Isolation, characterization, and transcriptional analysis of the chitinase chi2 gene (DQ011663) from the biocontrol fungus Metarhizium anisopliae var. anisopliae

Current Microbiology

Volumn 53, Issue 3, 2006, Pages 217-221

  Baratto, César Milton ^a   Dutra, Valéria ^a   Boldo, Juliano Tomazzoni ^a   Leiria, Leonardo Barbosa ^a   Vainstein, Marilene Henning ^a   Schrank, Augusto ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; CHITINASE; GLUCOSE; GLYCOSIDASE; PROTEIN CRE1; PROTEIN CREA; PROTEIN CRR1; REPRESSOR PROTEIN; UNCLASSIFIED DRUG;

ARTHROPOD; ARTICLE; AUTOLYSIS; CHI2 GENE; COMPUTER PREDICTION; CUTICLE; DEGRADATION; GENE; GENE ISOLATION; GENETIC TRANSCRIPTION; HYDROLYSIS; INTRON; METARHIZIUM ANISOPLIAE; METARHIZIUM ANISOPLIAE VAR ANISOPLIAE; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN STRUCTURE;

BASE SEQUENCE; CHITINASE; DNA, FUNGAL; FUNGAL PROTEINS; HYPOCREALES; MOLECULAR SEQUENCE DATA; PEST CONTROL, BIOLOGICAL; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION INITIATION SITE;

ARTHROPODA; FUNGI; METARHIZIUM ANISOPLIAE; METARHIZIUM ANISOPLIAE VAR. ANISOPLIAE;

EID: 33747614096     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-006-0078-6     Document Type: Article

References (32)

