Detection of Actinobacteria cultivated from environmental samples reveals bias in universal primers

Letters in Applied Microbiology

Volumn 45, Issue 4, 2007, Pages 376-381

  Farris, M H ^a   Olson, J B ^a  

^a UNIVERSITY OF ALABAMA   (United States)

Author keywords

16S rRNA gene; Actinobacteria; Oligotrophic media; Primer bias; Universal primers

Indexed keywords

BACTERIA; POLYMERASE CHAIN REACTION; RNA;

16S RRNA GENE; ACTINOBACTERIA; AMPLIFICATION PRODUCTS; BACTERIAL PRIMERS; ENVIRONMENTAL SAMPLE; OLIGOTROPHIC MEDIUM; PRIMER BIAS; SOIL EXTRACTS; SPECIFIC PRIMERS; UNIVERSAL PRIMER;

GENES;

NUCLEOTIDE; RIBOSOME RNA;

BACTERIA; CULTIVATION; GENES; RNA;

BACTERIUM; DETECTION METHOD; GENE; POLYMERASE CHAIN REACTION; RNA; TERRESTRIAL ENVIRONMENT;

ACTINOBACTERIA; ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; ENVIRONMENT; GENE AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SOIL;

ACTINOBACTERIA; ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS);

EID: 34848925453     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02198.x     Document Type: Article

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