 (2003).
26. Wang, Y. et al. Stereotyped odor-evoked activity in the mushroom body of Drosophila revealed by green fuorescent protein-based Ca2+ imaging. J. Neurosci. 24, 6507-6514 (2004).
27. Marella, S. et al. Imaging taste responses in the fy brain reveals a functional map of taste category and behavior. Neuron 49, 285-295 (2006).
28. 2 avoidance behaviour in Drosophila by inhibitory odorants. Nature 461, 277-281 (2009).
29. de Bruyne, M., Foster, K. & Carlson, J.R. Odor coding in the Drosophila antenna. Neuron 30, 537-1352 (2001).
30. Dobritsa, A.A., van der Goes van Naters, W., Warr, C.G., Steinbrecht, R.A. & Carlson, J.R. Integrating the molecular and cellular basis of odor coding in the Drosophila antenna. Neuron 37, 827-841 (2003).
31. Moon, S.J., Lee, Y., Jiao, Y. & Montell, C. A Drosophila gustatory receptor essential for aversive taste and inhibiting male-to-male courtship. Curr. Biol. 19, 1623-1627 (2009).
32. Albert, J.T., Nadrowski, B. & Göpfert, M.C. Mechanical signatures of transducer gating in the Drosophila ear. Curr. Biol. 17, 1000-1006 (2007).
33. Eberl, D.F., Hardy, R.W. & Kernan, M.J. Genetically similar transduction mechanisms for touch and hearing in Drosophila. J. Neurosci. 20, 5981-5988 (2000).
34. Miyawaki, A., Griesbeck, O., Heim, R. & Tsien, R.Y. Dynamic and quantitative Ca2+ measurements using improved cameleons. Proc. Natl Acad. Sci. USA 96, 2135-2140 (1999).
35. Diegelmann, S., Fiala, A., Leibold, C., Spall, T. & Buchner, E. Transgenic fies expressing the fuorescence calcium sensor Cameleon 2.1 under UAS control. Genesis 34, 95-98 (2002).
36. Pelz, D., Roeske, T., Syed, Z., de Bruyne, M. & Galizia, C.G. The molecular receptive range of an olfactory receptor in vivo (Drosophila melanogaster Or22a). J. Neurobiol. 66, 1544-1563 (2006).
37. Kamikouchi, A., Shimada, T. & Ito, K. Comprehensive classifcation of the auditory sensory projections in the brain of the fruit fy Drosophila melanogaster. J. Comp. Neurol. 499, 317-356 (2006).
38. Kim, J. et al. A TRPV family ion channel required for hearing in Drosophila. Nature 424, 81-84 (2003).
39. Sharma, Y., Cheung, U., Larsen, E.W. & Eberl, D.F. PPTGAL, a convenient Gal4 P-element vector for testing expression of enhancer fragments in Drosophila. Genesis 34, 115-118 (2002).
40. Albert, J.T., Nadrowski, B., Kamikouchi, A. & Göpfert, M.C. Mechanical tracing of protein function in the Drosophila ear. Nat. Protoc. [online] doi:10.1038/nprot.2006.364 (2006).
41. Nadrowski, B., Albert, J.T. & Göpfert, M.C. Transducer-based force generation explains active process in Drosophila hearing. Curr. Biol. 18, 1365-1372 (2008).
42. Göpfert, M.C. & Robert, D. The mechanical basis of Drosophila audition. J. Exp. Biol. 205, 1199-1208 (2002)