Detection of Vibrio cholerae O1 in animal stools collected in rural areas of the Limpopo Province

Water SA

Volumn 36, Issue 2, 2010, Pages 167-171

  Keshav, V ^a   Potgieter, N ^b   Barnard, T G ^a  

^a UNIVERSITY OF JOHANNESBURG   (South Africa)

^b UNIVERSITY OF VENDA   (South Africa)

Author keywords

Animal faeces; Cholera toxin; PCR; Vibrio cholerae

Indexed keywords

DISEASES; GENES; MAMMALS; RNA; RURAL AREAS;

CAUSATIVE AGENTS; CHOLERA TOXIN; DETECTION RATES; FAECAL SAMPLES; SOUTH AFRICA; VIBRIO CHOLERAE; VIBRIO CHOLERAE O1; WATER SOURCE;

POLYMERASE CHAIN REACTION;

BACTERIUM; CATTLE; CHOLERA; DETECTION METHOD; DISEASE SPREAD; FECES; GENETIC ANALYSIS; GOAT; PIG; POLYMERASE CHAIN REACTION; POULTRY; RURAL AREA; TOXIN; WATER SUPPLY;

ANIMALIA; BACTERIA (MICROORGANISMS); CAPRA HIRCUS; COLUMBA; EQUUS ASINUS; GALLUS GALLUS; SUIDAE; VIBRIO CHOLERAE;

EID: 77954236352     PISSN: 03784738     EISSN: 18167950     Source Type: Journal    
DOI: 10.4314/wsa.v36i2.183724     Document Type: Article

References (21)

1. BOOM R, SOL CJA, SALIMANS MMM, JANSEN CL, WERTHEIMVAN DILLEN PME and VAN DER NOORDA J (1990) Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28 (3) 495-503.
2. BORODINA TA, LEHRACH H and SOLDATOV AV (2003) DNA purification on homemade silica spin-columns. Anal. Biochem. 321 135-137.
3. BRASHER CW, DEPAOLA A, JONES DD and BEJ AK (1998) Detection of microbial pathogens in shellfish with mutliplex PCR. Curr. Microbiol. 37 101-107.
4. CHAKRABORTY S, MUKHOPADHYAY AK, BHADRA RK, GHOSH AN, MITRA R, SHIMADA T, YAMASAK S, FARUQUE SH, TAKED Y, COLWELL RR and NAIR GB (2000) Virulence genes in environmental strains of Vibrio cholerae. Appl. Environ. Microbiol. 66 (9) 4022-4028.
5. CHEN Y, JOHNSON JA, PUSCH GD, MORRIS JG and STINE OC (2007) The genome of non-O1 Vibrio cholerae NRT36S demonstrate the presence of pathogenic mechanisms that are distinct from those of O1 Vibrio cholerae. Infect. Immun. 75 (5) 2645-2647.
6. COX P, GRIFFITH M, ANGLES M, DEERE D and FERGUSON C (2005) Concentrations of pathogens and indicators in animal faeces in the Sydney Watershed. Appl. Environ. Microbiol. 10 5929-5934.
7. FRAGA SG, PICHEL M, COSTAGLIOLA M, CECILIA M, JURQUIZA V, PERESUTI S, CAFFER MI, AULET O, HOZBOR C, TRACANNA BC, DE GAMUNDI AV, HERNANDEZ D and RAMIREZ FC (2007) Environment and virulence factors of Vibrio cholerae strains isolated in Argentina. J. Appl. Microbiol. 103 (6) 2448-2456.
8. GUNDRY SW, WRIGHT JA, CONROY R, DU PREEZ M, GENTHE B, MOYO S, MUTISI C, NDAMBA J and POTGIETER N (2006) Contamination of drinking water between source and point-of-use in rural households of South Africa and Zimbabwe: Implications for monitoring the millennium development goal for water. Water Pract. Technol. 1 1-9.
9. HUNTER PR (1997) Waterborne Disease: Epidemiology and Ecology. John Wiley and Sons. UK.
10. LIPP EK, RIVERA ING, GIL AI, ESPELAND EM, CHOOPUN N, LOUIS VR, RUSSEK-COHEN E, HUQ A and COLWELL RR (2003) Direct detection of Vibrio cholerae and ctxA in Peruvian coastal water and plankton by PCR. Appl. Environ. Microbiol. 69 3676-3680.
11. MIETA S (2009) Detection of Selected Entero-Pathogenic Bacteria from Stool Specimens Using a Novel Collection Technique. Master of Technology Dissertation, University of Johannesburg, South Africa.
12. MPAZI VM and MNYIKA KS (2005) Knowledge, attitudes and practices regarding cholera outbreaks in Ilala Municipality of Dar Es Salaam Region, Tanzania. East Afr. J. Public Health 2 6-11.
13. MUMY KL and FINDLAY RH (2004) Convenient determination of DNA extraction efficiency using an external DNA recovery standard and quantitative-competitive PCR. J. Microbiol. Methods 57 259-268.
14. NEVONDO TS and CLOETE TE (1999) Bacterial and chemical quality of water supply in the Dertig village settlement. Water SA 25 215-220.
15. NTEMA VM (2009) Detection of Vibrio cholerae and Vibrio parahaemolyticus by Molecular and Culture Methods from Source Water to Household Container-Stored Water at the Point-Of-Use in Rural Vhembe Communities in South Africa. Master of Technology Dissertation, University of Johannesburg, South Africa.
16. PERCIVAL SL, CHALMERS RM, EMBREY M, HUNTER PR, SELLWOOD J and WYN-JONES P (2004) Microbiology of Waterborne Diseases. Elsevier Academic Press. UK.
17. SANYAL SC, SINGH SJ, TIWARI IC, SEN PC, MARWAH SM, HAZARIKA UR, SINGH H, SHIMADA T and SAKAZAKI R (1974) Role of Household Animals in Maintenance of Cholera Infection in a Community. J. Infect. Dis. 130 (6) 575-579.
18. TARR CL, PATEL JS, PUHR ND, SOWERS EG, BOPP CA and STROCKBINE NA (2007) Identification of Vibrio isolates by amultiplex PCR assay and rpoB sequence determination. J. Clin. Microbiol. 45 (1) 145-140.
19. THERON J, CILLIERS J, DU PREEZ M, BROZEL VS and VENTER SN (2000) Detection of toxigenic Vibrio cholerae from environmental water samples by an enrichment broth cultivation-pit-stop semi-nested PCR procedure. J. Appl. Microbiol. 89 539-546.
20. THERON J and CLOETE TE (2004) Emerging microbiological detection techniques. In: In: Cloete TE, Rose J, Nel LH and Ford T (ed.). Microbial Waterborne Pathogens. IWA Publishing, UK.
21. VISSER IJ, VELLEMA P, VAN DOKKUM H and SHIMADA T (1999) Isolation of Vibrio cholerae from diseased farm animals and surface water in The Netherlands. Vet. Rec. 144 (16) 451-452.