Extracellular secretion in Bacillus subtilis of a cytoplasmic thermostable β-galactosidase from Geobacillus stearothermophilus

Journal of Dairy Science

Volumn 93, Issue 7, 2010, Pages 2838-2845

  Xia, Y ^a   Zhao, J ^a   Chen, H ^a   Liu, X ^a   Wang, Y ^a   Tian, F ^a   Zhang, H P ^b   Zhang, H ^a   Chen, W ^a  

^a JIANGNAN UNIVERSITY   (China)

^b INNER MONGOLIA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Bacillus subtilis; Secretion; Thermostable galactosidase

Indexed keywords

BETA GALACTOSIDASE; PHOSPHODIESTERASE; SIGNAL PEPTIDE;

ARTICLE; BACILLUS SUBTILIS; DAIRYING; ENZYMOLOGY; GENETICS; GEOBACILLUS STEAROTHERMOPHILUS; METABOLISM; METHODOLOGY; PHYSIOLOGY; SECRETION;

BACILLUS SUBTILIS; BETA-GALACTOSIDASE; DAIRYING; GEOBACILLUS STEAROTHERMOPHILUS; PHOSPHORIC DIESTER HYDROLASES; PROTEIN SORTING SIGNALS;

BACILLUS SUBTILIS; GEOBACILLUS STEAROTHERMOPHILUS; LURIA;

EID: 77953910331     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2009-2864     Document Type: Article

References (30)

