Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point

BMC Cell Biology

Volumn 11, Issue , 2010, Pages

  Frisa, Phyllis S ^a   Jacobberger, James W ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; ANTIBODY; APHIDICOLIN; CYCLINE; DNA; METHANOL; MIMOSINE; MINICHROMOSOME MAINTENANCE PROTEIN 6; NOCODAZOLE; TRITON X 100; ANTINEOPLASTIC AGENT; CELL CYCLE PROTEIN; MCM6 PROTEIN, HUMAN;

ARTICLE; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE S PHASE; CHROMATIN; CONTROLLED STUDY; CYTOMETRY; DNA CONTENT; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; LASER SCANNING CYTOMETRY; LYMPHOMA CELL LINE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; WESTERN BLOTTING; CELL DIFFERENTIATION; CELL SEPARATION; CULTURE MEDIUM; DRUG EFFECT; GENETICS; METABOLISM; PATHOLOGY; PROTEIN BINDING; T CELL LYMPHOMA; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS; APHIDICOLIN; CELL CYCLE PROTEINS; CELL DIFFERENTIATION; CELL LINE, TUMOR; CELL SEPARATION; CHROMATIN; CULTURE MEDIA, SERUM-FREE; DNA; FLOW CYTOMETRY; G1 PHASE; HUMANS; LYMPHOMA, T-CELL; MIMOSINE; NOCODAZOLE; PROLIFERATING CELL NUCLEAR ANTIGEN; PROTEIN BINDING;

EID: 77954164500     PISSN: None     EISSN: 14712121     Source Type: Journal    
DOI: 10.1186/1471-2121-11-26     Document Type: Article

References (43)