1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402
2. Baratto CM, da Silva MV, Santi L, Passaglia L, Schrank IS, Vainstein MH, Schrank A (2003) Expression and characterization of the 42 kDa chitinase of the biocontrol fungus Metarhizium anisopliae in Escherichia coli. Can J Microbiol 49:723-726
3. Barreto CC, Staats CC, Schrank A, Vainstein MH (2004) Distribution of chitinases in the entomopathogen Metarhizium anisopliae and effect of N-acetylglucosamine in protein secretion. Curr Microbiol 48:102-107
4. Bogo MR, Queiroz MV, Silva DM, Gimenez Pecci MP, Azevedo JL, Schrank A (1996) Double-stranded RNA and isometric virus-like particles in the entomopathogenic fungus Metarhizium anisopliae. Mycol Res 100:1468-1472
5. Bogo MR, Rota CA, Pinto H, Jr., Ocampos M, Correa CT, Vainstein MH, Schrank A (1998) A chitinase encoding gene (chit1 gene) from the entomopathogen Metarhizium anisopliae: isolation and characterization of genomic and full-length cDNA. Curr Microbiol 37:221-225
6. Carsolio C, Gutierrez A, Jimenez B, Van MM, Herrera-Estrella A (1994) Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism. Proc Natl Acad Sci USA 91:10903-10907
7. Cubero B, Scazzocchio C (1994) Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J 13:407-415
8. da Silva MV, Santi L, Staats CC, da Costa AM, Colodel EM, Driemeier D, Vainstein MH, Schrank A (2005) Cuticle-induced endo/exoacting chitinase CHIT30 from Metarhizium anisopliae is encoded by an ortholog of the chi3 gene. Res Microbiol 156:382-392
9. Dutra V, Nakazato L, Broetto L, Silveira SI, Henning VM, Schrank A (2004) Application of representational difference analysis to identify sequence tags expressed by Metarhizium anisopliae during the infection process of the tick Boophilus microplus cuticle. Res Microbiol 155:245-251
10. Eberle J, Russo VE (1994) Neurospora crassa blue light-inducible gene bli-3. Biochem Mol Biol Int 34:737-744
11. Falquet L, Pagni M, Bucher P, Hulo N, Sigrist CJ, Hofmann K, Bairoch A (2002) The PROSITE database, its status in 2002. Nucleic Acids Res 30:235-238
12. Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Feng J, Yang X, Zhang Y, Pei Y (2005) Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol 71:(1)363-370
13. Frazzon AP, da Silva VJI, Masuda A, Schrank A, Vainstein MH (2000) In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus. Vet Parasitol 94:117-125
14. Gillespie JP, Bateman R, Charnley AK (1998) Role of cuticle-degrading proteases in the virulence of Metarhizium spp. for the desert locust, Schistocerca gregaria. J Invertebr Pathol 71:128-137
15. Kang SC, Park S, Lee DG (1999) Purification and characterization of a novel chitinase from the entomopathogenic fungus, Metarhizium anisopliae. J Invertebr Pathol 73:276-281
16. Kanzok SM, Jacobs-Lorena M (2006) Entomopathogenic fungi as biological insecticides to control malaria. Trends Parasitol 22:49-51
17. Kim DJ, Baek JM, Uribe P, Kenerley CM, Cook DR (2002) Cloning and characterization of multiple glycosyl hydrolase genes from Trichoderma virens. Curr Genet 40:374-384
18. Krieger de MC, Schrank A, Vainstein MH (2003) Regulation of extracellular chitinases and proteases in the entomopathogen and acaricide Metarhizium anisopliae. Curr Microbiol 46:205-210
19. Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244-1245
20. Limon MC, Chacon MR, Mejias R, gado-Jarana J, Rincon AM, Codon AC, Benitez T (2004) Increased antifungal and chitinase specific activities of Trichoderma harzianum CECT 2413 by addition of a cellulose binding domain. Appl Microbiol Biotechnol 64:675-685
21. Nakazato L, Dutra V, Broetto L, Staats CC, Vainstein MH, Schrank A (2006) Development of an expression vector for Metarhizium anisopliae based on the tef-1alpha homologous promoter. Appl Microbiol Biotechnol 10:1-8
22. Orikoshi H, Baba N, Nakayama S, Kashu H, Miyamoto K, Yasuda M, Inamori Y, Tsujibo H (2003) Molecular analysis of the gene encoding a novel cold-adapted chitinase (ChiB) from a marine bacterium, Alteromonas sp. strain O-7. J Bacteriol 185:1153-1160
23. Pinto AS, Barreto CC, Schrank A, Ulhoa CJ, Vainstein MH (1997) Purification and characterization of an extracellular chitinase from the entomopathogenic Metarhizium anisopliae. Can J Microbiol 43:322-327
24. Sambrook J, Russel DW (2001) Molecular cloning: A laboratory manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory
25. Screen S, Bailey A, Charnley K, Cooper R, Clarkson J (1997) Carbon regulation of the cuticle-degrading enzyme PR1 from Metarhizium anisopliae may involve a trans-acting DNA-binding protein CRR1, a functional equivalent of the Aspergillus nidulans CREA protein. Curr Genet 31:511-518
26. Screen SE, Hu G, St. Leger RJ (2001) Transformants of Metarhizium anisopliae sf. anisopliae overexpressing chitinase from Metarhizium anisopliae sf. acridum show early induction of native chitinase but are not altered in pathogenicity to Manduca sexta. J Invert Pathol 78:260-266
27. Seidl V, Huemer B, Seiboth B, Kubicek CP (2005) A complete survey of Trichoderma chitinases reveals three distinct subgroups of family 18 chitinases. FEBS J 272:5923-5939
28. St. Leger RJ, Charnley AK, Cooper RM (1986) Cuticle-degrading enzymes of entomopathogenic fungi: Mechanisms of interaction between pathogen enzymes and insect cuticle. J Invert Pathol 47:295-302
29. St. Leger RJ, Cooper RM, Charnley AK (1991) Characterization of chitinase and chitobiase produced by the entomopathogenic fungus Metarhizium anisopliae. J Invert Pathol 58:415-426
30. Takaya N, Yamazaki D, Horiuchi H, Ohta A, Takagi M (1998) Cloning and characterization of a chitinase-encoding gene (chiA) from Aspergillus nidulans, disruption of which decreases germination frequency and hyphal growth. Biosci Biotechnol Biochem 62:60-65
31. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876-4882
32. Viterbo A, Haran S, Friesem D, Ramot O, Chet I (2001) Antifungal activity of a novel endochitinase gene (chit36) from Trichoderma harzianum Rifai TM. FEMS Microbiol Lett 200:169-174