Evidence of alternative splicing of the chi2 chitinase gene from Metarhizium anisopliae

Gene

Volumn 462, Issue 1-2, 2010, Pages 1-7

  Boldo, Juliano Tomazzoni ^a   do Amaral, Karina Bohrer ^a   Junges, Angela ^a   Pinto, Paulo Marcos ^a,b   Staats, Charley Christian ^a   Vainstein, Marilene Henning ^a   Schrank, Augusto ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b UNIVERSITY OF CAXIAS DO SUL   (Brazil)

Author keywords

Endochitinase chi2; Entomopathogenic fungi; Intron retention; Transcripts of chi2

Indexed keywords

CHITINASE; CHITINASE 2; UNCLASSIFIED DRUG;

ALTERNATIVE RNA SPLICING; ARTICLE; DNA SEQUENCE; GENE EXPRESSION; GENETIC TRANSCRIPTION; MASS SPECTROMETRY; METARHIZIUM ANISOPLIAE; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ARTHROPODA; FUNGI; METARHIZIUM ANISOPLIAE;

EID: 77954192318     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2010.04.005     Document Type: Article

References (35)

1. Abramoff M.D., Magalhaes P.J., Ram S.J. Image processing with ImageJ. Biophoton. Int. 2004, 11(7):36-42.
2. Adams D.J. Fungal cell wall chitinases and glucanases. Microbiol. 2004, 150(7):2029-2035.
3. Baba Y., Shimonaka A., Koga J., Kubota H., Kono T. Alternative splicing produces two endoglucanases with one or two carbohydrate-binding modules in Mucor circinelloides. J. Bacteriol. 2005, 187(9):3045-3051.
4. Baratto C.M., Dutra V., Boldo J.T., Leiria L.B., Vainstein M.H., Schrank A. Isolation, characterization, and transcriptional analysis of the chitinase chi2 gene (DQ011663) from the biocontrol fungus Metarhizium anisopliae var. anisopliae. Curr. Microbiol. 2006, 53(3):217-221.
5. Boel E., Hjort I., Svensson B., Norris F., Norris K.E., Fiil N.P. Glucoamylases G1 and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs. EMBO J. 1984, 3(5):1097-1102.
6. Boldo J.T., Junges A., Amaral K.B., Staats C.C., Vainstein M.H., Schrank A. Endochitinase CHI2 of the biocontrol fungus Metarhizium anisopliae affects its virulence toward the cotton stainer bug Dysdercus peruvianus. Curr Gen. 2009, 55(5):551-560.
7. Collins L., Penny D. Complex spliceosomal organization ancestral to extant eukaryotes. Mol. Biol. Evol. 2005, 22(4):1053-1066.
8. Dana M.M., Pintor-Toro J.A. Post-transcriptional control of a glucoamylase gene from Trichoderma harzianum under stress conditions. Mol. Microbiol. 2005, 57(1):250-260.
9. Davis C.A., Grate L., Spingola M., Ares M. Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast. Nucleic Acids Res. 2000, 28(8):1700-1706.
10. Schrank, A., Vainstein, M. H., (in press). Metarhizium anisopliae enzymes and toxins. Toxicon. doi: 10.1016/j.toxicon.2010.03.008.
11. Dutra V., Nakazato L., Broetto L., Silveira Schrank I., Vainstein M.H., Schrank A. Application of representational difference analysis to identify sequence tags expressed by Metarhizium anisopliae during the infection process of the tick Boophilus microplus cuticle. Res. Microbiol. 2004, 155(4):245-251.
12. Emri T., Molnar Z., Szilagyi M., Pocsi I. Regulation of autolysis in Aspergillus nidulans. Appl. Biochem. Biotechnol. 2008, 151(2-3):211-220.
13. Finn, R.D., Tate, J., Mistry, J., Coggill, P.C., Sammut, S.J., Hotz, H.R., Ceric, G., Forslund, K., Eddy, S.R., Sonnhammer, E.L., Bateman, A., 2008. The Pfam protein families database. Nucleic Acids Res. 36(Database issue), D281-288.
14. Frazzon A.P., da Silva Vaz Junior I., Masuda A., Schrank A., Vainstein M.H. In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus. Vet. Parasitol. 2000, 94(1-2):117-125.
