Simulated microgravity perturbs actin polymerization to promote nitric oxide-associated migration in human immortalized Eahy926 cells

Protoplasma

Volumn 242, Issue 1, 2010, Pages 3-12

  Siamwala, Jamila H ^a   Reddy, S Himabindu ^a   Majumder, Syamantak ^a   Kolluru, Gopi Krishna ^a   Muley, Ajit ^a   Sinha, Swaraj ^a   Chatterjee, Suvro ^a  

^a ANNA UNIVERSITY   (India)

Author keywords

Actin; Endothelial cells; Migration; Nitric oxide; Simulated microgravity

Indexed keywords

ACTIN; ANDROSTANE DERIVATIVE; BRADYKININ; ENDOTHELIAL NITRIC OXIDE SYNTHASE; ENZYME INHIBITOR; MITOGEN ACTIVATED PROTEIN KINASE; NITRIC OXIDE; PHOSPHATIDYLINOSITOL 3 KINASE; WORTMANNIN;

ANGIOGENESIS; ARTICLE; BIOLOGICAL MODEL; BIOSYNTHESIS; CELL LINE; CELL MOTION; CELL SURFACE; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; ENDOTHELIUM CELL; ENZYMOLOGY; HUMAN; INSTRUMENTATION; METABOLISM; WEIGHTLESSNESS; WOUND HEALING;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ACTINS; ANDROSTADIENES; BRADYKININ; CELL LINE; CELL LINE, TRANSFORMED; CELL MOVEMENT; CELL SURFACE EXTENSIONS; ENDOTHELIAL CELLS; ENZYME INHIBITORS; HUMANS; MITOGEN-ACTIVATED PROTEIN KINASES; MODELS, BIOLOGICAL; NEOVASCULARIZATION, PHYSIOLOGIC; NITRIC OXIDE; NITRIC OXIDE SYNTHASE TYPE III; WEIGHTLESSNESS SIMULATION; WOUND HEALING;

EID: 77954027318     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00709-010-0114-z     Document Type: Article

References (57)

