Activin signaling in microsatellite stable colon cancers is disrupted by a combination of genetic and epigenetic mechanisms

PLoS ONE

Volumn 4, Issue 12, 2009, Pages

  Jung, Barbara ^a,b,c   Gomez, Jessica ^b   Chau, Eddy ^a   Cabral, Jennifer ^a   Lee, Jeffrey K ^a   Anselm, Aimee ^a   Slowik, Przemyslaw ^a   Ream Robinson, Deena ^a   Messer, Karen ^b   Sporn, Judith ^a   Shin, Sung K ^d   Richard Boland, C ^d   Goel, Ajay ^d   Carethers, John M ^a,b,c  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^c VA SAN DIEGO HEALTHCARE SYSTEM   (United States)

^d BAYLOR UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; ACTIVIN RECEPTOR 1; ACTIVIN RECEPTOR 2; SMAD2 PROTEIN;

ADULT; ARTICLE; CANCER CELL CULTURE; CHROMOSOMAL INSTABILITY; COLON CANCER; CONTROLLED STUDY; DNA METHYLATION; EPIGENETICS; FEMALE; FLUORESCENCE ANALYSIS; GENE EXPRESSION; GENE LOSS; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC ASSOCIATION; HETEROZYGOSITY LOSS; HUMAN; HUMAN CELL; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; MICROSATELLITE MARKER; MUTATIONAL ANALYSIS; PHENOTYPE; POLYMERASE CHAIN REACTION; POPULATION RESEARCH; PRIMARY TUMOR; PROMOTER REGION; QUANTITATIVE STUDY; SIGNAL TRANSDUCTION; SOMATIC MUTATION; WESTERN BLOTTING;

ACTIVIN RECEPTORS, TYPE I; ACTIVIN RECEPTORS, TYPE II; CELL LINE, TUMOR; COLONIC NEOPLASMS; DNA METHYLATION; EPIGENESIS, GENETIC; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; LOSS OF HETEROZYGOSITY; MICROSATELLITE INSTABILITY; MICROSATELLITE REPEATS; MUTATION; PROMOTER REGIONS, GENETIC; SIGNAL TRANSDUCTION; SMAD2 PROTEIN; TRANSCRIPTION, GENETIC; TUMOR BURDEN;

EID: 77954043818     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0008308     Document Type: Article

References (25)

