Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses

Science

Volumn 328, Issue 5986, 2010, Pages 1705-1709

  Hapfelmeier, Siegfried ^a,b   Lawson, Melissa A E ^b   Slack, Emma ^a,b   Kirundi, Jorum K ^a,b   Stoel, Maaike ^b   Heikenwalder, Mathias ^c   Cahenzli, Julia ^b   Velykoredko, Yuliya ^b   Balmer, Maria L ^a   Endt, Kathrin ^d   Geuking, Markus B ^b   Curtiss III, Roy ^e   McCoy, Kathy D ^b   Macpherson, Andrew J ^a,b  

^a UNIVERSITY OF BERN   (Switzerland)

^b MCMASTER UNIVERSITY   (Canada)

^c UNIVERSITY HOSPITAL ZURICH   (Switzerland)

^d ETH ZURICH   (Switzerland)

^e ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; ANTIBODY; IMMUNOGLOBULIN A;

ANTIBIOTICS; ANTIBODY; BACTERIUM; DIET; HOST-PARASITE INTERACTION; IMMUNE RESPONSE; MICROBIAL ACTIVITY; MUTUALISM; PROTEIN; RODENT; STOMACH CONTENT; VACCINATION;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL COLONIZATION; COMMENSALISM; CONTROLLED STUDY; DIET; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTION; GERMFREE ANIMAL; IMMUNE RESPONSE; MOUSE; NONHUMAN; PRIORITY JOURNAL; VACCINATION;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIBODY SPECIFICITY; COLONY COUNT, MICROBIAL; DOSE-RESPONSE RELATIONSHIP, IMMUNOLOGIC; ESCHERICHIA COLI; GERM-FREE LIFE; HALF-LIFE; IMMUNOGLOBULIN A; IMMUNOLOGIC MEMORY; INTESTINAL MUCOSA; INTESTINES; MICE; MICE, INBRED C57BL; MUCOUS MEMBRANE; PLASMA CELLS; TIME FACTORS;

BACTERIA (MICROORGANISMS); MAMMALIA; MUS;

EID: 77954051526     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1188454     Document Type: Article

References (18)

1. A. J. Macpherson, T. Uhr, Science 303, 1662 (2004).
2. A. J. Macpherson et al., Science 288, 2222 (2000).
3. D. A. Peterson, N. P. McNulty, J. L. Guruge, J. I. Gordon, Cell Host Microbe 2, 328 (2007).
4. K. Nakayama, S. M. Kelly, R. Curtiss, Nat. Biotechnol. 6, 693 (1988).
5. K. E. Shroff, K. Meslin, J. J. Cebra, Infect. Immun. 63, 3904 (1995).
6. E. Slack et al., Science 325, 617 (2009).
7. Materials and methods are available as supporting material on Science Online.
8. B. Stecher et al., PLoS Pathog. 6, e1000711 (2010).
9. A. T. Bentley, P. E. Klebba, J. Bacteriol. 170, 1063 (1988).
10. R. M. Zinkernagel, H. Hengartner, Immunol. Rev. 211, 310 (2006).
11. D. F. Tough, Trends Immunol. 24, 404 (2003).
12. A. I. Fecsik, W. T. Butler, A. H. Coons, J. Exp. Med. 120, 1041 (1964).
13. N. L. Bernasconi, E. Traggiai, A. Lanzavecchia, Science 298, 2199 (2002).
14. C. A. Mattioli, T. B. Tomasi Jr., J. Exp. Med. 138, 452 (1973).
15. R. A. Manz, A. Thiel, A. Radbruch, Nature 388, 133 (1997).
16. M. K. Slifka, R. Antia, J. K. Whitmire, R. Ahmed, Immunity 8, 363 (1998).
17. Y. Cong, T. Feng, K. Fujihashi, T. R. Schoeb, C. O. Elson, Proc. Natl. Acad. Sci. USA. 106, 19256 (2009).
18. We thank D. Zhang, H. Roberts, J. Notarangelo, S. Armstrong, S. Competente, M. Provenza, J. Bodewes, and R. McArthur for their technical support and B. Stecher, W.-D. Hardt, A. J. Müller, N. A. Bos, C. Mueller, R. M. Zinkernagel, A. G. Rolink, A. Lanzavecchia, and C. Reis e Sousa for their helpful comments and editing the manuscript. Grant support: Swiss National Science Foundation (310030-124732), Canadian Institutes of Health Research, Crohn's and Colitis Foundation of Canada, Genome Canada, Farncombe Foundation, Canadian Association of Gastroenterology. This research was also supported with funding from the Canada Research Chairs program to K.D.M. and A.J.M. M.H. is a fellow of the Prof. Dr. Max Cloëtta foundation and was supported by grants of the Oncosuisse Foundation (OCS 02113-08-2007). S.H. was supported in part by a German Science Foundation fellowship, and the clean mouse facility Bern was supported by the Genaxen Foundation. The authors have no conflicts of interests to declare.