1. Bruce A, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002) Molecular biology of the cell, 4th edn. Garland Science, Taylor & Francis Group, New York, p 908, 931, 973-975.
2. Buchen B, Hoson T, Kamisaka S, Masuda Y, Sievers A (1993) Development of statocyte polarity under simulated microgravity on a 3-D clinostat. Biol Sci Space 7: 111-115.
3. Buravkova L, Romanov Y, Rykova M, Grigorieva O, Merzlikina N (2005) Cell-to-cell interactions in changed gravity: ground-based and flight experiments. Acta Astronaut 57: 67-74.
4. Carlsson SI, Bertilaccio MT, Ascari I, Bradamante S, Maier JA (2002) Modulation of human endothelial cell behaviour in simulated microgravity. J Gravit Physiol 9: 273-274.
5. Carlsson SI, Bertilaccio MT, Ballabio E, Maier JA (2003) Endothelial stress by gravitational unloading: effects on cell growth and cytoskeletal organization. Biochim Biophys Acta 1642: 173-179.
6. Carter SB (1967) Effects of cytochalasins on mammalian cells. Nature 213: 261-264.
7. Cines DB, Pollak ES, Buck CA, Loscalzo J, Zimmerman GA, McEver RP, Pober JS, Wick TM, Konkle BA, Schwartz BS, Barnathan ES, McCrae KR, Hug BA, Schmidt AM, Stern DM (1998) Endothelial cells in physiology and in the pathophysiology of vascular disorders. Blood 91: 3527-356.
8. Dimmeler S, Fleming I, Fisslthaler B, Hermann C, Busse R, Zeiher AM (1999) Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. Nature 399: 601-605.
9. Edgell CJ, McDonald CC, Graham JB (1983) Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc Natl Acad Sci USA 80: 3734-3737.
10. Gail MH, Boone CW (1971) Cytochalasin effects on BALB-3T3 fibroblasts: dose-dependent, reversible alteration of motility and cytoplasmic cleavage. Exp Cell Res 68: 226-228.
11. Geary GG, Krause DN, Purdy RE, Duckles SP (1998) Simulated microgravity increases myogenic tone in rat cerebral arteries. J Appl Physiol 85: 1615-1621.
12. Girn HR, Ahilathirunayagam S, Mavor AI, Homer-Vanniasinkam S (2007) Reperfusion syndrome: cellular mechanisms of microvascular dysfunction and potential therapeutic strategies. Vasc Endovascular Surg 41: 277-293.
13. Grimm D, Bauer J, Kossmehl P, Shakibaei M, Schoberger J, Pickenhahn H, Schulze-Tanzil G, Vetter R, Eilles C, Paul M, Cogoli A (2002) Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells. FASEB J 16: 604-606.
14. Gruener R, Roberts R, Reitstetter R (1994) Reduced receptor aggregation and altered cytoskeleton in cultured myocytes after space-flight. Biol Sci Space 8: 79-93.
15. Hattori Y, Kasai K (2004) Disruption of the actin cytoskeleton up-regulates iNOS expression in vascular smooth muscle cells. J Cardio Pharma 43: 209-213.
16. Hirasaka K, Nikawa T, Yuge L, Ishihara I, Higashibata A, Ishioka N, Okubo A, Miyashita T, Suzue N, Ogawa T, Oarada M, Kishi K (2005) Clinorotation prevents differentiation of rat myoblastic L6 cells in association with reduced NF-kappa B signaling. Biochim Biophys Acta 1743: 130-140.
17. Huang C, Jacobson K, Schaller MD (2004) MAP kinases and cell migration. J Cell Sci 117: 4619-4628.
18. Hughes-Fulford M, Nelson K, Blaug S, Summer CG, Lukefahr BD, Lewis ML (1993) MC3T3 osteoblasts grown in microgravity on STS-56 have reduced cell growth, glucose utilization with altered actin cytoskeleton and increased prostaglandin synthesis. ASGSB Bulletin 7: 31.
19. Huijser RH (2000) Desktop RPM: new small size microgravity simulator for the bioscience laboratory. FS-MG-0017, Fokker Space.
20. Ichigi J, Asashima M (2002) Dome formation and tubule morphogenesis by Xenopus kidney A6 cell cultures exposed to microgravity simulated with a 3D-clinostat and to hypergravity. In Vitro Cell Dev Biol, Anim 37: 31-44.
21. Ingber DE (1999) How cells (might) sense gravity. FASEB J 13: S3-S15.
22. Ji Y, Ferracci G, Warley A, Ward M, Leung KY, Samsuddin S, Lévêque C, Queen L, Reebye V, Pal P, Gkaliagkousi E, Seager M, Ferro A (2007) β-Actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90. Proc Natl Acad Sci USA 104: 8839-8844.
23. Kawasaki K, Smith RS Jr, Hsieh CM, Sun J, Chao J, Liao JK (2003) Activation of the phosphatidylinositol 3-kinase/protein kinase Akt pathway mediates nitric oxide-induced endothelial cell migration and angiogenesis. Mol Cell Biol 23: 5726-5737.
24. Kumei Y, Morita S, Katano H, Akiyama H, Hirano M, Oyha K, Shimokawa H (2006) Microgravity signal ensnarls cell adhesion, cytoskeleton, and matrix proteins of rat osteoblasts: osteopontin, CD44, osteonectin, and alpha-tubulin. Ann N Y Acad Sci 1090: 311-317.
25. Lee JS, Kang-Decker N, Chatterjee S, Yao J, Friedman S, Shah V (2005) Mechanisms of nitric oxide interplay with Rho GTPase family members in modulation of actin membrane dynamics in pericytes and fibroblasts. Am J Pathol 166: 1861-1870.
26. Lewis ML, Reynolds JL, Cubano LA, Hatton JP, Lawless BD, Piepmeier EH (1998) Spaceflight alters microtubules and increases apoptosis in human lymphocytes (Jurkat). FASEB J 12: 1007-1018.
27. Li Z, Song Y, Ma Y, Wei H, Liu C, Huang J, Wang N, Sha J, Sakurai F (2002) Influence of simulated microgravity on avian primordial germ cell migration and reproductive capacity. J Exp Zool 292: 672-676.
