Development of homogeneous luminescence assays for histone demethylase catalysis and binding

Analytical Biochemistry

Volumn 404, Issue 1, 2010, Pages 86-93

  Kawamura, Akane ^a   Tumber, Anthony ^b   Rose, Nathan R ^a   King, Oliver N F ^a   Daniel, Michelle ^b,c   Oppermann, Udo ^b,c   Heightman, Tom D ^b   Schofield, Christopher ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c NIHR OXFORD BIOMEDICAL RESEARCH CENTRE   (United Kingdom)

Author keywords

AlphaScreen; Epigenetics; Fe(II) and 2 oxoglutarate (2OG) oxygenases; Histone demethylase (HDM); JMJD2 KDM4

Indexed keywords

AMINO ACIDS; BINDING ENERGY; CATALYST ACTIVITY; GENE EXPRESSION; IRON COMPOUNDS; LUMINESCENCE; MOLECULES;

2-OXOGLUTARATE; ALPHASCREEN; DEMETHYLASE; EPIGENETICS; JMJD2/KDM4;

PROTEINS;

HISTONE; HISTONE DEMETHYLASE; UNCLASSIFIED DRUG; ENZYME INHIBITOR; ISOPROTEIN; JMJD2E PROTEIN, HUMAN; PEPTIDE; PROTEIN BINDING;

AMPLIFIED LUMINESCENT PROXIMITY HOMOGENOUS ASSAY; ARTICLE; ASSAY; BINDING AFFINITY; BIOLUMINESCENCE; BIOTINYLATION; CATALYSIS; EPIGENETICS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN METABOLISM; PROTEIN SYNTHESIS; BIOCATALYSIS; CHEMISTRY; HUMAN; LUMINESCENCE; METABOLISM; PROCEDURES; PROTEIN TERTIARY STRUCTURE;

BIOCATALYSIS; ENZYME INHIBITORS; HUMANS; JUMONJI DOMAIN-CONTAINING HISTONE DEMETHYLASES; LUMINESCENT MEASUREMENTS; PEPTIDES; PROTEIN BINDING; PROTEIN ISOFORMS; PROTEIN STRUCTURE, TERTIARY;

EID: 77954083147     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.04.030     Document Type: Article

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