15. Georg R.C., Stefani R.M.P., Gomes S.L. Environmental stresses inhibit splicing in the aquatic fungus Blastocladiella emersonii. BMC Microbiol. 2009, 9:231-241.
16. Gomez-Gomez E., Isabel M., Roncero G., Di Pietro A., Hera C. Molecular characterization of a novel endo-beta-1, 4-xylanase gene from the vascular wilt fungus Fusarium oxysporum. Curr. Genet. 2001, 40(4):268-275.
17. Hoppins S.C., et al. Alternative splicing gives rise to different isoforms of the Neurospora crassa Tob55 protein that vary in their ability to insert beta-barrel proteins into the outer mitochondrial membrane. Genet. 2007, 177(1):137-149.
18. Hunter S., et al. InterPro: the integrative protein signature database. Nucleic Acids Res. 2009, 37:D211-D215. Database issue.
19. Kupfer D.M., et al. Introns and splice elements of five diverse fungi. Eukaryot. Cell. 2004, 3(5):1088-1100.
20. Leal J., et al. A splice variant of the Neurospora crassa hex-1 transcript, which encodes the major protein of the Woronin body, is modulated by extracellular phosphate and pH changes. FEBS Lett. 2009, 583(1):180-184.
21. Lim L.P., Burge C.B. A computational analysis of sequence features involved in recognition of short introns. Proc. Natl. Acad. Sci. U.S.A. 2001, 98(20):11193-11198.
22. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 2001, 25(4):432-440.
23. Lubeck I., et al. Evaluation of Metarhizium anisopliae strains as potential biocontrol agents of the tick Rhipicephalus (Boophilus) microplus and the cotton stainer Dysdercus peruvianus. Fungal Ecol. 2008, 1(2-3):78-88.
24. Macarena S., Fernando L.L., Monica V., Rafael V., Bernardo G. Incomplete processing of peroxidase transcripts in the lignin degrading fungus Phanerochaete chrysosporium. FEMS Microbiol. Lett. 2005, 242(1):37-44.
25. McGuire A.M., Pearson M.D., Neafsey D.E., Galagan J.E. Cross-kingdom patterns of alternative splicing and splice recognition. Genome Biol. 2008, 9(3):R50.
26. Miller P.W., Dunn W.I., Schmidt R.R. Alternative splicing of a precursor-mRNA encoded by the Chlorella sorokiniana NADP-specific glutamate dehydrogenase gene yields mRNAs for precursor proteins of isozyme subunits with different ammonium affinities. Plant Mol. Biol. 1998, 37(2):243-263.
27. Pedrini N., Crespo R., Juárez M.P. Biochemistry of insect epicuticle degradation by entomopathogenic fungi. Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 2007, 146(1-2):124-137.
28. Roberts D.W., St Leger R.J. Metarhizium spp., cosmopolitan insect-pathogen fungi: mycological aspects. Adv. Appl. Microbiol. 2004, 54:1-70.
29. Sambrook J., Russel D. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 3rd ed.
30. Seidl V. Chitinases of filamentous fungi: a large group of diverse proteins with multiple physiological functions. Fungal Biol. Rev. 2008, 22(1):36-42.
31. Seidl V., Huemer B., Seiboth B., Kubicek C.P. A complete survey of Trichoderma chitinases reveals three distinct subgroups of family 18 chitinases. FEBS J. 2005, 272(22):5923-5939.
32. Sigrist C.J., et al. PROSITE: a documented database using patterns and profiles as motif descriptors. Brief. Bioinform. 2002, 3(3):265-274.
33. Wang K., Ussery D.W., Bunak S. Analysis and prediction of gene splice sites in four Aspergillus genomes. Fungal Genet. Biol. 2009, 46(Suppl 1):S14-S18.
34. Yamazaki H., Yamazaki D., Takaya N., Takagi M., Ohta A., Horiuchi H. A chitinase gene, chiB, involved in the autolytic process of Aspergillus nidulans. Curr. Genet. 2007, 51(2):89-98.
35. Ye D., Lee C.H., Queener S.F. Differential splicing of Pneumocystis carinii f. sp. carinii inosine 5'-monophosphate dehydrogenase pre-mRNA. Gene 2001, 263(1-2):151-158.