28. Li S, Huang NF, Hsu S (2005) Mechanotransduction in endothelial cell migration. J Cell Biochem 96: 1110-1126.
29. Ma J, Kahwaji CI, Ni Z, Vaziri ND, Purdy RE (2003) Effects of simulated microgravity on arterial nitric oxide synthase and nitrate and nitrite content. J Appl Physiol 94: 83-92.
30. Martin SJ, Cotter TG (1990) Disruption of microtubules induces an endogenous suicide pathway in human leukaemia HL-60 cells. Cell Tissue Kinet 23: 545-559.
31. Nims RW, Cook JC, Krishna MC, Christodoulou D, Poore CM, Miles AM (1996) Colorimetric assays for nitric oxide and nitrogen oxide species formed from nitric oxide stock solutions and donor compounds. Methods Enzymol 268: 93-105.
32. Papaseit C, Pochon N, Tabony J (2000) Microtubule self-organization is gravity-dependent. Proc Natl Acad Sci USA 8: 8364-8368.
33. Pfeffer W (1990) Pflanzenphysiologie. 2. Aufl. Engelmann Leipzig.
34. Plett PA, Abonour R, Frankovitz SM, Orschell CM (2004) Impact of modeled microgravity on migration, differentiation and cell cycle control of primitive human hematopoietic progenitor cells. Exp Hematol 32: 773-781.
35. Romanov IuA, Kabaeva NV, Buravkova LB (2001) Alterations in actin cytoskeleton and rate of reparation of human endothelium (the wound-healing model) under the condition of clinostatting. Aviakosm Ekolog Med 35: 37-40.
36. Ross R (1999) Atherosclerosis-an inflammatory disease. N Engl J Med 340: 115-126.
37. Rousseau S, Houle F, Landry J, Huot J (1997) p38 MAP kinase activation by vascular endothelial growth factor mediates actin reorganization and cell migration in human endothelial cells. Oncogene 15: 2169-2177.
38. Rubanyi GM (1993) The role of endothelium in cardiovascular homeostasis and diseases. J Cardiovasc Pharmacol 22: S1-S4.
39. Sachs J (1882) Ueber Ausschliessung der geotropischen und heliotropischen Krummungen wahrend des Wachsens. Arb Bot Inst Wurzburg Bd 2: 209-225.
40. Sakar D, Nagaya T, Koga K, Seo H (1999) Culture in vector-averaged gravity environment in a clinostat results in detachment of osteoblastic ROS 17/2. 8. Cells Environmental Medicine 43: 22-24.
41. Sampath P, Pollard TD (1991) Effects of cytochalasin, phalloidin and pH on the elongation of actin filaments. Biochemistry 30: 1973-1980.
42. Sarkar D, Nagaya T, Koga K, Nomura Y, Gruener R, Seo H (2000) Culture in vector-averaged gravity under clinostat rotation results in apoptosis of osteoblastic ROS 17/2. 8 cells. J Bone Miner Res 15: 489-498.
43. Searles CD, Ide L, Davis ME, Cai H, Weber M (2004) Actin cytoskeleton organization and posttranscriptional regulation of endothelial nitric oxide synthase during cell growth. Circ Res 95: 488-495.
44. Sessa WC (2005) Regulation of endothelial derived nitric oxide in health and disease. Mem Inst Oswaldo Cruz 1: 15-18.
45. Small VJ, Vignala E, Rottnerb K (2002) The lamellipodium: where motility begins. Trends Cell Biol 12: 112-120.
46. Spooner BS, Yamada KM, Wessells NK (1971) Microfilaments and cell locomotion. J Cell Biol 49: 595-613.
47. Su Y, Edwards-Bennett S, Bubb MR, Block ER (2003) Block. Regulation of endothelial nitric oxide synthase by the actin cytoskeleton. Am J Physiol Cell Physiol 284: 1542-1549.
48. Sundaresan A, Risin D, Pellis NR (2002) Loss of signal transduction and inhibition of lymphocyte locomotion in a ground-based model of microgravity. In Vitro Cell Dev Biol, Anim 38: 118-122.
49. Takahashi K, Sawasaki Y, Hata J, Mukai K, Goto T (1990) Spontaneous transformation and immortalization of human endothelial cells. In Vitro Cell Dev Biol 26: 265-74.
50. Uva BM, Masini MA, Sturla M, Prato P, Passalacqua M, Giuliani M, Tagliafierro G, Strollo F (2002) Clinorotation-induced weightlessness influences the cytoskeleton of glial cells in culture. Brain Res 934: 132-139.
51. van Hinsbergh VWM (1996) Vascular control of hemostasis, advances in vascular biology, vol 1. Harwood Academic Publishers, New York.
52. Versari S, Villa A, Bradamante S, Maier JA (2007) Alterations of the actin cytoskeleton and increased nitric oxide synthesis are common features in human primary endothelial cell response to changes in gravity. Biochim Biophys Acta 1773: 1645-52.
53. Walther I, Pippia P, Meloni MA, Turrini F, Mannu F, Cogoli A (1998) Simulated microgravity inhibits the genetic expression of interleukin-2 and its receptor in mitogen-activated T lymphocytes. FEBS Lett 436: 115-118.
54. Wessells NK, Spooner BS, Ash JF, Bradley MO, Luduena MA, Taylor EL, Wrenn JT, Yamaa K (1971) Microfilaments in cellular and developmental processes. Science 171: 135-43.
55. Woods CC, Banks KE, Gruener R, DeLuca D (2003) Loss of T cell precursors after spaceflight and exposure to vector-averaged gravity. FASEB J 17: 1526-1528.
56. Yates BJ, Kerman IA (1998) Post-spaceflight orthostatic intolerance: possible relationship to microgravity-induced plasticity in the vestibular system. Brain Res Brain Res Rev 28: 73-82.
57. Zeng C, Morrison AR (2001) Disruption of the actin cytoskeleton regulates cytokine-induced iNOS expression. Am J Physiol Cell Physiol 281: C932-C940.