1. Barnett J.P., van der Ploeg R., Eijlander R.T., Nenninger A., Mendel S., Rozeboom R., Kuipers O.P., van Dijl J.M., Robinson C. The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements. FEBS J. 2009, 276:232-243.
2. Barrett C.M., Ray N., Thomas J.D., Robinson C., Bolhuis A. Quantitative export of a reporter protein, GFP, by the twin-arginine translocation pathway in Escherichia coli. Biochem. Biophys. Res. Commun. 2003, 304:279-284.
3. Berks B.C., Sargent F., Palmer T. The Tat protein export pathway. Mol. Microbiol. 2000, 35:260-274.
4. Brüser T. The twin-arginine translocation system and its capability for protein secretion in biotechnological protein production. Appl. Microbiol. Biotechnol. 2007, 76:35-45.
5. Chen W., Chen H., Xia Y., Zhao J., Tian F., Zhang H. Production, purification, and characterization of a potential thermostable galactosidase for milk lactose hydrolysis from Bacillus stearothermophilus. J. Dairy Sci. 2008, 91:1751-1758.
6. DeLisa M.P., Lee P., Palmer T., Georgiou G. Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway. J. Bacteriol. 2004, 186:366-373.
7. Eder S., Shi L., Jensen K., Yamane K., Hulett F.M. A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD. Microbiol. 1996, 142:2041-2047.
8. Gerlach R., Pop O., Müller J.P. Tat dependent export of E. coli phytase AppA by using the PhoD-specific transport system of Bacillus subtilis. J. Basic Microbiol. 2004, 44:351-359.
9. Gote M., Umalkar H., Khan I., Khire J. Thermostable α-galactosidase from Bacillus stearothermophilus (NCIM 5146) and its application in the removal of flatulence causing factors from soymilk. Process Biochem. 2004, 39:1723-1729.
10. Harwood C.R., Cutting S.M. Molecular Biological Methods for Bacillus 1990, Wiley, Sussex, UK.
11. Hirata H., Negoro S., Okada H. Molecular basis of isozyme formation of β-galactosidases in Bacillus stearothermophilus: Isolation of two β-galactosidase genes, bgaA and bgaB. J. Bacteriol. 1984, 160:9-14.
12. Hirata H., Negoro S., Okada H. High production of thermostable β-galactosidase of Bacillus stearothermophilus in Bacillus subtilis. Appl. Environ. Microbiol. 1985, 49:1547-1549.
13. Jongbloed J.D.H., Grieger U., Antelmann H., Hecker M., Nijland R., Bron S., van Dijl J.M. Two minimal Tat translocases in Bacillus. Mol. Microbiol. 2004, 54:1319-1325.
14. Jongbloed J.D.H., Martin U., Antelmann H., Hecker M., Tjalsma H., Venema G., Bron S., van Dijl J.M., Müller J. TatC is a specificity determinant for protein secretion via the twin-arginine translocation pathway. J. Biol. Chem. 2000, 275:41350-41357.
15. Kang J.H., Kim S.J., Park Y.C., Hwang Y., Yoo O.J., Kim Y.C. Nucleotide sequence of the penicillin G acylase gene from Bacillus megaterium and characteristics of the enzyme. Misainmurhag. Hoiji. 1994, 32:215-221.
16. Kikuchi Y., Itaya H., Date M., Matsui K., Wu L.F. TatABC overexpression improves Corynebacterium glutamicum Tat-dependent protein secretion. Appl. Environ. Microbiol. 2009, 75:603-607.
17. Kolkman M.A.B., van der Ploeg R., Bertels M., van Dijk M., van der Laan J., van Dijl J.M., Ferrari E. The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: Secretion of active subtilisin via the B. subtilis Tat pathway. Appl. Environ. Microbiol. 2008, 74:7507-7513.
18. Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessières P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.K., Codani J.J., Connerton I.F., Danchin A., et al. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 1997, 390:249-256.
19. Li W., Zhou X., Lu P. Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis. Res. Microbiol. 2004, 155:605-610.
20. Müller J.P., An Z.D., Merad T., Hancock I.C., Harwood R.C. Influence of Bacillus subtilis phoR on cell wall anionic polymers. Microbiol. 1997, 43:947-956.
21. d proteins of Bacillus subtilis form an autonomous Tat translocation system. J. Biol. Chem. 2002, 277:3268-3273.
22. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York, NY. 3rd ed.
23. Smith T.J., Blackman S.A., Foster S.J. Autolysins of Bacillus subtilis: Multiple enzymes with multiple functions. Microbiol. 2000, 146:249-262.
24. Tjalsma H., Antelmann H., Jongbloed J.D.H., Braun P.G., Darmon E., Dorenbos R., Dubois J.-Y.F., Westers H., Zanen G., Quax W.J., Kuipers O.P., Bron S., Hecker M., van Dijl J.M. Proteomics of protein secretion by Bacillus subtilis: Separating the " secrets" of the secretome. Microbiol. Mol. Biol. Rev. 2004, 68:207-233.
25. Tjalsma H., Bolhuis A., Jongbloed J.D.H., Bron S., van Dijl J.M. Signal peptide-dependent protein transport in Bacillus subtilis: A genome-based survey of the secretome. Microbiol. Mol. Biol. Rev. 2000, 9:515-547.
26. van Dijl J.M., Braun P.G., Robinson C., Quax W.J., Antelmann H., Hecker M., Müller J., Tjalsma H., Bron S., Jongbloed J.D.H. Functional genomic analysis of the Bacillus subtilis Tat pathway for protein secretion. J. Biotechnol. 2002, 98:243-254.
27. Wanarska M., Kur J., Pladzyk R., Turkiewicz M. Thermostable Pyrococcus woesei β-d-galactosidase-high level expression, purification and biochemical properties. Acta Biochim. Pol. 2005, 52:781-787.
28. Westers L., Dijkstra D.S., Westers H., van Dijl J.M., Quax W.J. Secretion of functional human interleukin-3 from Bacillus subtilis. J. Biotechnol. 2006, 123:211-224.
29. Xia Y., Chen W., Fu X.Y., Zhang H., Yang S., Ding X.L. Construction of an integrative food-grade expression system for Bacillus subtilis. Food Res. Int. 2005, 38:251-256.
30. A-like promoter and the roles of the inverted repeat sequence (CIRCE). J. Bacteriol. 1995, 177:5427-5433.