Molecular characterization of microbial communities in Canadian pulp and paper activated sludge and quantification of a novel Thiothrix eikelboomii-like bulking filament

Canadian Journal of Microbiology

Volumn 52, Issue 5, 2006, Pages 494-500

  Dumonceaux, Tim J ^a,d   Hill, Janet E ^a,b   Pelletier, Carl P ^c   Paice, Michael G ^c   Van Kessel, Andrew G ^a   Hemmingsen, Sean M ^b  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c PAPRICAN   (Canada)

^d Public Health Agency of Canada   (Canada)

Author keywords

021N; Activated sludge; Biological treatment; Bulking; chaperonin 60; Cpn60; Filamentous bacteria; Microbial communities; Mixed liquor suspended solids; qPCR; Settling; Thiothrix

Indexed keywords

ACTIVATED SLUDGE PROCESS; DATABASE SYSTEMS; DNA; FLUORESCENCE; GENES; MOLECULAR STRUCTURE; PAPER AND PULP INDUSTRY; REAL TIME SYSTEMS; WASTEWATER TREATMENT;

021N; ACTIVATED SLUDGE; BIOLOGICAL TREATMENT; BULKING; CHAPERONIN 60; CPN60; FILAMENTOUS BACTERIA; MICROBIAL COMMUNITIES; MIXED LIQUOR SUSPENDED SOLIDS (MLSS); QPCR; SETTLING;

MICROORGANISMS;

BACTERIAL DNA; CHAPERONIN; RIBOSOME DNA;

ACTIVATED SLUDGE; BACTERIUM; COMMUNITY STRUCTURE; DNA FINGERPRINTING; GENE; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION; REAL TIME; SUSPENDED LOAD;

ACTIVATED SLUDGE; ARTICLE; BACTERIAL STRAIN; CANADA; CONTROLLED STUDY; DATA BASE; DNA PROBE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE LIBRARY; GENE SEQUENCE; MICROFLORA; MICROSCOPY; NONHUMAN; PAPER INDUSTRY; PRIORITY JOURNAL; PULP MILL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SAMPLING; SUSPENDED PARTICULATE MATTER; TAXONOMY; THIOTHRIX; THIOTHRIX EIKELBOOMII; WASTE WATER MANAGEMENT;

ACTIVATED SLUDGE PROCESS; DATA BASES; GENES; MICROORGANISMS; MOLECULAR STRUCTURE; NUCLEIC ACIDS; PAPER INDUSTRY;

THIOTHRIX EIKELBOOMII;

EID: 33746606040     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W05-160     Document Type: Article

References (23)

1. Archibald, F., Methot, M., Young, F., and Paice, M.G. 2001. A simple system to rapidly monitor activated sludge health and performance. Water Res. 35(10): 2543-2553.
2. Bergeron, J., and Pelletier, C. 2004. Occurrence and significance of filamentous bacteria in pulp and paper activated sludge systems. Water Sci. Technol. 50(3): 39-48.
3. Brousseau, R., Hill, I.E., Prefontaine, G., Goh, S.-H., Harel, J., and Hemmingsen, S.M. 2001. Streptococcus suis serotypes characterized by analysis of chaperonin 60 gene sequences. Appl. Environ. Microbiol. 67(10): 4828-4833.
4. Dumonceaux, T.J., Hill, J.E., Briggs, S.A., Amoako, K.K., Hemmingsen, S.M., and Kessel, A.G.V. 2005. Enumeration of specific bacterial populations in complex intestinal communities using quantitative PCR based on the chaperonin 60 target. J. Microbiol. Meth. 64: 46-62.
5. Eikelboom, D. 2000. Process control of activated sludge plants by microscopic investigation. IWA Publishing, London.
6. Eikelboom, D.H., and Geurkink, B. 2002. Filamentous microorganisms observed in industrial activated sludge plants. Water Sci. Technol. 46(1-2): 535-542.
7. Fourest, E., Craperi, D., Deschamps-Roupert, C., Pisicchio, J.L., and Lenon, G. 2004. Occurrence and control of filamentous bulking in aerated wastewater treatment plants of the French paper industry. Water Sci. Technol. 50(3): 29-37.
8. Goh, S., Potter, S., Wood, J., Hemmingsen, S., Reynolds, R., and Chow, A. 1996. HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. J. Clin. Microbiol. 34(4): 818-823.
9. Hill, J.E., Seipp, R.P., Betts, M., Hawkins, L., Van Kessel, A.G., Crosby, W.L., and Hemmingsen, S.M. 2002. Extensive profiling of a complex microbial community by high-throughput sequencing. Appl. Environ. Microbiol. 68(6): 3055-3066.
10. Hill, J.E., Penny, S.L., Crowell, K.G., Goh, S.H., and Hemmingsen, S.M. 2004. cpnDB: a chaperonin sequence database. Genome Res. 14: 1669-1675.
11. Hill, J.E., Hemmingsen, S.M., Goldade, B.C., Dumonceaux, T.J., Klassen, J., Zijlstra, R.T., Goh, S.H., and Van Kessel, A.G. 2005. Comparison of ileum microflora of pigs fed corn-, wheat-, or barley-based diets by chaperonin-60 sequencing and quantitative PCR. Appl. Environ. Microbiol. 71(2): 867-875.
12. Hill, J.E., Town, J.R., and Hemmingsen, S.M. 2006. Improved template representation in cpn60 PCR product libraries generated from complex templates by application of a specific mixture of PCR primers. Environ. Microbiol. 8(4): 741-746.
13. Jenkins, D., and Richard, M. 2003. Manual on the causes and control of activated sludge bulking and foaming. IWA Publishing, London.
14. Jian, W., Zhu, L., and Dong, X. 2001. New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences. Int. J. Syst. Evol. Microbiol. 51: 1633-1638.
15. Kanagawa, T., Kamagata, Y., Aruga, S., Kohno, T., Horn, M., and Wagner, M. 2000. Phylogenetic analysis of and oligonucleotide probe development for eikelboom type 021N filamentous bacteria isolated from bulking activated sludge. Appl. Environ. Microbiol. 66(11): 5043-5052.
16. Liao, J., Lou, I., and de los Reyes, F.L., III. 2004. Relationship of species-specific filament levels to filamentous bulking in activated sludge. Appl. Environ. Microbiol. 70(4): 2420-2428.
17. Pearson, W.R. and Lipman, D.J. 1988. Improved tools for biological sequence comprison. Proc. Natl. Acad. Sci. U.S.A. 85: 2444-2448.
18. Richard, M. 1997. Recent changes in the prevalence and causes of bulking filamentous bacteria in pulp and paper mill activated sludge systems. In Proceedings of the TAPPI International Environmental Conference. Vol.2. Tappi Press, Norcross, Ga., USA. pp. 553-556.
19. Seviour, R.J., and Blackall, L.L. 1999. The microbiology of activated sludge. Dordrecht, Boston.
20. Smith, N.R., Yu, Z., and Mohn, W.W. 2003. Stability of the bacterial community in a pulp mill effluent treatment system during normal operation and a system shutdown. Water Res. 37(20): 4873-4884.
21. Thompson, G., Swain, J., Kay, M., and Forster, C.F. 2001. The treatment of pulp and paper mill effluent: a review. Bioresour. Technol. 77(3): 275-286.
22. Wilson, I. 1997. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63(10): 3741-3751.
23. Yu, Z., and Mohn, W.W. 1999. Killing two birds with one stone: simultaneous extraction of DNA and RNA from activated sludge biomass. Can. J. Microbiol. 45(3): 269-272.