1. Hengstschlager M, Braun K, Soucek T, Miloloza A, Hengstschlager-Ottnad E. Cyclin-dependent kinases at the G1-S transition of the mammalian cell cycle. Mutation research 1999, 436(1):1-9. 10.1016/S1383-5742(98)00022-2, 9878675.
2. Roussel MF. Key effectors of signal transduction and G1 progression. Advances in cancer research 1998, 74:1-24. full_text, 9561265.
3. Arellano M, Moreno S. Regulation of CDK/cyclin complexes during the cell cycle. The international journal of biochemistry & cell biology 1997, 29(4):559-573.
4. Pines J. Mitosis: a matter of getting rid of the right protein at the right time. Trends in cell biology 2006, 16(1):55-63. 10.1016/j.tcb.2005.11.006, 16337124.
5. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry 1996, 25(1):1-13. 10.1002/(SICI)1097-0320(19960901)25:1<1::AID-CYTO1>3.0.CO;2-N, 8875049.
6. Juan G, Li X, Darzynkiewicz Z. Correlation between DNA replication and expression of cyclins A and B1 in individual MOLT-4 cells. Cancer research 1997, 57(5):803-807.
7. Juan G, Li X, Darzynkiewicz Z. Phosphorylation of retinoblastoma protein assayed in individual HL-60 cells during their proliferation and differentiation. Experimental cell research 1998, 244(1):83-92. 10.1006/excr.1998.4165, 9770352.
8. Jacobberger JW, Frisa PS, Sramkoski RM, Stefan T, Shults KE, Soni DV. A new biomarker for mitotic cells. Cytometry A 2008, 73(1):5-15.
9. Yen A, Pardee AB. Exponential 3T3 cells escape in mid-G1 from their high serum requirement. Experimental cell research 1978, 116(1):103-113. 10.1016/0014-4827(78)90068-X, 359338.
10. Yung Y, Walker JL, Roberts JM, Assoian RK. A Skp2 autoinduction loop and restriction point control. The Journal of cell biology 2007, 178(5):741-747. 10.1083/jcb.200703034, 2064539, 17724117.
11. Dimova DK, Dyson NJ. The E2F transcriptional network: old acquaintances with new faces. Oncogene 2005, 24(17):2810-2826. 10.1038/sj.onc.1208612, 15838517.
12. Sherr CJ. The Pezcoller lecture: cancer cell cycles revisited. Cancer research 2000, 60(14):3689-3695.
13. Planas-Silva MD, Weinberg RA. The restriction point and control of cell proliferation. Current opinion in cell biology 1997, 9(6):768-772. 10.1016/S0955-0674(97)80076-2, 9425340.
14. Zetterberg A, Larsson O, Wiman KG. What is the restriction point?. Current opinion in cell biology 1995, 7(6):835-842. 10.1016/0955-0674(95)80067-0, 8608014.
15. Novak B, Tyson JJ. A model for restriction point control of the mammalian cell cycle. J Theor Biol 2004, 230(4):563-579. 10.1016/j.jtbi.2004.04.039, 15363676.
16. Martinsson HS, Starborg M, Erlandsson F, Zetterberg A. Single cell analysis of G1 check points-the relationship between the restriction point and phosphorylation of pRb. Experimental cell research 2005, 305(2):383-391. 10.1016/j.yexcr.2005.01.023, 15817163.
17. Friedrich TD, Bedner E, Darzynkiewicz Z, Lehman JM. Distinct patterns of MCM protein binding in nuclei of S phase and rereplicating SV40-infected monkey kidney cells. Cytometry A 2005, 68(1):10-18.
18. Mukherjee P, Cao TV, Winter SL, Alexandrow MG. Mammalian MCM loading in late-G(1) coincides with Rb hyperphosphorylation and the transition to post-transcriptional control of progression into S-phase. PloS one 2009, 4(5):e5462. 10.1371/journal.pone.0005462, 2674209, 19421323.
19. Kurki P, Ogata K, Tan EM. Monoclonal antibodies to proliferating cell nuclear antigen (PCNA)/cyclin as probes for proliferating cells by immunofluorescence microscopy and flow cytometry. Journal of immunological methods 1988, 109(1):49-59. 10.1016/0022-1759(88)90441-3, 2895795.
20. Stillman B. Initiation of DNA replication. The Cell Division Cycle: Controlling when and where cells divide and differentiate 2009, The Biomedical & Life Sciences Collection, London: Henry Stewart Talks Ltd, Millar J., http://hstalks.com/bio
21. Blow J. Replication licensing. The Cell Division Cycle: Controlling when and where cells divide and differentiate 2009, The Biomedical & Life Sciences Collection, London: Henry Stewart Talks Ltd, Millar J., http://hstalks.com/bio
22. Sasaki T, Ramanathan S, Okuno Y, Kumagai C, Shaikh SS, Gilbert DM. The Chinese hamster dihydrofolate reductase replication origin decision point follows activation of transcription and suppresses initiation of replication within transcription units. Molecular and cellular biology 2006, 26(3):1051-1062. 10.1128/MCB.26.3.1051-1062.2006, 1347040, 16428457.