1. Carethers JM (2003) Biology of Colorectal Carcinoma., editors. In: Rustgi A, Crawford J, eds. Gastrointestinal Cancers: Biology and Clinical Management. 2nd ed: WB Saunders. pp 407-419.
2. Jung B, Doctolero RT, Tajima A, Nguyen AK, Keku T, et al. (2004) Loss of activin receptor type 2 protein expression in microsatellite unstable colon cancers. Gastroenterology 126: 654-659.
3. Chung H, Young DJ, Lopez CG, Le TA, Lee JK, et al. (2008) Mutation rates of TGFBR2 and ACVR2 coding microsatellites in human cells with defective DNA mismatch repair. PLoS ONE 3: e3463.
4. Grady WM, Myeroff LL, Swinler SE, Rajput A, Thiagalingam S, et al. (1999) Mutational inactivation of transforming growth factor beta receptor type II in microsatellite stable colon cancers. Cancer Res 59: 320-324.
5. Eppert K, Scherer SW, Ozcelik H, Pirone R, Hoodless P, et al. (1996) MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell 86: 543-552.
6. Schutte M, Hruban RH, Hedrick L, Cho KR, Nadasdy GM, et al. (1996) DPC4 gene in various tumor types. Cancer Res 56: 2527-2530.
7. Takagi Y, Kohmura H, Futamura M, Kida H, Tanemura H, et al. (1996) Somatic alterations of the DPC4 gene in human colorectal cancers in vivo. Gastroenterology 111: 1369-1372.
8. Chen YG, Lui HM, Lin SL, Lee JM, Ying SY (2002) Regulation of cell proliferation, apoptosis, and carcinogenesis by activin. Exp Biol Med (Maywood) 227: 75-87.
9. Lee YJ, Hong KH, Yun J, Oh SP (2006) Generation of activin receptor type IIB isoform-specific hypomorphic alleles. Genesis 44: 487-494.
10. Mori Y, Yin J, Rashid A, Leggett BA, Young J, et al. (2001) Instabilotyping: comprehensive identification of frameshift mutations caused by coding region microsatellite instability. Cancer Res 61: 6046-6049.
11. Hempen PM, Zhang L, Bansal RK, Iacobuzio-Donahue CA, Murphy KM, et al. (2003) Evidence of selection for clones having genetic inactivation of the activin A type II receptor (ACVR2) gene in gastrointestinal cancers. Cancer Res 63: 994-999.
12. Jung BH, Beck SE, Cabral J, Chau E, Cabrera BL, et al. (2007) Activin type 2 receptor restoration in MSI-H colon cancer suppresses growth and enhances migration with activin. Gastroenterology 132: 633-644.
13. Deacu E, Mori Y, Sato F, Yin J, Olaru A, et al. (2004) Activin type II receptor restoration in ACVR2-deficient colon cancer cells induces transforming growth factor-beta response pathway genes. Cancer Res 64: 7690-7696.
14. Jung B, Smith EJ, Doctolero RT, Gervaz P, Alonso JC, et al. (2006) Influence of target gene mutations on survival, stage and histology in sporadic microsatellite unstable colon cancers. Int J Cancer 118: 2509-2513.
15. Satia-Abouta J, Galanko JA, Martin CF, Potter JD, Ammerman A, et al. (2003) Associations of micronutrients with Colon Cancer risk in African Americans and whites: results from the North Carolina Colon Cancer Study. Cancer Epidemiol Biomarkers Prev 12: 747-754.
16. Satia JA, Keku T, Galanko JA, Martin C, Doctolero RT, et al. (2005) Diet, lifestyle, and genomic instability in the North Carolina Colon Cancer Study. Cancer Epidemiol Biomarkers Prev 14: 429-436.
17. Grady WM, Rajput A, Myeroff L, Liu DF, Kwon K, et al. (1998) Mutation of the type II transforming growth factor-beta receptor is coincident with the transformation of human colon adenomas to malignant carcinomas. Cancer Res 58: 3101-3104.
18. Munoz NM, Upton M, Rojas A, Washington MK, Lin L, et al. (2006) Transforming growth factor beta receptor type II inactivation induces the malignant transformation of intestinal neoplasms initiated by Apc mutation. Cancer Res 66: 9837-9844.
19. Olaru A, Mori Y, Yin J, Wang S, Kimos MC, et al. (2003) Loss of heterozygosity and mutational analyses of the ACTRII gene locus in human colorectal tumors. Lab Invest 83: 1867-1871.
20. Deng G, Chen A, Hong J, Chae HS, Kim YS (1999) Methylation of CpG in a small region of the hMLH1 promoter invariably correlates with the absence of gene expression. Cancer Res 59: 2029-2033.
21. Gong J, Ammanamanchi S, Ko TC, Brattain MG (2003) Transforming growth factor beta 1 increases the stability of p21/WAF1/CIP1 protein and inhibits CDK2 kinase activity in human colon carcinoma FET cells. Cancer Res 63: 3340-3346.
22. Carethers JM, Hawn MT, Greenson JK, Hitchcock CL, Boland CR (1998) Prognostic significance of allelic lost at chromosome 18q21 for stage II colorectal cancer. Gastroenterology 114: 1188-1195.
23. Shin SK, Nagasaka T, Jung BH, Matsubara N, Kim WH, et al. (2007) Epigenetic and genetic alterations in Netrin-1 receptors UNC5C and DCC in human colon cancer. Gastroenterology 133: 1849-1857.
24. Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB (1996) Methylation- specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A 93: 9821-9826.
25. Anks SK, Quinn AM, Hunter T (1988) The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241: 42-52.