23. Chuang LC, Teixeira LK, Wohlschlegel JA, Henze M, Yates JR, Mendez J, Reed SI. Phosphorylation of Mcm2 by Cdc7 promotes pre-replication complex assembly during cell-cycle re-entry. Molecular cell 2009, 35(2):206-216. 10.1016/j.molcel.2009.06.014, 19647517.
24. DePamphilis ML, Blow JJ, Ghosh S, Saha T, Noguchi K, Vassilev A. Regulating the licensing of DNA replication origins in metazoa. Current opinion in cell biology 2006, 18(3):231-239. 10.1016/j.ceb.2006.04.001, 16650748.
25. Maiorano D, Lutzmann M, Mechali M. MCM proteins and DNA replication. Current opinion in cell biology 2006, 18(2):130-136. 10.1016/j.ceb.2006.02.006, 16495042.
26. Kearsey SE, Labib K. MCM proteins: evolution, properties, and role in DNA replication. Biochimica et biophysica acta 1998, 1398(2):113-136.
27. Takeda DY, Dutta A. DNA replication and progression through S phase. Oncogene 2005, 24(17):2827-2843. 10.1038/sj.onc.1208616, 15838518.
28. Prasanth SG, Mendez J, Prasanth KV, Stillman B. Dynamics of pre-replication complex proteins during the cell division cycle. Philosophical transactions of the Royal Society of London 2004, 359(1441):7-16. 10.1098/rstb.2003.1360, 1693299, 15065651.
29. Lohr F, Wenz F, Haas S, Flentje M. Comparison of proliferating cell nuclear antigen (PCNA) staining and BrdUrd-labelling index under different proliferative conditions in vitro by flow cytometry. Cell proliferation 1995, 28(2):93-104. 10.1111/j.1365-2184.1995.tb00058.x, 7893842.
30. Landberg G, Roos G. Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis. Cancer research 1991, 51(17):4570-4574.
31. Landberg G, Roos G. Expression of proliferating cell nuclear antigen (PCNA) and Ki-67 antigen in human malignant hematopoietic cells. Acta Oncol 1991, 30(8):917-921. 10.3109/02841869109088244, 1685657.
32. Korcz A, Soprano DR, Soprano KJ. Tyrosine protein kinase expression in long-term quiescent WI-38 cells following growth factor simulation. J Cell Biochem 1995, 59(1):42-52. 10.1002/jcb.240590106, 8530535.
33. Durcan TM, Halpin ES, Casaletti L, Vaughan KT, Pierson MR, Woods S, Hinchcliffe EH. Centrosome duplication proceeds during mimosine-induced G1 cell cycle arrest. J Cell Physiol 2008, 215(1):182-191. 10.1002/jcp.21298, 2764247, 17960592.
34. Dimitrova DS, Todorov IT, Melendy T, Gilbert DM. Mcm2, but not RPA, is a component of the mammalian early G1-phase prereplication complex. The Journal of cell biology 1999, 146(4):709-722. 10.1083/jcb.146.4.709, 2156148, 10459007.
35. Schepers A, Papior P. Why are we where we are? Understanding replication origins and initiation sites in eukaryotes using ChIP-approaches. Chromosome Res 2010, 18(1):63-77. 10.1007/s10577-009-9087-1, 19904620.
36. Ge XQ, Jackson DA, Blow JJ. Dormant origins licensed by excess Mcm2-7 are required for human cells to survive replicative stress. Genes & development 2007, 21(24):3331-3341.
37. Ibarra A, Schwob E, Mendez J. Excess MCM proteins protect human cells from replicative stress by licensing backup origins of replication. Proceedings of the National Academy of Sciences of the United States of America 2008, 105(26):8956-8961. 10.1073/pnas.0803978105, 2449346, 18579778.
38. Cadoret JC, Meisch F, Hassan-Zadeh V, Luyten I, Guillet C, Duret L, Quesneville H, Prioleau MN. Genome-wide studies highlight indirect links between human replication origins and gene regulation. Proceedings of the National Academy of Sciences of the United States of America 2008, 105(41):15837-15842. 10.1073/pnas.0805208105, 2572913, 18838675.
39. Wu PY, Nurse P. Establishing the program of origin firing during S phase in fission Yeast. Cell 2009, 136(5):852-864. 10.1016/j.cell.2009.01.017, 2787407, 19269364.
40. Desprat R, Thierry-Mieg D, Lailler N, Lajugie J, Schildkraut C, Thierry-Mieg J, Bouhassira EE. Predictable dynamic program of timing of DNA replication in human cells. Genome research 2009, 19(12):2288-2299. 10.1101/gr.094060.109, 19767418.
41. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227(5259):680-685. 10.1038/227680a0, 5432063.
42. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 1979, 76(9):4350-4354. 10.1073/pnas.76.9.4350, 411572, 388439.
43. Bagwell CB. Hyperlog-a flexible log-like transform for negative, zero, and positive valued data. Cytometry A 2005, 64(1):34-42.